LOCUS       pAAV-ef1a-dio-asap4e-kv 7121 bp ds-DNA     circular     17-JUL-2023
DEFINITION  .
KEYWORDS    "marker:AmpR, R-ITR, L-ITR"
COMMENT     Imported using the Genbank importer. File name: #36
            paav-ef1a-dio-asap4c 413M-kv (1).gb
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     source          1..1575
                     /label="source:synthetic DNA construct"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1..1815
                     /label="source:synthetic DNA construct"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        156..1419
                     /label="EF-1a"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_fwdcolor="#346ee0"
     primer_bind     390..407
                     /label="pWPTFor"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     1367..1387
                     /label="EF-1aforward"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1450..1483
                     /label="lox2722"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1534..1567
                     /label="loxP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1559..1573
                     /label="INFUSION"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(1579..3093)
                     /label="Translation 3093-1579"
                     /translation="METTVRYEQGSELTKTSSSPTADEPTIKIDDGRDEGNEQDSCSNTIRRKISPFVMSFGFRVFGVVLIIVDIIVVIVDLAISEKKRGIREILEGVSLAIALFFLVDVLMRVFVEGFKNYFRSKLNTLDAVIVVGTLLINMTYSFSDGTPVDGNVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQTVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNTDQMPQMVTLLRVLRIVILIRIMQLASQKKQLEVVTLKGSSGSSGSSTRQSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRSMSSIDSFISCATDFPEATRF*"
     misc_feature    1582..1776
                     /label="Kv 2.1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     variation       1777..1815
                     /label="GSS linker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1816..3093
                     /label="ASAP4c 413M"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3101..3115
                     /label="INFUSION"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     source          3108..7121
                     /label="source:synthetic DNA construct"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          3108..7121
                     /label="source:synthetic DNA construct(1)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3108..3139)
                     /label="lox2722(1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3190..3223)
                     /label="loxP(1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3238..3846
                     /label="WPRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3323..3340)
                     /label="pWPTRev"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_fwdcolor="#4ec02b"
     polyA_signal    3866..4344
                     /label="hGH polyA"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_fwdcolor="#ff3eee"
     misc_feature    4384..4524
                     /label="R-ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      4616..4922
                     /label="f1 Origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5441..6298
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      6449..7116
                     /label="pUC ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg
     1561 aagttatggc gcgcctcatt aaaagcgggt cgcttccgga aaatcggtcg cgcagctaat
     1621 aaagctatca atgctgctca tgctgcgcat atcaatcacg ccttcggtgc gcgccggcag
     1681 cggcgccacc gggctcggca tgctgctcat ttccagttct tccggcggtt tgctctgcgg
     1741 cgccatttct ttggtgttca gaatcggctg gctctgacgc gtggaagatc cagatgaacc
     1801 tgaagaaccc ttaagggtta ccacttcaag ttgtttcttc tgtgaagcca gctgcattat
     1861 tcttattaag ataacaattc tcagaactcg aagaagagta accatctgcg gcatctgatc
     1921 tgtgttatat tccagtttat gccccagaat gttgccatct tctttaaaat cggtgccttt
     1981 cagttcaatg cggttcacca gggtatcgcc ttcaaatttc accaccgcgc gggttttata
     2041 tttgccatca tctttaaagc taatggtgcg ttcctgcaca tagccttccg gcatcgcgct
     2101 tttaaaaaaa tcatggcgtt tcatatgatc cggatagcgg ctaaagcact gcacgccata
     2161 ggttaaggtg gtcaccaggg tcggccacgg caccggtagt ttgccggtgg tgcaaataaa
     2221 tttcagggtc agtttgccaa tggtcgcatc gccttcgcct tcgccgcgca cgctaaattt
     2281 atggccgttc acatcgccat ccagttccac cagaatcggc accacgccgg taaacagttc
     2341 ttcgccctgg ctcgcgctgc cgccggtgcc gccatacagt tcatccatgc cgtgtgtaat
     2401 gcccgctgcg gtcacaaatt ccagcagcac catgtgatcg cgtttttcgt tcggatcttt
     2461 gctcagcacg gtctggtggc tcagataatg gttatccggc agcagcaccg ggccatcgcc
     2521 aatcggggtg ttctgctgat aatgatccgc cagctgcacg ctgccatctt ccacgttatg
     2581 gcgcacggta aagttcgctt taatgccgtt tttctgttta tccgcggtaa tatacacgtt
     2641 accatcaacg ggtgtaccgt cagagaagga gtaggtcata ttaattagca gagtgcccac
     2701 tactatgact gcatccaaag tattcagttt ggaccggaaa tagttcttga agccttcaac
     2761 aaacactctc atgagaacat caacaaggaa gaagagtgct atagccaggg aaacaccttc
     2821 aagaatctct ctaatgcctc ttttcttctc actgatggcc agatccacaa tcaccactat
     2881 gatgtctaca atgataagca caactccaaa tactctgaat ccaaatgaca tcacaaacgg
     2941 ggaaattttt ctcctaatgg tattggaaca gctgtcttgt tcattaccct catcacgacc
     3001 atcatcaatc tttatcgtgg gctcatctgc tgttggagag ctcgaagttt tagtgagctc
     3061 tgacccctgt tcatacctca cagtcgtctc catGGTGGCG CTAGCATaac ttcgtataaa
     3121 gtatcctata cgaagttatt tgccttaacc cagaaattat cactgttatt ctttagaatg
     3181 gtgcaaagaa taacttcgta taatgtatgc tatacgaagt tatgaattcg atatcaagct
     3241 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3661 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3841 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3901 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3961 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     4021 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     4081 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     4141 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     4201 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     4261 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     4321 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4381 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4441 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4501 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4561 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4621 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4681 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4741 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4801 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4861 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4921 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4981 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5041 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5101 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5161 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5221 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5281 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5341 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5401 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5461 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5521 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5581 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5641 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5701 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5761 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5821 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5881 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5941 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6001 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6061 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6121 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6181 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6241 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6301 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6361 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6421 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6481 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6541 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6601 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6661 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6721 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6781 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6841 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6901 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7021 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7081 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//