LOCUS       Exported                6030 bp ds-DNA     circular SYN 13-JAN-2020
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAF 
            YEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNL 
            NEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFI 
            QSLKDDPSQSANLLAEAKKLNDAQAPK*
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..562
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence"
                     /note="color: #ffc0cb"
     source          602..1315
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(6)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(6)"
                     /note="color: #ffc0cb"
     source          1316..1321
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(1)
                     /note="/locus_tag=source:Sequence(1)"
                     /note="color: #ffc0cb"
     source          1322..2005
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(3)
                     /note="/locus_tag=source:Sequence(3)"
                     /note="color: #ffc0cb"
     source          2006..2689
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(2)
                     /note="/locus_tag=source:Sequence(2)"
                     /note="color: #ffc0cb"
     source          2690..2855
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(5)
                     /note="/locus_tag=source:Sequence(5)"
                     /note="color: #ffc0cb"
     source          2856..6030
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /label=source:Sequence
                     /label=source:Sequence(4)
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:Sequence(4)"
                     /note="color: #ffc0cb"
     promoter        5..509
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="color: #346ee0"
     misc_feature    12..299
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="color: #7eff74"
     misc_feature    466..486
                     /locus_tag="CMV_fwd_primer"
                     /label=CMV_fwd_primer
                     /note="color: #7eff74"
     promoter        467..536
                     /locus_tag="CMV_promoter"
                     /label=CMV_promoter
                     /note="color: #346ee0"
     promoter        510..562
                     /locus_tag="CMV_immearly_promoter(1)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(1)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     CDS             560..562
                     /codon_start=1
                     /locus_tag="ORF frame 2"
                     /label=ORF frame 2
                     /note="color: #e9d024"
                     /translation="M"
     misc_feature    563..601
                     /locus_tag="SV40"
                     /label=SV40
                     /note="color: #00ffff"
     gene            602..1315
                     /gene="EGFP"
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             602..1315
                     /codon_start=1
                     /locus_tag="ORF frame 2(2)"
                     /label=ORF frame 2
                     /label=ORF frame 2(2)
                     /note="color: #e9d024"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        602..1315
                     /locus_tag="CMV_immearly_promoter(3)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(3)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     misc_feature    complement(644..665)
                     /locus_tag="EGFP_N_primer"
                     /label=EGFP_N_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    782..811
                     /locus_tag="Y66 (EGFP)"
                     /label=Y66 (EGFP)
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     misc_feature    1252..1273
                     /locus_tag="EGFP_C_primer"
                     /label=EGFP_C_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     CDS             1316..1321
                     /codon_start=1
                     /locus_tag="ORF frame 1"
                     /label=ORF frame 1
                     /note="color: #e9d024"
                     /translation="ML"
     misc_feature    1316..1321
                     /locus_tag="Insert"
                     /label=Insert
                     /note="/ApEinfo_hidden="
                     /note="color: #00ffff"
     misc_feature    1316..1321
                     /locus_tag="New Feature"
                     /label=New Feature
                     /note="color: #00ffff"
     CDS             1322..2005
                     /codon_start=1
                     /locus_tag="ORF frame 1(2)"
                     /label=ORF frame 1
                     /label=ORF frame 1(2)
                     /note="color: #e9d024"
                     /translation="MQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLN
                     DSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKK
                     LNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKL
                     NESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLN
                     DAQAPK"
     misc_feature    1322..2005
                     /locus_tag="Insert(2)"
                     /label=Insert
                     /label=Insert(2)
                     /note="/ApEinfo_hidden="
                     /note="color: #00ffff"
     CDS             1322..2005
                     /locus_tag="4PrA"
                     /label=4PrA
                     /note="color: #f1e278; direction: RIGHT"
     CDS             2006..2689
                     /codon_start=1
                     /locus_tag="ORF frame 1(1)"
                     /label=ORF frame 1
                     /label=ORF frame 1(1)
                     /note="color: #e9d024"
                     /translation="MQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLN
                     DSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKK
                     LNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKL
                     NESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLN
                     DAQAPK"
     misc_feature    2006..2689
                     /locus_tag="Insert(1)"
                     /label=Insert
                     /label=Insert(1)
                     /note="/ApEinfo_hidden="
                     /note="color: #00ffff"
     CDS             2006..2689
                     /locus_tag="4PrA(1)"
                     /label=4PrA
                     /label=4PrA(1)
                     /note="color: #f1b878; direction: RIGHT"
     misc_feature    2690..2855
                     /locus_tag="Insert(3)"
                     /label=Insert
                     /label=Insert(3)
                     /note="/ApEinfo_hidden="
                     /note="color: #00ffff"
     CDS             2690..2692
                     /codon_start=1
                     /locus_tag="ORF frame 1(3)"
                     /label=ORF frame 1
                     /label=ORF frame 1(3)
                     /note="color: #e9d024"
                     /translation="M"
     misc_feature    2693..2698
                     /locus_tag="New Feature(1)"
                     /label=New Feature
                     /label=New Feature(1)
                     /note="color: #00ffff"
     terminator      2727..2855
                     /locus_tag="T7_terminator"
                     /label=T7_terminator
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     FNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNK 
                     FNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPK*"
                     /note="color: #9d1b1c"
     misc_feature    complement(2769..2787)
                     /locus_tag="T7_Terminal_primer"
                     /label=T7_Terminal_primer
                     /note="/translation=MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMSK
                     GEELF 
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGY 
                     GVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE 
                     LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY 
                     QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKKL 
                     AQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNN 
                     FNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNN 
                     FNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNK 
                     FNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPK*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        2856..5986
                     /locus_tag="CMV_immearly_promoter(2)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(2)
                     /note="/ApEinfo_hidden="
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #346ee0"
     terminator      2856..2886
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #9d1b1c"
     misc_feature    2856..2875
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     rep_origin      complement(3025..3331)
                     /direction=LEFT
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     promoter        3410..3438
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     misc_feature    complement(3504..3524)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     misc_feature    complement(3510..3725)
                     /locus_tag="SV40_enhancer"
                     /label=SV40_enhancer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74; direction: LEFT"
     promoter        3522..3790
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #346ee0"
     rep_origin      3689..3766
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #999999"
     misc_feature    3751..3770
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/translation=MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
                     YGKLT 
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN 
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     MVLLEFVTAAGITLGMDELYKSGLRSAKLNESHRLHQFFRDMDDEESWIKEKKLLVSS 
                     EDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQ 
                     FVDHWKELKQLAAARGQRLE*"
                     /note="color: #7eff74"
     CDS             3873..4667
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /note="color: #e9d024"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3876..4664
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="/translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGR 
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL 
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE 
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY 
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /note="color: #ff797d; direction: RIGHT"
     CDS             complement(4182..4718)
                     /codon_start=1
                     /locus_tag="ORF frame 2(1)"
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /note="color: #e9d024"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPH
                     CISHDGYFLGRSKVR"
     terminator      4842..5111
                     /locus_tag="TK_PA_terminator"
                     /label=TK_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #9d1b1c"
     rep_origin      5259..5878
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
                     /note="color: #999999"
ORIGIN
        1 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      481 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      541 agcgctaccg gtcgccacca tgggcccaaa aaagaaaaga aaagttggag gcgggggcag
      601 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      661 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      721 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      781 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      841 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      901 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      961 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1021 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1081 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1201 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1261 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagct
     1321 tgctcaacaa aatgcttttt atcaagtctt aaatatgcct aacttaaatg ctgatcaacg
     1381 caatggtttt atccaaagcc ttaaagatga tccaagccaa agtgctaacg ttttaggtga
     1441 agctcaaaaa cttaatgact ctcaagctcc aaaagctgat gcgcaacaaa ataacttcaa
     1501 caaagatcaa caaagcgcct tctatgaaat cttgaacatg cctaacttaa acgaagcgca
     1561 acgtaacggc ttcattcaaa gtcttaaaga cgacccaagc caaagcacta acgttttagg
     1621 tgaagctaaa aaattaaacg aatctcaagc accgaaagct gataacaatt tcaacaaaga
     1681 acaacaaaat gctttctatg aaatcttgaa tatgcctaac ttaaacgaag aacaacgcaa
     1741 tggtttcatc caaagcttaa aagatgaccc aagccaaagt gctaacctat tgtcagaagc
     1801 taaaaagtta aatgaatctc aagcaccgaa agcggataac aaattcaaca aagaacaaca
     1861 aaatgctttc tatgaaatct tacatttacc taacttaaac gaagaacaac gcaatggttt
     1921 catccaaagc ctaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     1981 gctaaatgat gctcaagcac caaaagctca acaaaatgct ttttatcaag tcttaaatat
     2041 gcctaactta aatgctgatc aacgcaatgg ttttatccaa agccttaaag atgatccaag
     2101 ccaaagtgct aacgttttag gtgaagctca aaaacttaat gactctcaag ctccaaaagc
     2161 tgatgcgcaa caaaataact tcaacaaaga tcaacaaagc gccttctatg aaatcttgaa
     2221 catgcctaac ttaaacgaag cgcaacgtaa cggcttcatt caaagtctta aagacgaccc
     2281 aagccaaagc actaacgttt taggtgaagc taaaaaatta aacgaatctc aagcaccgaa
     2341 agctgataac aatttcaaca aagaacaaca aaatgctttc tatgaaatct tgaatatgcc
     2401 taacttaaac gaagaacaac gcaatggttt catccaaagc ttaaaagatg acccaagcca
     2461 aagtgctaac ctattgtcag aagctaaaaa gttaaatgaa tctcaagcac cgaaagcgga
     2521 taacaaattc aacaaagaac aacaaaatgc tttctatgaa atcttacatt tacctaactt
     2581 aaacgaagaa caacgcaatg gtttcatcca aagcctaaaa gatgacccaa gccaaagcgc
     2641 taacctttta gcagaagcta aaaagctaaa tgatgctcaa gcaccaaaat aactcgagca
     2701 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     2761 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     2821 gggttttttg ctgaaaggag gaactatatc cggatgtggt ttgtccaaac tcatcaatgt
     2881 atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2941 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     3001 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3061 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3121 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3181 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3241 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3301 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     3361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     3481 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3841 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3901 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3961 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4021 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     4081 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4141 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4201 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4261 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4321 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4381 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4441 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4501 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4561 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4621 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4681 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4741 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4801 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     4861 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4921 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4981 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     5041 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     5101 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     5161 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5221 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5281 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5341 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5401 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5461 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5521 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5581 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5641 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5701 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5761 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5821 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5881 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5941 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     6001 cggggtcatt agttcatagc ccatatatgg
//
