LOCUS       pJUMP24_T24_pRha        7402 bp DNA     circular SYN 21-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Dan Park
  TITLE     Direct Submission
  JOURNAL   Exported Apr 21, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..6
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      16..209
                     /label=T24
     terminator      147..194
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    253..272
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(356..1294)
                     /codon_start=1
                     /standard_name="rhaR "
                     /label=rhaR
                     /note="rhaR"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFAS
                     DQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKH
                     SYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQL
                     EHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSP
                     FALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTEC
                     GFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     CDS             complement(1278..2114)
                     /codon_start=1
                     /standard_name="rhaS "
                     /label=rhaS
                     /note="rhaS"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     misc_feature    2352..2383
                     /standard_name="PRha"
                     /label=PRha
                     /note="PRhaBAD
                     Geneious type: promoter"
     RBS             2406..2426
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2434..3430
                     /codon_start=1
                     /label=ilvA
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLD
                     NVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVK
                     ALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVI
                     AGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKA
                     ALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDV
                     RAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCE"
     CDS             3437..3724
                     /codon_start=1
                     /label=relE
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLR
                     GMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     CDS             3786..3975
                     /codon_start=1
                     /label=ilvA
                     /translation="TLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGY
                     DCHDETNNPAFRFFLAG"
     misc_feature    4019..4039
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      4073..4167
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             4299..5114
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLHRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            5281..5389
                     /note="incP origin of transfer"
     rep_origin      5483..6071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6125..6958)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     misc_feature    6347
                     /label=A
     misc_feature    6661
                     /label=T
     rep_origin      6972..7323
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      7396..7402
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tatagggcga attggcggga gagtgttcac cgacaaacaa cagataaaac aaaaggccca
       61 gtcttccgac tgagcctttt gttttatttg atgtctggca gttccccgcc gctatctgat
      121 catcaaataa taattttttc atgttgaaaa tctccaaaaa aaaggctcca aaaggagcct
      181 ttaattgtat cggtttatca gcttgctttc tcgaggagtt ttgcaggtgc cttggaacac
      241 ctgcttttcg ctgaattcgc ggccgcttct aggctggcga ttcaggttca tcatgccgtt
      301 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagttttt atgcattaat
      361 ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca
      421 ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca
      481 ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgtcgc agatattgat
      541 tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg
      601 cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc agccgggtaa
      661 tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat
      721 ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg ttagcaaacg
      781 gcacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc
      841 ccatgctacc taagcgccag tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc
      901 cctgccagtc aagattcagc ttcagacgct ccgggcaata aataatattc tgcaaaacca
      961 gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag agatcgccac
     1021 gggtaatgcg ataagggcga tcgttgagta catgcaggcc attaccgcgc cagacaatca
     1081 ccagctcaca aaaatcatgt gtatgttcag caaagacatc ttgcggataa cggtcagcca
     1141 cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg
     1201 ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata
     1261 cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt
     1321 gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag
     1381 cgataggcga tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc
     1441 agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt aagctgccga
     1501 tgtagcgtac gcagtgaaag agaaaattga tccgccacgg catcccaatt cacctcatcg
     1561 gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct gttttccagg
     1621 ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa gatctcgcga
     1681 ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc aaccagctgt
     1741 cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg cccatccagc
     1801 tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg cgagcgatac
     1861 agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc atgatcgcgt
     1921 acgaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaacac atgaataccc
     1981 gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc
     2041 tgcgggagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta
     2101 tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg
     2161 tcaggttctt accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga
     2221 tacagcgtga attttcagga aatgcggtga gcatcacatc accacaattc agcaaattgt
     2281 gaacatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg tcagtaacga
     2341 gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat gaacctaggt acagtgctag
     2401 cgtacttgtt taactttaag aaggagatat acaatggctg actcgcaacc cctgtccggt
     2461 gctccggaag gtgccgaata tttaagagca gtgctgcgcg cgccggttta cgaggcggcg
     2521 caggttacgc cgctacaaaa aatggaaaaa ctgtcgtcgc gtcttgataa cgtcattctg
     2581 gtgaagcgcg aagatcgcca gccagtgcac agctttaagc tgcgcggcgc atacgccatg
     2641 atggcgggcc tgacggaaga acagaaagcg cacggcgtga tcactgcttc tgcgggtaac
     2701 cacgcgcagg gcgtcgcgtt ttcttctgcg cggttaggcg tgaaggccct gatcgttatg
     2761 ccaaccgcca ccgccgacat caaagtcgac gcggtgcgcg gcttcggcgg cgaagtgctg
     2821 ctccacggcg cgaactttga tgaagcgaaa gccaaagcga tcgaactgtc acagcagcag
     2881 gggttcacct gggtgccgcc gttcgaccat ccgatggtga ttgccgggca aggcacgctg
     2941 gcgctggaac tgctccagca ggacgcccat ctcgaccgcg tatttgtgcc agtcggcggc
     3001 ggcggtctgg ctgctggcgt ggcggtgctg atcaaacaac tgatgccgca aatcaaagtg
     3061 atcgccgtag aagcggaaga ctccgcctgc ctgaaagcag cgctggatgc gggtcatccg
     3121 gttgatctgc cgcgcgtagg gctatttgct gaaggcgtag cggtaaaacg catcggtgac
     3181 gaaaccttcc gtttatgcca ggagtatctc gacgacatca tcaccgtcga tagcgatgcg
     3241 atctgtgcgg cgatgaagga tttattcgaa gatgtgcgcg cggtggcgga accctctggc
     3301 gcgctggcgc tggcgggaat gaaaaaatat atcgccctgc acaacattcg cggcgaacgg
     3361 ctggcgcata ttctttccgg tgccaacgtg aacttccacg gcctgcgcta cgtctcagaa
     3421 cgctgcgaac tgggcgatgg cctactttct agacttcgac gaacgagccc tgaaagagtg
     3481 gcggaaatta ggaagtactg tgagggagca gctgaaaaag aaattagtag aagttctaga
     3541 aagtccgcgg atagaagcaa acaagctgcg gggaatgcca gactgctaca aaataaagct
     3601 gagaagcagc ggctatcggc tcgtctatca agtaatagac gaaaaagtag tagtcttcgt
     3661 gataagcgta ggaaaaagag aaaggtcaga agtctactcg gaggccgtaa agcgaatctt
     3721 gtaaaggaac gcctctacag cttcgaattc ccggaatcac cgggcgcgct gctgcgcttc
     3781 ctcaacacgc tgggtacgta ctggaacatt tctttgttcc actatcgcag ccatggcacc
     3841 gactacgggc gcgtactggc ggcgttcgaa cttggcgacc atgaaccgga tttcgaaacc
     3901 cggctgaatg agctgggcta cgattgccac gacgaaacca ataacccggc gttcaggttc
     3961 tttttggcgg gttagcttct gactgagttg cacgctggtc tctcgctaat gtgagacgta
     4021 ctagtagcgg ccgctgcagg gagttgtctt cgaagacttc gctctagtct tggactcctg
     4081 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     4141 cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga tttaaatttg
     4201 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     4261 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt cagcgtgaaa
     4321 cgagctgtag ccgtccgcgt ctgaacagca acatggatgc ggatctgtat ggctataaat
     4381 gggcgcgtga taacgtgggt cagagcggtg cgaccattta tcgtctgtat ggcaaaccgg
     4441 atgcgccgga actgtttctg aaacatggca aaggcagcgt ggcgaacgat gtgaccgatg
     4501 aaatggtgcg tctgaactgg ctgaccgaat ttatgccgct gccgaccatt aaacatttta
     4561 ttcgcacccc ggatgatgcg tggctgctga ccaccgcgat tccgggcaaa accgcgtttc
     4621 aggtgctgga agaatatccg gatagcggcg aaaacattgt ggatgcgctg gccgtgtttc
     4681 tgcatcgtct gcatagcatt ccggtgtgca actgcccgtt taacagcgat cgtgtgtttc
     4741 gtctggccca ggcgcagagc cgtatgaaca acggcctggt ggatgcgagc gattttgatg
     4801 atgaacgtaa cggctggccg gtggaacagg tgtggaaaga aatgcataaa ctgctgccgt
     4861 ttagcccgga tagcgtggtg acccacggcg attttagcct ggataacctg attttcgatg
     4921 aaggcaaact gattggctgc attgatgtgg gccgtgtggg cattgcggat cgttatcagg
     4981 atctggccat tctgtggaac tgcctgggcg aatttagccc gagcctgcaa aaacgtctgt
     5041 ttcagaaata tggcattgat aatccggata tgaacaaact gcaatttcat ctgatgctgg
     5101 atgaattttt ctaataatta attggaccgc ggtccgcgcg ttgtcctttt ccgctgcata
     5161 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
     5221 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
     5281 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
     5341 ttcgcacatg gcggtgctca acgggaatcc tgctctgcga ggctggccgt aggccggccg
     5401 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5461 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5521 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5581 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5641 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5701 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5761 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5821 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5881 aaagcgccac gcttcccgaa gggagaaagg cggacaggca tccggtaagc ggcagggtcg
     5941 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6001 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6061 gcctatggaa aaacgccagc aacgcggccg tgaaaggcag gccggtccgt ggtggccacg
     6121 gcctctaggc cagatccagc ggcatctggg ttagtcgagc gcgggccgct tcccatgtct
     6181 caccagggcg agcctgtttc gcgatctcag catctgaaat cttcccggcc ttgcgcttcg
     6241 ctggggcctt acccaccgcc ttggcgggct tcttcggtcc aaaactgaac aacagatgtg
     6301 tgaccttgcg cccggtcttt cgctgcgccc actccacctg tagcgggctg tgctcgttga
     6361 tctgcgtcac ggctggatca agcactcgca acttgaagtc cttgatcgag ggataccggc
     6421 cttccagttg aaaccacttt cgcagctggt caatttctat ttcgcgctgg ccgatgctgt
     6481 cccattgcat gagcagctcg taaagcctga tcgcgtgggt gctgtccatc ttggccacgt
     6541 cagccaaggc gtatttggtg aactgtttgg tgagttccgt caggtacggc agcatgtctt
     6601 tggtgaacct gagttctaca cggccctcac cctcccggta gatgattgtt tgcacccagc
     6661 cggtaatcat cacactcggt cttttcccct tgccattggg ctcttgggtt aaccggactt
     6721 cccgccgttt caggcgcagg gccgcttctt tgagctggtt gtaggaagat tcgataggga
     6781 cacccgccat cgtcgctatg tcctccgccg tcactgaata catcacttca tcggtgacag
     6841 gctcgctcct cttcacctgg ctaatacagg ccagaacgat ccgctgttcc tgaacactga
     6901 ggcgatacgc ggcctcgacc agggcattgc ttttgtaaac cattgggggt gaggccacgt
     6961 tcgacattcc ttgtgtataa ggggacactg tatctgcgtc ccacaataca acaaatccgt
     7021 ccctttacaa caacaaatcc gtcccttctt aacaacaaat ccgtccctta atggcaacaa
     7081 atccgtccct ttttaaactc tacaggccac ggattacgtg gcctgtagac gtcctaaaag
     7141 gtttaaaagg gaaaaggaag aaaagggtgg aaacgcaaaa aacgcaccac tacgtggccc
     7201 cgttggggcc gcatttgtgc ccctgaaggg gcgggggagg cgtctgggca atccccgttt
     7261 taccagtccc ctatcgccgc ctgagagggc gcaggaagcg agtaatcagg gtatcgaggc
     7321 ggattcaccc ttggcgtcca accagcggca ccagcggcgc ctgagagggg cgcgcccagc
     7381 tgtctagggc ggcggatttg tc
//
