LOCUS       Exported                4628 bp ds-DNA     circular SYN 04-AUG-2020
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..559
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence"
                     /note="color: #ffc0cb"
     source          560..562
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(1)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(1)"
                     /note="color: #ffc0cb"
     source          1454..4628
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(2)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(2)"
                     /note="color: #ffc0cb"
     promoter        5..509
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="color: #346ee0"
     misc_feature    12..299
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="color: #7eff74"
     misc_feature    466..486
                     /locus_tag="CMV_fwd_primer"
                     /label=CMV_fwd_primer
                     /note="color: #7eff74"
     promoter        467..536
                     /locus_tag="CMV_promoter"
                     /label=CMV_promoter
                     /note="color: #346ee0"
     promoter        510..559
                     /locus_tag="CMV_immearly_promoter(2)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(2)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     CDS             560..562
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /note="color: #e9d024"
                     /translation="M"
     promoter        560..562
                     /locus_tag="CMV_immearly_promoter(1)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(1)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     misc_feature    563..586
                     /locus_tag="SV40A5"
                     /label=SV40A5
                     /note="color: #ffc600"
     CDS             587..628
                     /locus_tag="V5"
                     /label=V5
                     /note="color: #808080; direction: RIGHT"
     misc_feature    complement(629..842)
                     /locus_tag="Twist1 murine"
                     /label=Twist1 murine
                     /note="color: #00ffff; direction: LEFT"
     misc_feature    738
                     /locus_tag="K38R"
                     /label=K38R
                     /note="color: #ff0000"
     misc_feature    complement(843)
                     /locus_tag="Twist1 murine(1)"
                     /label=Twist1 murine
                     /label=Twist1 murine(1)
                     /note="color: #00ffff; direction: LEFT"
     misc_feature    843
                     /locus_tag="K73R"
                     /label=K73R
                     /note="color: #ff0000"
     misc_feature    complement(844..1246)
                     /locus_tag="Twist1 murine(2)"
                     /label=Twist1 murine
                     /label=Twist1 murine(2)
                     /note="color: #00ffff; direction: LEFT"
     terminator      1325..1453
                     /locus_tag="T7_terminator"
                     /label=T7_terminator
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     *"
                     /note="color: #9d1b1c"
     misc_feature    complement(1367..1385)
                     /locus_tag="T7_Terminal_primer"
                     /label=T7_Terminal_primer
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     *"
                     /note="color: #7eff74; direction: LEFT"
     promoter        1454..4584
                     /locus_tag="CMV_immearly_promoter(3)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(3)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     terminator      1454..1484
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #9d1b1c"
     misc_feature    1454..1473
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     rep_origin      complement(1623..1929)
                     /direction=LEFT
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     promoter        2008..2036
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     misc_feature    complement(2102..2122)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    complement(2108..2323)
                     /locus_tag="SV40_enhancer"
                     /label=SV40_enhancer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        2120..2388
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     rep_origin      2287..2364
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     misc_feature    2349..2368
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     CDS             2471..3265
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /note="color: #e9d024"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2474..3262
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE 
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY 
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             complement(2780..3316)
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /note="color: #e9d024"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPH
                     CISHDGYFLGRSKVR"
     terminator      3440..3709
                     /locus_tag="TK_PA_terminator"
                     /label=TK_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #9d1b1c"
     rep_origin      3857..4476
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #999999"
ORIGIN
        1 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      481 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      541 agcgctaccg gtcgccacca tgggcccaaa aaagaaagcc aaagttggaa agcctattcc
      601 taatcctctt ctaggtctag attctactat gcaggacgtg tccagctcgc cagtctctcc
      661 ggccgacgac agcctgagca acagcgagga ggagccggac cggcagcagc cggcgagcgg
      721 caagcgcggg gctcgcagga gacgcagcag tcggcgcagc gcgggcggca gcgcggggcc
      781 cggcggggcc acgggcgggg gcatcggagg cggcgacgag ccaggcagcc cggcccaggg
      841 caggcgcggc aagaaatctg cgggcggagg cggcggcggc ggcgcgggcg gaggtggtgg
      901 cggcggcggc ggcagcagca gcgggggcgg gagcccgcag tcgtacgagg agctgcagac
      961 ccagcgggtc atggctaacg tgcgggagcg ccagcgcacg cagtcgctga acgaggcgtt
     1021 cgccgccctg cgcaagatca tccccacgct gccctcggac aagctgagca agattcagac
     1081 cctcaaactg gcggccaggt acatcgactt cctgtaccag gtcctgcaga gcgacgagct
     1141 ggactccaag atggcaagct gcagctatgt ggcccacgag cggctcagct acgccttctc
     1201 cgtctggagg atggaggggg cctggtccat gtccgcgtcc cactaggtcg acccgggtat
     1261 ccgtatgatg tgcctgacta cgcatgatat ctcgagcacc accaccacca ccactgagat
     1321 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     1381 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     1441 actatatccg gatgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     1501 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     1561 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     1621 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     1681 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     1741 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     1801 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     1861 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     1921 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     1981 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2041 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     2101 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2161 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     2221 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2281 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2341 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     2401 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     2461 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     2521 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     2581 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     2641 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     2701 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     2761 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     2821 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     2881 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     2941 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     3001 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     3061 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     3121 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     3181 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     3241 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     3301 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     3361 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     3421 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     3481 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     3541 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     3601 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     3661 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     3721 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3781 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3841 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3901 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3961 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4021 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4081 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4141 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4201 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4261 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4321 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4381 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4441 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4501 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4561 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4621 atatatgg
//
