LOCUS       Exported                5727 bp ds-DNA     circular SYN 19-APR-2023
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..562
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence"
                     /note="color: #ffc0cb"
     source          602..1315
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(3)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(3)"
                     /note="color: #ffc0cb"
     source          2396..2552
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(1)
                     /note="/locus_tag=source:Sequence(1)"
                     /note="color: #ffc0cb"
     source          2553..5727
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(2)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(2)"
                     /note="color: #ffc0cb"
     promoter        5..509
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="color: #346ee0"
     misc_feature    12..299
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="color: #7eff74"
     misc_feature    466..486
                     /locus_tag="CMV_fwd_primer"
                     /label=CMV_fwd_primer
                     /note="color: #7eff74"
     promoter        467..536
                     /locus_tag="CMV_promoter"
                     /label=CMV_promoter
                     /note="color: #346ee0"
     promoter        510..562
                     /locus_tag="CMV_immearly_promoter(1)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(1)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     CDS             560..562
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /note="color: #e9d024"
                     /translation="M"
     misc_feature    563..601
                     /locus_tag="SV40"
                     /label=SV40
                     /note="color: #00ffff"
     gene            602..1315
                     /gene="EGFP"
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             602..1315
                     /codon_start=1
                     /locus_tag="ORF frame 2(2)"
                     /label=ORF frame 2
                     /label=ORF frame 2(2)
                     /note="color: #e9d024"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        602..1315
                     /locus_tag="CMV_immearly_promoter(3)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(3)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     misc_feature    complement(644..665)
                     /locus_tag="EGFP_N_primer"
                     /label=EGFP_N_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    782..811
                     /locus_tag="Y66 (EGFP)"
                     /label=Y66 (EGFP)
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     misc_feature    1252..1273
                     /locus_tag="EGFP_C_primer"
                     /label=EGFP_C_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     misc_feature    1346..2389
                     /locus_tag="PrA"
                     /label=PrA
                     /note="color: #00ffff"
     misc_feature    1844..1849
                     /locus_tag="New Feature"
                     /label=New Feature
                     /note="color: #fe0101"
     misc_feature    2024..2029
                     /locus_tag="Seam"
                     /label=Seam
                     /note="color: #ff0000"
     misc_feature    2390..2395
                     /locus_tag="seam"
                     /label=seam
                     /note="color: #f90d06"
     misc_feature    2396..2552
                     /locus_tag="Insert"
                     /label=Insert
                     /note="/ApEinfo_hidden="
                     /note="color: #00ffff"
     terminator      2424..2552
                     /locus_tag="T7_terminator"
                     /label=T7_terminator
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     FNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNK 
                     FNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPK*"
                     /note="color: #9d1b1c"
     misc_feature    complement(2466..2484)
                     /locus_tag="T7_Terminal_primer"
                     /label=T7_Terminal_primer
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     FNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNK 
                     FNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPK*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        2553..5683
                     /locus_tag="CMV_immearly_promoter(2)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(2)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     terminator      2553..2583
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #9d1b1c"
     misc_feature    2553..2572
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     rep_origin      complement(2722..3028)
                     /direction=LEFT
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     promoter        3107..3135
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     misc_feature    complement(3201..3221)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    complement(3207..3422)
                     /locus_tag="SV40_enhancer"
                     /label=SV40_enhancer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        3219..3487
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     rep_origin      3386..3463
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     misc_feature    3448..3467
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     CDS             3570..4364
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /note="color: #e9d024"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3573..4361
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE 
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY 
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             complement(3879..4415)
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /note="color: #e9d024"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPH
                     CISHDGYFLGRSKVR"
     terminator      4539..4808
                     /locus_tag="TK_PA_terminator"
                     /label=TK_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #9d1b1c"
     rep_origin      4956..5575
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #999999"
ORIGIN
        1 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      481 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      541 agcgctaccg gtcgccacca tgggcccaaa aaagaaaaga aaagttggag gcgggggcag
      601 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      661 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      721 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      781 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      841 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      901 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      961 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1021 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1081 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1201 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1261 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct
     1321 tgcacagcaa aacgctttct atcaagtact aaacatgcca aacttgaatg cagatcaacg
     1381 taacggtttc atccaaagtt tgaaggatga tcctagtcaa tctgccaacg tgttgggtga
     1441 agctcaaaag ttgaatgaca gtcaagcacc aaaggcagat gctcagcaaa ataactttaa
     1501 caaagatcag caatctgcat tctacgagat tcttaatatg cctaatttaa atgaggcgca
     1561 acgtaatggt tttattcaaa gccttaagga tgatccatct caatccacaa acgtcctggg
     1621 agaagcaaag aaactaaatg aatcacaagc ccctaaggca gataacaact ttaataaaga
     1681 acaacaaaac gcattctatg aaatcctaaa catgcctaat ctgaacgagg aacaaaggaa
     1741 tggctttata caatccttaa aggatgaccc ttcccagtct gctaatttat tatcagaggc
     1801 taagaagtta aacgaatctc aggctccaaa ggccgacaat aagacgcgtt ttaacaagga
     1861 gcaacagaac gctttttatg aaatcttgca tttacccaac ttgaacgaag agcaaagaaa
     1921 tggttttata caatctttaa aggatgatcc atcacaaagt gctaaccttt tagctgaagc
     1981 taaaaaatta aatgatgcac aagctccaaa ggcagataac aaagtcgact ttaataaggc
     2041 acagcaaaac gctttctatc aagtcttaaa catgccaaat ctgaatgctg atcaaagaaa
     2101 cggtttcatc caatctttga aagacgatcc tagccagtcc gcaaatgtac ttggagaagc
     2161 gcaaaaactg aacgatagcc aagctcctaa agcagatgca caacaaaaca attttaataa
     2221 agatcaacaa tcagctttct acgaaattct aaacatgcca aacctgaatg aagcacaaag
     2281 aaacgggttt atccaatcct taaaagatga cccaagccaa tctacgaatg ttttgggaga
     2341 agctaagaaa cttaatgaat ctcaagcacc aaaggccgac aacaattaac tcgagcacca
     2401 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     2461 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     2521 ttttttgctg aaaggaggaa ctatatccgg atgtggtttg tccaaactca tcaatgtatc
     2581 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     2641 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     2701 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     2761 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     2821 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     2881 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     2941 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3001 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     3061 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     3181 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3361 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3421 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     3541 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     3601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3721 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     3781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     3961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4081 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     4381 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     4441 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4501 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     4561 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     4621 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     4681 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     4741 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     4801 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     4861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5641 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     5701 ggtcattagt tcatagccca tatatgg
//
