LOCUS       Exported               10157 bp ds-DNA     circular SYN 21-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    plex307-3xflag-ccdb-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 21, 2023 from SnapGene Viewer 5.1.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(114..492)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(677..781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(807..1262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     1404..1420
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1430..1448
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      1469..1604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1644..1765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             1843..2076
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2148..2736
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2254..2265
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2787..2828)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDTT"
     protein_bind    2836..2960
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3001..3306)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKDAINLMFWGI"
     CDS             complement(3626..4306)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     promoter        complement(4360..4390)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(4415..4539)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4564..4629)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      4629..4638
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(4652..5830)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4661..5599
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    5968..6085
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6128..6730)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     promoter        complement(6762..6958)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(6770..6980)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6776..6911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7211..7255)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7440..7673
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8166..8291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8338..8518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8519..8745)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        complement(8773..8791)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8812..8828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8836..8852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8860..8890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8905..8926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9214..9802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
       61 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
      361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      781 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
      841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     1021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     1081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     1141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     1201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     1261 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1321 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1381 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     1441 actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg
     1501 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     1561 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     1621 gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1681 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1741 cattataagc tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt
     1801 ataaatactg aataataaga tgacatgaac tactatacgt actgctagag attttccaca
     1861 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     1921 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     1981 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca
     2041 gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
     2101 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa agtcgacgcg gggaggcggc
     2161 ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc
     2221 cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa
     2281 ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga
     2341 tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg
     2401 ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc
     2461 ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac
     2521 agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa
     2581 ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa
     2641 aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt
     2701 caatctttca caaattttgt aatccagagg ttgattgtcg acttaacgcg ttgattgtcg
     2761 acttaaccgg tactagttca ttactacgta gtatcgagac cgaggagagg gttagggata
     2821 ggcttaccga tatcaaccac tttgtacaag aaagctgaac gagaaacgta aaatgatata
     2881 aatatcaata tattaaatta gattttgcat aaaaaacaga ccacataata ctgtaaaaca
     2941 caacatatcc agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat
     3001 ttatattccc cagaacatca ggttaatggc gtctttgatg tcattttcgc ggtggctgag
     3061 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     3121 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacggggga ctttatctga
     3181 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     3241 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     3301 ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
     3361 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
     3421 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
     3481 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt
     3541 tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc
     3601 aaatacgcat actgttatct ggcttttagt aagccggatc ctctagatta cgccccgcct
     3661 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     3721 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     3781 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     3841 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     3901 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     3961 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     4021 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     4081 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     4141 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     4201 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     4261 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     4321 ctcctgaaaa tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat
     4381 aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt
     4441 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     4501 attttacgtt tctcgttcag cttttttgta caaacttgtt tgatgtccac cacctggtcc
     4561 acccttgtca tcgtcatcct tgtaatcgat gtcatgatct ttataatcac cgtcatggtc
     4621 tttgtagtcc atggtggcaa gggtgctagc ctcacgacac ctgaaatgga agaaaaaaac
     4681 tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt
     4741 ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt
     4801 ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc
     4861 tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga
     4921 cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg
     4981 agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg
     5041 ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg
     5101 gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg
     5161 gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt
     5221 cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa
     5281 gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag
     5341 acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg cggcggcccc
     5401 agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc
     5461 tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg tactgcagcc
     5521 aggtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca ggcccgcggg
     5581 aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca
     5641 cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt
     5701 acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc
     5761 cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg
     5821 caccggagcc aattcccact cctttcaaga cctagaaggt ccattagctg caaagattcc
     5881 tctctgttta aaactttatc catctttgca cctgcaggcc tcgagccggc gccaaagtgg
     5941 atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa
     6001 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
     6061 cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgtc tcaagatcta
     6121 gaattcgtta actggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca
     6181 cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg
     6241 cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca
     6301 cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga
     6361 accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg
     6421 ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg
     6481 cttcgacgct ctccggcgtg gtccagaccg ccacggcggc gccgtcgtcc gcgacccaca
     6541 ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct
     6601 cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg
     6661 cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt
     6721 actcggtcat ggtaagctcc ggtggtacct ctagaaagct ttttgcaaaa gcctaggcct
     6781 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     6841 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     6901 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagacgcatg
     6961 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     7021 ttgagatgca atgcatgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     7081 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     7141 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     7201 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     7261 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     7321 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     7381 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     7441 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     7501 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     7561 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     7621 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     7681 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     7741 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     7801 ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc
     7861 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     7921 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     7981 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     8041 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     8101 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     8161 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     8221 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     8281 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     8341 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     8401 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     8461 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccgt
     8521 ttatgtatcg agctaggcac ttaaatacaa tatctctgca atgcggcaat tcagtggttc
     8581 gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa ggcacgatcg taccacctta
     8641 cttccaccaa tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca
     8701 tcgttaccat gttgcaagac tacaagagta ttgcataaga ctacattaag cttgcagctc
     8761 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     8821 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8881 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8941 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9001 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10141 atcagcaata aaccagc
//
