LOCUS       pLVX-FUSN15YS-mC       14234 bp DNA     circular SYN 04-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 10, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..14234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3063..3083
                     /label=SV40 NLS
     misc_feature    3093..3320
                     /label=cBioTap
     misc_feature    3336..3924
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             3941..4459
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.3"
                     /note="/match_length=84.0"
                     /note="/other=CDS"
                     /translation="SRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRP
                     MTYKAAVDLSHFLKEKRGLEGLIHSQRRQDILDLWIYHTQGYFPD*QNYTPGPGVRYPL
                     TFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVAR
                     ELHPEYFKNC"
     LTR             4507..4764
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     protein_bind    complement(4914..4936)
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     promoter        complement(4940..4970)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(4985..5006)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(5019..5111)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(5294..5882)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6053..6913)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6914..7018)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     polyA_signal    7066..7200
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     LTR             7368..7402
                     /label=3' LTR (Delta-U3) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=15.0"
                     /note="/other=LTR"
     LTR             7744..8001
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    8048..8173
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             8157..8495
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVR"
     misc_feature    8670..8903
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             9280..9380
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    9395..9510
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     enhancer        9550..9853
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        9854..10057
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             10091..10690
                     /codon_start=1
                     /label=FUSNmut
                     /label=FUS (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=75.0"
                     /note="/match_length=38.0"
                     /note="/other=CDS"
                     /translation="MASNDYTQQATQSSGASPTQPGQGYSQQSSQPSGQQSSSGSSQST
                     DTSGYGQSSSSSSGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSSGQQSSSPGSGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSSSQQPSYGGQQQSSGQQQSSNPPQGSGQQNQSNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQS"
     CDS             10754..11461
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             11486..12997
                     /codon_start=1
                     /label=CRY2Clust
                     /label=CRY2 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.2"
                     /note="/match_length=82.4"
                     /note="/other=CDS"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAARDPPD"
     CDS             13091..13135
                     /codon_start=1
                     /label=AviTag(TM)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="GLNDIFEAQKIEWHE"
     CDS             join(13187..14234,1..3053)
                     /codon_start=1
                     /label=dCas9
                     /label=cas9
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
BASE COUNT         3822 a         3494 c         3737 g         3181 t
ORIGIN
        1 tctttgacca gtcaaagaat ggatacgccg gctacatcga cggaggtgcc tcccaagagg
       61 aattttataa gtttatcaaa cctatccttg agaagatgga cggcaccgaa gagctcctcg
      121 tgaaactgaa tcgggaggat ctgctgcgga agcagcgcac tttcgacaat gggagcattc
      181 cccaccagat ccatcttggg gagcttcacg ccatccttcg gcgccaagag gacttctacc
      241 cctttcttaa ggacaacagg gagaagattg agaaaattct cactttccgc atcccctact
      301 acgtgggacc cctcgccaga ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag
      361 aaactatcac tccctggaac ttcgaagagg tggtggacaa gggagccagc gctcagtcat
      421 tcatcgaacg gatgactaac ttcgataaga acctccccaa tgagaaggtc ctgccgaaac
      481 attccctgct ctacgagtac tttaccgtgt acaacgagct gaccaaggtg aaatatgtca
      541 ccgaagggat gaggaagccc gcattcctgt caggcgaaca aaagaaggca attgtggacc
      601 ttctgttcaa gaccaataga aaggtgaccg tgaagcagct gaaggaggac tatttcaaga
      661 aaattgaatg cttcgactct gtggagatta gcggggtcga agatcggttc aacgcaagcc
      721 tgggtaccta ccatgatctg cttaagatca tcaaggacaa ggattttctg gacaatgagg
      781 agaacgagga catccttgag gacattgtcc tgactctcac tctgttcgag gaccgggaaa
      841 tgatcgagga gaggcttaag acctacgccc atctgttcga cgataaagtg atgaagcaac
      901 ttaaacggag aagatatacc ggatggggac gccttagccg caaactcatc aacggaatcc
      961 gggacaaaca gagcggaaag accattcttg atttccttaa gagcgacgga ttcgctaatc
     1021 gcaacttcat gcaacttatc catgatgatt ccctgacctt taaggaggac atccagaagg
     1081 cccaagtgtc tggacaaggt gactcactgc acgagcatat cgcaaatctg gctggttcac
     1141 ccgctattaa gaagggtatt ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga
     1201 tgggtcgcca taaaccagag aacattgtca tcgagatggc cagggaaaac cagactaccc
     1261 agaagggaca gaagaacagc agggagcgga tgaaaagaat tgaggaaggg attaaggagc
     1321 tcgggtcaca gatccttaaa gagcacccgg tggaaaacac ccagcttcag aatgagaagc
     1381 tctatctgta ctaccttcaa aatggacgcg atatgtatgt ggaccaagag cttgatatca
     1441 acaggctctc agactacgac gtggacgcca tcgtccctca gagcttcctc aaagacgact
     1501 caattgacaa taaggtgctg actcgctcag acaagaaccg gggaaagtca gataacgtgc
     1561 cctcagagga agtcgtgaaa aagatgaaga actattggcg ccagcttctg aacgcaaagc
     1621 tgatcactca gcggaagttc gacaatctca ctaaggctga gaggggcgga ctgagcgaac
     1681 tggacaaagc aggattcatt aaacggcaac ttgtggagac tcggcagatt actaaacatg
     1741 tcgcccaaat ccttgactca cgcatgaata ccaagtacga cgaaaacgac aaacttatcc
     1801 gcgaggtgaa ggtgattacc ctgaagtcca agctggtcag cgatttcaga aaggactttc
     1861 aattctacaa agtgcgggag atcaataact atcatcatgc tcatgacgca tatctgaatg
     1921 ccgtggtggg aaccgccctg atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg
     1981 gagactacaa ggtctacgac gtgcgcaaga tgattgccaa atctgagcag gagatcggaa
     2041 aggccaccgc aaagtacttc ttctacagca acatcatgaa tttcttcaag accgaaatca
     2101 cccttgcaaa cggtgagatc cggaagaggc cgctcatcga gactaatggg gagactggcg
     2161 aaatcgtgtg ggacaagggc agagatttcg ctaccgtgcg caaagtgctt tctatgcctc
     2221 aagtgaacat cgtgaagaaa accgaggtgc aaaccggagg cttttctaag gaatcaatcc
     2281 tccccaagcg caactccgac aagctcattg caaggaagaa ggattgggac cctaagaagt
     2341 acggcggatt cgattcacca actgtggctt attctgtcct ggtcgtggct aaggtggaaa
     2401 aaggaaagtc taagaagctc aagagcgtga aggaactgct gggtatcacc attatggagc
     2461 gcagctcctt cgagaagaac ccaattgact ttctcgaagc caaaggttac aaggaagtca
     2521 agaaggacct tatcatcaag ctcccaaagt atagcctgtt cgaactggag aatgggcgga
     2581 agcggatgct cgcctccgct ggcgaacttc agaagggtaa tgagctggct ctcccctcca
     2641 agtacgtgaa tttcctctac cttgcaagcc attacgagaa gctgaagggg agccccgagg
     2701 acaacgagca aaagcaactg tttgtggagc agcataagca ttatctggac gagatcattg
     2761 agcagatttc cgagttttct aaacgcgtca ttctcgctga tgccaacctc gataaagtcc
     2821 ttagcgcata caataagcac agagacaaac caattcggga gcaggctgag aatatcatcc
     2881 acctgttcac cctcaccaat cttggtgccc ctgccgcatt caagtacttc gacaccacca
     2941 tcgaccggaa acgctatacc tccaccaaag aagtgctgga cgccaccctc atccaccaga
     3001 gcatcaccgg actttacgaa actcggattg acctctcaca gctcggaggg gatgagggag
     3061 ctcccaagaa aaagcgcaag gtaggtagtt ccgccggaaa ggccggtgaa ggtgaaatcc
     3121 ctgcccctct tgctggtacc gtttctaaga tactggtaaa agaaggtgac actgttaaag
     3181 ctggtcaaac agttctggtg ctggaggcta tgaaaatgga gacagaaatt aacgctccta
     3241 ctgacggaaa agttgaaaag gtgttagtta aggaaagaga tgctgttcaa ggtggtcaag
     3301 gtctaatcaa gatcggcgtt tagcgcgtct ggaacaatca acctctggat tacaaaattt
     3361 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3421 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3481 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3541 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3601 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3661 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3721 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     3781 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3841 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3901 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     3961 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     4021 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     4081 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     4141 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     4201 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     4261 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     4321 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     4381 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     4441 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     4501 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     4561 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4741 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     4801 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt
     4861 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4921 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4981 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5041 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5101 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5161 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5221 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5281 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5341 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5401 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5461 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5521 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5581 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5641 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5701 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5761 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5821 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5881 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5941 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6001 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6061 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6361 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6481 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6661 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6901 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7021 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
     7081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     7201 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     7261 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     7321 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     7381 tcactcccaa agaagacaag atatccttga tttgtggatc taccacacac aaggctactt
     7441 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     7501 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     7561 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     7621 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     7681 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     7741 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     7801 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7861 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7921 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7981 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8041 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8101 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8161 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8221 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8281 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8341 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8401 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8461 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     8521 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8581 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8641 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8701 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8761 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8821 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8881 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8941 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9001 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9061 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9121 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9181 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9241 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9301 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     9361 attcgattag tgaacggatc tcgacggtat cgcctttaaa agaaaagggg ggattggggg
     9421 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9481 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     9541 agtttatcgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     9601 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     9661 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     9721 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     9781 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     9841 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     9901 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     9961 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
    10021 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
    10081 ctaggggccc atggcctcaa acgattatac ccaacaagca acccaaagct ctggggcctc
    10141 tcccacccag cccgggcagg gctattccca gcagagcagt cagccctctg gacagcagag
    10201 ttctagtggt tctagccagt ccacggacac ttcaggctat ggccagagca gctcttcttc
    10261 ttctggccag agccagaaca caggctatgg aactcagtca actccccagg gatatggctc
    10321 gactggcggc tatggcagta gccagagctc ccaatcgtct tctgggcagc agtcctcctc
    10381 tcctggctct ggccagcagc cagctcccag cagcacctcg ggaagttacg gtagcagttc
    10441 tcagagcagc agctatgggc agccccagag tgggagctct agccagcagc ctagctatgg
    10501 tggacagcag caaagctctg gacagcagca aagctctaat ccccctcagg gctctggaca
    10561 gcagaaccag tctaacagca gcagtggtgg tggaggtgga ggtggaggtg gaggtaacta
    10621 tggccaagat caatcctcca tgagtagtgg tggtggcagt ggtggcggtt atggcaatca
    10681 agaccagagt ggtggaggtg gcagcggtgg ctatggacag caggaccgtg gaggtggagg
    10741 aactagtgct agcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
    10801 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
    10861 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
    10921 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
    10981 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
    11041 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
    11101 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
    11161 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
    11221 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
    11281 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
    11341 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
    11401 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
    11461 gggtagcgga ccaccggtcg ccaccatgaa gatggacaaa aagaccatcg tctggtttcg
    11521 gagagatttg agaatagaag ataatcccgc gctcgccgcc gcggcccacg agggttccgt
    11581 cttccccgtt ttcatttggt gtcctgaaga agaaggccag ttttatcccg gaagggcctc
    11641 taggtggtgg atgaagcaaa gtctggccca tcttagccag tcactgaaag cactgggcag
    11701 tgatcttacc ctgatcaaga cacacaatac catctctgcc atcctcgact gcatcagggt
    11761 gaccggcgca acgaaagtcg tgtttaacca cctgtacgat ccagttagtc tggtgcgcga
    11821 ccacactgtg aaggagaagc tggtggaacg ggggatcagt gtgcagagct acaacgggga
    11881 ccttctgtac gagccatggg agatctattg cgagaaaggg aaaccgttca cctccttcaa
    11941 cagttactgg aagaaatgtt tggatatgtc aatagagtcc gttatgttgc cccctccctg
    12001 gagactgatg ccgattactg ctgctgcaga ggccatctgg gcctgctcca tcgaggaact
    12061 cggtctggaa aatgaagcag aaaagccaag caatgcactt ctcactagag cctggagccc
    12121 cggctggtct aatgccgaca agctgcttaa cgagttcatc gaaaaacaac tgattgacta
    12181 cgcgaagaac tccaagaaag tggtaggtaa ctcaactagc ttgctctctc catatctcca
    12241 ttttggcgag atttctgtcc gccatgtatt tcagtgcgct cggatgaaac agattatctg
    12301 ggctcgcgat aaaaacagcg aaggcgaaga aagcgccgat ctgttcctgc gagggatcgg
    12361 acttcgggaa tactcccggt atatatgttt caactttcca ttcacacacg agcagagtct
    12421 gttgtcccac ctcaggttct tcccctggga cgccgatgtc gacaaattca aggcatggag
    12481 acagggaagg acagggtacc cactcgtgga tgctggcatg agagagctct gggctacagg
    12541 ctggatgcac aaccgcatcc gggtaatcgt gtcctcattt gctgtcaagt ttctgctcct
    12601 gccttggaaa tggggaatga agtacttttg ggataccctt ctcgacgccg acttggagtg
    12661 tgacattctg ggatggcaat atattagcgg gtcaattcct gacggccatg agttggacag
    12721 gttggacaat ccggccttgc agggagctaa gtatgatccc gaaggagagt atattcgaca
    12781 gtggctcccc gagctggccc gacttcctac ggagtggatt caccatcctt gggacgcacc
    12841 actgacagtg ctcaaggcaa gcggggtgga gctgggcacc aattacgcta agcctatagt
    12901 tgatatagat acagcacgcg agctgctggc taaagcgatc tctcgcactc gggaggcgca
    12961 gattatgatc ggtgctgccg caagggaccc tcctgatctt gacaatgtgt acagtggggg
    13021 aggcggaggg ggtggttcag gtggtggtgg tggtggtggt tcgggtggtg gcggaggggg
    13081 aggctccggg ggcctgaacg acatcttcga ggctcagaaa atcgaatggc acgaaggcgc
    13141 gccgagctcg aggatccttg ctagccccaa aaagaagagg aaagtggaca agaagtattc
    13201 tatcggactg gccatcggga ctaatagcgt cgggtgggcc gtgatcactg acgagtacaa
    13261 ggtgccctct aagaagttca aggtgctcgg gaacaccgac cggcattcca tcaagaaaaa
    13321 tctgatcgga gctctcctct ttgattcagg ggagaccgct gaagcaaccc gcctcaagcg
    13381 gactgctaga cggcggtaca ccaggaggaa gaaccggatt tgttaccttc aagagatatt
    13441 ctccaacgaa atggcaaagg tcgacgacag cttcttccat aggctggaag aatcattcct
    13501 cgtggaagag gataagaagc atgaacggca tcccatcttc ggtaatatcg tcgacgaggt
    13561 ggcctatcac gagaaatacc caaccatcta ccatcttcgc aaaaagctgg tggactcaac
    13621 cgacaaggca gacctccggc ttatctacct ggccctggcc cacatgatca agttcagagg
    13681 ccacttcctg atcgagggcg acctcaatcc tgacaatagc gatgtggata aactgttcat
    13741 ccagctggtg cagacttaca accagctctt tgaagagaac cccatcaatg caagcggagt
    13801 cgatgccaag gccattctgt cagcccggct gtcaaagagc cgcagacttg agaatcttat
    13861 cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg aacctgattg ctctttcact
    13921 tgggctgact cccaatttca agtctaattt cgacctggca gaggatgcca agctgcaact
    13981 gtccaaggac acctatgatg acgatctcga caacctcctg gcccagatcg gtgaccaata
    14041 cgccgacctt ttccttgctg ctaagaatct ttctgacgcc atcctgctgt ctgacattct
    14101 ccgcgtgaac actgaaatca ccaaggcccc tctttcagct tcaatgatta agcggtatga
    14161 tgagcaccac caggacctga ccctgcttaa ggcactcgtc cggcagcagc ttccggagaa
    14221 gtacaaggaa atct
//
