LOCUS       SR_100_015         979 bp DNA     linear   UNA 14-APR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   masha
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        419..437
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    438..462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             477..499
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             518..535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             545..565
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 562"
                     /translation="ENLYFQS"
     gap             965..979
                     /estimated_length=15
ORIGIN
        1 nngacatcgt ataacgttac tggtttcaca ttcnccnccn gaattgactn tnttccgggc
       61 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
      121 tctcccnnat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
      181 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
      241 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
      301 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
      361 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
      421 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      481 tttaacttta agaaggagat ataccatggg cagcagccat caccatcatc accacagcag
      541 cggcgaaaac ctgtattttc agtccgaaga caaaagggcc aaagtaacct ctgctatgca
      601 gaccatgctg ttcacaatgc tgcgcaagct ggataacgac gcgctcaaca acatcatcaa
      661 caatgcacgt gatggttgcg tacctctcaa cattatcccg cttactactg ctgcaaaact
      721 gatggtggtt ataccggact acaacaccta taagaacacc tgtgatggga cgactttcac
      781 ctacgccagc gcactgtggg aaatccagca ggttgtcgat gctgacagta aaatcgttca
      841 gctgagcgag atttctatgg acaattctcc gaacctggcg tggccactga tcgtaactgc
      901 tctgcgtgcg aatagcgcgg tcaaattaca ataaaagctt gcggccgcat aatgctannn
      961 nnncnnnnnn nnnnnnnnn
//
