LOCUS       pTn7-SCOUT22.YB         6451 bp    DNA     circular UNA 11-APR-2023
DEFINITION  F1-J23104::YFP2
            (pOGG303)-R2-J23104::mTag(pOGG302)-ELE2(pOGG273)-pOGG304
            (pL2V-Lv2--pUC18R6KT-miniTn7T-Km) [pOPS1655].
ACCESSION   urn.local.local01.2o-g4v9itc
VERSION     urn.local.local01.2o-g4v9itc
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     oriT            1..109
                     /note="incP origin of transfer"
     rep_origin      160..516
     CDS             complement(925..1785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(1786..1890)
                     /gene="bla"
                     /note="AmpR promoter"
     mobile_element  2321..2519
                     /note="right end of Tn7"
                     /mobile_element_type="transposon:Tn7R"
     regulatory      2568..2662
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0"
                     /note="Geneious type: terminator"
     regulatory      2759..2856
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1"
                     /note="Geneious type: terminator"
     gene            2897..2944
     CDS             complement(2999..3814)
     CDS             complement(2999..3814)
                     /product="neomycin phosphotransferase"
                     /gene="neo"
     gene            3955..4002
     misc_feature    4079..4113
                     /note="Geneious type: promoter prokaryotic"
     RBS             4117..4143
                     /note="Geneious type: rbs"
     gene            4145..4864
     terminator      4872..>4943
                     /note="DT16"
     terminator      complement(<4960..5031)
                     /note="DT16"
     gene            complement(5039..>5737)
     RBS             complement(5742..5768)
                     /note="Geneious type: rbs"
     misc_feature    complement(5772..5806)
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    5815..5834
                     /note="Geneious type: Link"
     terminator      5844..5946
     mobile_element  5983..6148
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN      
        1 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
       61 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccga taagctctga
      121 taacgcagga aagaacatgg gcagttcaac ctgttgatag tacgtactaa gctctcatgt
      181 ttcacgtact aagctctcaa gtttaacgta ctaagctctc atgtttaacg aactaaaccc
      241 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact
      301 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg
      361 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac
      421 ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc
      481 aattttgagt gacacaggaa cacttaacgg ctgacaaccg gctggtaaca ggattagcag
      541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      601 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     1081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     1921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     1981 tcgtctaagc agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     2041 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     2101 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     2161 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     2221 gtgcgggcct cttcgctatt acgccagcta gaggaccagc cgcgtaacct ggcaaaatcg
     2281 gttacggttg agtaataaat ggatgccctg cgtaagcagg tgtgggcgga caataaagtc
     2341 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
     2401 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
     2461 aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggg
     2521 gttcgaggtc gacggtatcg ataagctagc ttaattagct gagcttggac tcctgttgat
     2581 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     2641 ggcgtttttt attggtgaga atccaagcta gactgcgatg agtggcaggg cggggcgtaa
     2701 tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag
     2761 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2821 gtcggtgaac gctctcctga gtaggacaaa tccgccgcta ggagcttgcg gcccggacga
     2881 tcgaattggg gatcttgaag tacctattcc gaagttccta ttctctagaa agtataggaa
     2941 cttcagagcg cttttgaagc tgatgtgctt aaaaacttac tcttagttca attaattatt
     3001 agaaaaattc atccagcatc agatgaaatt gcagtttgtt catatccgga ttatcaatgc
     3061 catatttctg aaacagacgt ttttgcaggc tcgggctaaa ttcgcccagg cagttccaca
     3121 gaatggccag atcctgataa cgatccgcaa tgcccacacg gcccacatca atgcagccaa
     3181 tcagtttgcc ttcatcgaaa atcaggttat ccaggctaaa atcgccgtgg gtcaccacgc
     3241 tatccgggct aaacggcagc agtttatgca tttctttcca cacctgttcc accggccagc
     3301 cgttacgttc atcatcaaaa tcgctcgcat ccaccaggcc gttgttcata cggctctgcg
     3361 cctgggccag acgaaacaca cgatcgctgt taaacgggca gttgcacacc ggaatgctat
     3421 gcagacgacg cagaaacacg gccagcgcat ccacaatgtt ttcgccgcta tccggatatt
     3481 cttccagcac ctgaaacgcg gttttgcccg gaatcgcggt ggtcagcagc cacgcatcat
     3541 ccggggtgcg aataaaatgt ttaatggtcg gcagcggcat aaattcggtc agccagttca
     3601 gacgcaccat ttcatcggtc acatcgttcg ccacgctgcc tttgccatgt ttcagaaaca
     3661 gttccggcgc atccggtttg ccatacagac gataaatggt cgcgccgctc tgacccacgt
     3721 tatcacgcgc ccatttatag ccatacagat ccgcatccat gttgctgttc agacgcggac
     3781 ggctacagct cgtttcacgc tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     3841 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     3901 gattttgaga cacaattgct ccattgctgt tgacaaaggg aatcagggga tcttgaagtt
     3961 cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagcta
     4021 attcgagatc atgcataatg ccgaattcgg atccggagtg ccgaattcgg atccggagtt
     4081 gacagctagc tcagtcctag gtattgtgct agctactcta gaattaaaga ggagaaatta
     4141 accaatggtt agcaagggcg aagaactttt tacaggcgta gtaccgatct tagttgaatt
     4201 agacggcgac gttaacggtc ataagtttag cgtgagcggt gagggtgaag gtgacgcaac
     4261 ttacggcaag ctgaccctga agctgatttg cacgacgggt aagctgccgg tcccgtggcc
     4321 taccctggtc acgaccttgg gttatggcgt tcagtgtttc gcgcgttatc cggaccacat
     4381 gaaacaacac gatttcttta agagcgcgat gccagaaggc tatgtgcagg agcgtacgat
     4441 ctttttcaaa gacgacggta actacaagac gcgtgccgaa gtcaaattcg aaggcgacac
     4501 cctggtgaat cgcattgagc tgaagggtat tgatttcaaa gaggatggca atatcctggg
     4561 tcacaagctg gagtacaatt acaattccca caacgtttac atcaccgcag ataaacagaa
     4621 aaatggcatc aaagcgaatt tcaaaatccg tcacaacatt gaggacggtg gtgttcaact
     4681 ggcggatcat taccagcaaa acaccccgat tggtgacggt ccggtcctgt tgccggataa
     4741 ccattatctg tcttaccaaa gcaaactgag caaagatccg aacgagaagc gcgaccacat
     4801 ggtgctgctg gagtttgtga ccgctgccgg tattaccctg ggtatggatg agctgtataa
     4861 ataataagct tctcggtacc aaattccaga aaagaggcct cccgaaaggg gggccttttt
     4921 tcgttttggt cctccttggc gctgcaagaa ttcaagctta gcgccaagga ggaccaaaac
     4981 gaaaaaaggc ccccctttcg ggaggcctct tttctggaat ttggtaccga gaagcttatt
     5041 aattcagttt atgacccagc ttgctaggca agtcgcaata gcgcgcgact gcaacctcgt
     5101 gctgctccac gtaagtctca ttattcgctt ctttaatgcg ttccagacgg tagtcgacat
     5161 agtaaacacc cggcatcttc agatttttcg ccggtttctt gctgcggtac gtcgtcttga
     5221 tatttgcgat caaatggctg ccaccaacca gcttcagtgc catgtcgtta cggccctcca
     5281 ggccaccatc ggccggatac agggtttccg taaacgcctc ccaacccagg gttttcttct
     5341 gcatcaccgg accattgctc gtaaagttga cgccacgaat tttcacgtta tagatcagac
     5401 agccatcttg cagggaggta tcttgggtgg cggtcaacac accaccatct tcatacgtgg
     5461 tgacacgctc ccaggtgaaa ccttccggaa agctctgttt gaagaaatcc ggaatgccct
     5521 gagtatggtt aatgaacgtt ttggaaccgt acaggaagct ggtcgccaga atgtcaaaag
     5581 cgaacggcag tggaccgccc tccacaactt tgatacgcat ggtttgggtg ccttcgtacg
     5641 gcttaccctc accctcagac gtgcacttaa agtggtggtt gtcaacggtg ccttccatgt
     5701 acagcttcat gtgcatgttc tctttgatca gttcgctcat tggttaattt ctcctcttta
     5761 attctagagt agctagcaca atacctagga ctgagctagc tgtcaactcc actatagacc
     5821 gcagcgacgg tctagggacc gctcttggac tcctgttgat agatccagta atgacctcag
     5881 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     5941 atccaggcaa tcaatcgtgc tttcgcgaag gccttgcagg ccaaccagat aagtgaaatc
     6001 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     6061 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     6121 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttgggc
     6181 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6241 cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagg
     6301 ggattcctta aggtatactt tccgctgcat aaccctgctt cggggtcatt atagcgattt
     6361 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
     6421 actttccttg gtgtatccaa cggcgtcagc c
//
