LOCUS       Exported                4604 bp ds-DNA     circular SYN 20-APR-2020
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..559
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence"
                     /note="color: #ffc0cb"
     source          560..562
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(1)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(1)"
                     /note="color: #ffc0cb"
     source          1430..4604
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(2)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(2)"
                     /note="color: #ffc0cb"
     promoter        5..509
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="color: #346ee0"
     misc_feature    12..299
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="color: #7eff74"
     misc_feature    466..486
                     /locus_tag="CMV_fwd_primer"
                     /label=CMV_fwd_primer
                     /note="color: #7eff74"
     promoter        467..536
                     /locus_tag="CMV_promoter"
                     /label=CMV_promoter
                     /note="color: #346ee0"
     promoter        510..559
                     /locus_tag="CMV_immearly_promoter(2)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(2)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     CDS             560..562
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /note="color: #e9d024"
                     /translation="M"
     promoter        560..562
                     /locus_tag="CMV_immearly_promoter(1)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(1)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     CDS             563..604
                     /locus_tag="V5"
                     /label=V5
                     /note="color: #808080; direction: RIGHT"
     misc_feature    complement(605..1222)
                     /locus_tag="Twist1 murine"
                     /label=Twist1 murine
                     /note="color: #00ffff; direction: LEFT"
     terminator      1301..1429
                     /locus_tag="T7_terminator"
                     /label=T7_terminator
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     *"
                     /note="color: #9d1b1c"
     misc_feature    complement(1343..1361)
                     /locus_tag="T7_Terminal_primer"
                     /label=T7_Terminal_primer
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     *"
                     /note="color: #7eff74; direction: LEFT"
     promoter        1430..4560
                     /locus_tag="CMV_immearly_promoter(3)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(3)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     terminator      1430..1460
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #9d1b1c"
     misc_feature    1430..1449
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     rep_origin      complement(1599..1905)
                     /direction=LEFT
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     promoter        1984..2012
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     misc_feature    complement(2078..2098)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    complement(2084..2299)
                     /locus_tag="SV40_enhancer"
                     /label=SV40_enhancer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        2096..2364
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     rep_origin      2263..2340
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     misc_feature    2325..2344
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     CDS             2447..3241
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /note="color: #e9d024"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2450..3238
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE 
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY 
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             complement(2756..3292)
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /note="color: #e9d024"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPH
                     CISHDGYFLGRSKVR"
     terminator      3416..3685
                     /locus_tag="TK_PA_terminator"
                     /label=TK_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #9d1b1c"
     rep_origin      3833..4452
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #999999"
ORIGIN
        1 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      481 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      541 agcgctaccg gtcgccacca tgggaaagcc tattcctaat cctcttctag gtctagattc
      601 tactatgcag gacgtgtcca gctcgccagt ctctccggcc gacgacagcc tgagcaacag
      661 cgaggaggag ccggaccggc agcagccggc gagcggcaag cgcggggctc gcaagagacg
      721 cagcagtcgg cgcagcgcgg gcggcagcgc ggggcccggc ggggccacgg gcgggggcat
      781 cggaggcggc gacgagccag gcagcccggc ccagggcaag cgcggcaaga aatctgcggg
      841 cggaggcggc ggcggcggcg cgggcggagg tggtggcggc ggcggcggca gcagcagcgg
      901 gggcgggagc ccgcagtcgt acgaggagct gcagacccag cgggtcatgg ctaacgtgcg
      961 ggagcgccag cgcacgcagt cgctgaacga ggcgttcgcc gccctgcgca agatcatccc
     1021 cacgctgccc tcggacaagc tgagcaagat tcagaccctc aaactggcgg ccaggtacat
     1081 cgacttcctg taccaggtcc tgcagagcga cgagctggac tccaagatgg caagctgcag
     1141 ctatgtggcc cacgagcggc tcagctacgc cttctccgtc tggaggatgg agggggcctg
     1201 gtccatgtcc gcgtcccact aggtcgaccc gggtatccgt atgatgtgcc tgactacgca
     1261 tgatatctcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
     1321 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     1381 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatg tggtttgtcc
     1441 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     1501 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     1561 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     1621 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     1681 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     1741 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     1801 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     1861 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     1921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2041 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2221 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2341 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2401 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     2461 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     2521 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     2581 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     2641 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     2701 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     2761 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     2821 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     2881 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     2941 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     3001 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     3061 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     3121 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     3181 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     3241 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     3301 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     3361 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     3421 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     3481 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     3541 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     3601 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     3661 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     3721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3961 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
     4561 atagtaatca attacggggt cattagttca tagcccatat atgg
//
