LOCUS       Exported                4880 bp ds-DNA     circular SYN 20-AUG-2022
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..562
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence"
                     /note="color: #ffc0cb"
     source          602..1315
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(3)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(3)"
                     /note="color: #ffc0cb"
     source          1316..1705
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(1)
                     /note="/locus_tag=source:Sequence(1)"
                     /note="color: #ffc0cb"
     source          1706..4880
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(2)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(2)"
                     /note="color: #ffc0cb"
     promoter        5..509
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="color: #346ee0"
     misc_feature    12..299
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="color: #7eff74"
     misc_feature    466..486
                     /locus_tag="CMV_fwd_primer"
                     /label=CMV_fwd_primer
                     /note="color: #7eff74"
     promoter        467..536
                     /locus_tag="CMV_promoter"
                     /label=CMV_promoter
                     /note="color: #346ee0"
     promoter        510..562
                     /locus_tag="CMV_immearly_promoter(1)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(1)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     CDS             560..562
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /note="color: #e9d024"
                     /translation="M"
     misc_feature    563..601
                     /locus_tag="SV40A5"
                     /label=SV40A5
                     /note="color: #00ffff"
     gene            602..1315
                     /gene="EGFP"
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             602..1315
                     /codon_start=1
                     /locus_tag="ORF frame 2(2)"
                     /label=ORF frame 2
                     /label=ORF frame 2(2)
                     /note="color: #e9d024"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        602..1315
                     /locus_tag="CMV_immearly_promoter(3)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(3)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     misc_feature    complement(644..665)
                     /locus_tag="EGFP_N_primer"
                     /label=EGFP_N_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    782..811
                     /locus_tag="Y66 (EGFP)"
                     /label=Y66 (EGFP)
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     misc_feature    1252..1273
                     /locus_tag="EGFP_C_primer"
                     /label=EGFP_C_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     misc_feature    1316..1705
                     /locus_tag="Insert"
                     /label=Insert
                     /note="color: #00ffff"
     CDS             1316..1498
                     /codon_start=1
                     /locus_tag="ORF frame 1"
                     /label=ORF frame 1
                     /note="color: #e9d024"
                     /translation="MLAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQK
                     LNDSQAPKADAQQNN"
     terminator      1577..1705
                     /locus_tag="T7_terminator"
                     /label=T7_terminator
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     *"
                     /note="color: #9d1b1c"
     misc_feature    complement(1619..1637)
                     /locus_tag="T7_Terminal_primer"
                     /label=T7_Terminal_primer
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     *"
                     /note="color: #7eff74; direction: LEFT"
     promoter        1706..4836
                     /locus_tag="CMV_immearly_promoter(2)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(2)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     terminator      1706..1736
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #9d1b1c"
     misc_feature    1706..1725
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     rep_origin      complement(1875..2181)
                     /direction=LEFT
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     promoter        2260..2288
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     misc_feature    complement(2354..2374)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    complement(2360..2575)
                     /locus_tag="SV40_enhancer"
                     /label=SV40_enhancer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        2372..2640
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     rep_origin      2539..2616
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     misc_feature    2601..2620
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     CDS             2723..3517
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /note="color: #e9d024"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2726..3514
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE 
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY 
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             complement(3032..3568)
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /note="color: #e9d024"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPH
                     CISHDGYFLGRSKVR"
     terminator      3692..3961
                     /locus_tag="TK_PA_terminator"
                     /label=TK_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #9d1b1c"
     rep_origin      4109..4728
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #999999"
ORIGIN
        1 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      481 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      541 agcgctaccg gtcgccacca tgggcccaaa aaagaaagcc aaagttggag gcgggggcag
      601 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      661 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      721 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      781 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      841 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      901 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      961 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1021 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1081 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1201 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1261 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct
     1321 tgctcaacaa aatgcttttt atcaagtctt aaatatgcct aacttaaatg ctgatcaacg
     1381 caatggtttt atccaaagcc ttaaagatga tccaagccaa agtgctaacg ttttaggtga
     1441 agctcaaaaa cttaatgact ctcaagctcc aaaagctgat gcgcaacaaa ataactaagt
     1501 cgacccgggt atccgtatga tgtgcctgac tacgcatgat atctcgagca ccaccaccac
     1561 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     1621 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     1681 ctgaaaggag gaactatatc cggatgtggt ttgtccaaac tcatcaatgt atcttaacgc
     1741 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     1801 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     1861 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     1921 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     1981 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2041 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2101 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2161 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     2221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     2341 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     2401 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     2461 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     2521 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2581 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2641 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     2701 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     2761 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     2821 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     2881 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     2941 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3001 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3061 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     3121 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     3181 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     3241 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     3301 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     3361 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     3421 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     3481 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     3541 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     3601 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     3661 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     3721 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     3781 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     3841 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     3901 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     3961 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4801 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     4861 agttcatagc ccatatatgg
//
