LOCUS       Exported                4975 bp ds-DNA     circular SYN 17-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTrc-CysGA-Im7 F84A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..492
                     /codon_start=1
                     /label=CysGA-N
                     /translation="METTEQLINEPLDHRGEVVLVGAGPGDAGLLTLKGLQQIQQADVV
                     VYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDP
                     FIFGRGGEELETLCNAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLITGHLKTG
                     G"
     CDS             493..510
                     /codon_start=1
                     /label=Linker-N
                     /translation="SSGSSG"
     CDS             511..765
                     /codon_start=1
                     /label=Im7 F84A
                     /translation="ELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKITE
                     HPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGAKQ"
     CDS             766..795
                     /codon_start=1
                     /label=Linker-C
                     /translation="GSGGSGGGGS"
     CDS             796..1077
                     /codon_start=1
                     /label=CysGA-C
                     /translation="ELDWENLAAEKQTLVFYMGLNQAATIQQKLIEHGMPGEMPVAIVE
                     NGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDKLNWFSNH"
     rep_origin      1080..1535
                     /direction=RIGHT
                     /label=f1 ori
     CDS             1667..2527
                     /codon_start=1
                     /label=bla
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2676..3336
                     /direction=RIGHT
                     /label=pBR322 ori
     operon          complement(3539..4620)
                     /label=lacI
     promoter        4902..4961
                     /label=pTrc
ORIGIN
        1 atggaaacga ccgaacagtt aatcaacgaa ccgctcgacc atcgcggtga agtggtgctg
       61 gttggtgcag gtccgggcga tgccgggctg ctgacactga aaggactgca acaaattcag
      121 caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa tctggtacgc
      181 cgcgatgcgg accgtgtttt cgtcggcaaa cgcgcgggat accactgcgt accccaggaa
      241 gagattaacc agatcctgct gcgggaagcg caaaaaggca aacgcgtggt gcggctgaaa
      301 ggtggcgatc cgtttatttt tggccgtggt ggcgaagagc tggaaacact gtgcaacgcg
      361 ggtattccgt tctcggtggt tccgggtatt accgcagctt ctggttgctc tgcctattcg
      421 ggtattccac tcacgcatcg cgattatgcc cagagcgtac gcttaattac cggacactta
      481 aaaaccggtg gctcatctgg ctcgagcggt gaactgaaaa atagtattag tgattacaca
      541 gaggctgagt ttgttcaact tcttaaggaa attgaaaaag agaatgttgc tgcaactgat
      601 gatgtgttag atgtgttact cgaacacttt gtaaaaatta ctgagcatcc agatggaacg
      661 gatctgatct attatcctag tgataataga gacgatagcc ccgaagggat tgtcaaggaa
      721 attaaagaat ggcgagctgc taacggtaag ccaggagcta aacagggatc cggtggttct
      781 ggtggtggtg gttctgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg
      841 ttctatatgg ggttgaatca ggccgcgact attcagcaaa agctgattga acacggaatg
      901 ccaggcgaaa tgccggtggc aattgtcgaa aacggtacgg cagtcacgca gcgcgtgatt
      961 gacggtacgc tcacacagct gggagaactg gcgcagcaaa tgaacagtcc atcgctaatt
     1021 attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa ccattaacca
     1081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1201 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     1261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1501 cgcgaatttt aacaaaatat taacgtttac aatttcaggt ggcacttttc ggggaaatgt
     1561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1681 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1741 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1801 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1861 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1921 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1981 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2041 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2101 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2161 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2221 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2281 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2341 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2641 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2821 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2881 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2941 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3001 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3061 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3121 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3181 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3241 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3301 ggccttttta cggttcctgg ccttttgctg gccttttgct cacccggtta ctggaacgtt
     3361 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     3421 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     3481 cgatgcagat ccggaattcg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3541 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3601 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     3661 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     3721 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac
     3781 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     3841 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     3901 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     3961 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     4021 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     4081 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     4141 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     4201 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     4261 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     4321 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     4381 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     4441 actgtttgcc cgccagttgt ttgccacgcg gttgggaatg taattcagct ccgccatcgc
     4501 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
     4561 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
     4621 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     4681 gcgccattcg atggtgtcgg aattctcatg tttgacagct tatcatcgac tgcacggtgc
     4741 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg attggctgtg caggtcgtaa
     4801 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
     4861 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatccgg
     4921 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agcat
//
