LOCUS       pc-3914                 9036 bp    DNA     circular SYN 10-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     CDS             1..717
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    2989..3451
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             3462..3860
                     /note="pLannotate"
                     /label="BSD"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        7361..7664
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    4018..4139
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(6219..6323)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          7943..8959
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(5358..6218)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4599..5187)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(6655..7110)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             700..2838
                     /note="pLannotate"
                     /label="Tet1 (fragment)"
                     /database="swissprot"
                     /identity="90.6"
                     /match_length="35.5"
                     /fragment="True"
                     /other="CDS"
     promoter        7666..7942
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        7274..7292
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(4173..4362)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="96.4"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(7114..7271)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    6555..6654
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3898..4023
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     CDS             6418..6453
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4150..4167
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="16.7"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4362..4416)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     CDS             3861..3888
                     /note="pLannotate"
                     /label="BleoR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2862..2880)
                     /note="pLannotate"
                     /label="chicken β-actin promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.4"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggagaaaacc tatacttcca aggaagatct cgaggcgatc gcatggaagc tgcaccctgt
      781 gactgtgatg gaggtacaca aaaagaaaaa ggcccatatt atacacacct tggggcagga
      841 ccaagtgtgg ctgctgtcag ggagctcatg gagactaggt ttggccagaa ggggaaggca
      901 atccggattg agaagatagt gttcacgggg aaggaaggga agagctctca gggctgcccg
      961 gtcgccaagt gggtgatcag aagaagtggt cctgaagaga agcttatttg tttggttcgt
     1021 gagcgtgtag accatcactg ttcgacggct gtgatagttg tccttatcct gctgtgggaa
     1081 ggtatccctc gcctgatggc tgaccgcctg tacaaagagc tcactgagaa cttgaggtcc
     1141 tacagcggac atcccacaga ccgaagatgt accctcaaca aaaagcgtac ctgcacctgt
     1201 caaggcatcg acccaaaaac ctgcggagcg tccttctcct ttggctgttc gtggagcatg
     1261 tatttcaacg gctgtcggtt tgggaggagt gaaaacccca gaaaattcag acttgctcca
     1321 aactacccct tacataacta ctataagaga attactggaa tgagttctga aggaagtgac
     1381 gtgaaaaccg ggtggatcat tccagaccgc aagaccctca taagcagaga ggaaaaacag
     1441 cttgaaaaga atttacaaga attggctaca gtattagctc cactttacaa gcagatggct
     1501 ccagttgctt atcaaaatca ggtggaatat gaagaagttg ctggagactg tcgacttgga
     1561 aatgaagagg ggcgtccttt ctctggtgtc acctgttgca tggatttttg tgcccattct
     1621 cacaaggaca ttcacaacat gcacaacgga agcaccgtgg tgtgtacgtt gattcgagca
     1681 gatggccgtg acacaaattg tcccgaggat gaacaactcc acgtcctgcc actataccgg
     1741 cttgcagaca ctgatgaatt tggctccgtg gaagggatga aggccaaaat caaatctggg
     1801 gccatccaag tcaatgggcc aaccaggaag aggcgactac gttttactga gcctgttcct
     1861 cgatgtggga agagggccaa aatgaagcag aaccacaata aatcaggtac tgctggtctc
     1921 agaaggaaga ggatctctgc cagcccaaag ggagccccag gttcacacaa cactaagagc
     1981 ttttcatcag cctcatctac ttctcaccta gtgaaagacg aatctacaga cttctgtccc
     2041 ctgcaggctt cctccgcaga aacatctacc tgtacgtaca gtaaaacagc ctcaggtggg
     2101 tttgcagaaa caagtagtat tctccactgc acaatgcctt ctggagcaca cagtggtgct
     2161 aatgcagctg ctggggaatg tactggaacg gtgcagcctg ccgaggtggc tgctcatcct
     2221 caccagtctc ttcccacagc cgattctccc gttcatgctg agcctctcac tagtccatct
     2281 gagcagctaa cttctaacca gtcaaaccag cagctccctc tcctcagcaa ttctcagaaa
     2341 ctggcttcct gtcaggtgga agatgagcgg caccctgaag cggatgagcc tcagcacccc
     2401 gaggacgata acttgcctca acttgatgaa ttctggtcag acagtgagga gatctacgcc
     2461 gatccttcct ttggtggcgt ggcgatagca cccattcacg gctcggtgct cattgagtgc
     2521 gctcggaagg agcttcatgc taccacctct ttgcgctccc ccaaacgagg ggtccctttt
     2581 cgtgtgtccc ttgtattcta ccagcacaaa agcctaaaca agcctaatca tggttttgat
     2641 atcaacaaaa ttaagtgtaa atgcaaaaaa gtaacgaaaa aaaagcccgc agaccgggag
     2701 tgtcctgatg tatcccccga agccaattta tcacaccaaa ttccttctcg agttgcatca
     2761 accttaaccc gagacaatgt tgttaccgtg tccccatact ctctcactca tgttgcggga
     2821 ccctacaatc gttgggtcta agcggccgca gttaacgaat tccgcccccc cccccccccc
     2881 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     2941 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3001 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3061 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3121 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3181 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3241 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3301 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3361 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     3421 cgtggttttc ctttgaaaaa cacgatgata agcttgccac catggccaag cctttgtctc
     3481 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     3541 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     3601 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     3661 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     3721 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     3781 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     3841 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     3901 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     3961 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4021 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4081 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4141 catgtctgta taccgtcgac ctcgaggagc tttttgcaaa agcctaggcc tccaaaaaag
     4201 cctcttcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     4261 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
     4321 atgggcggag ttaggggcgg gactatggtt gctgactaat tgctgcatta atgaatcggc
     4381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6421 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6481 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6541 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6601 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     6661 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     6721 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     6781 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     6841 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     6901 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     6961 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7021 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7081 ccgcgctcaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     7141 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7201 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7261 ggccagtgaa ttgtaatacg actcactata ggacgcgtcc tagttattaa tagtaatcaa
     7321 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     7381 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7441 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7501 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7561 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7621 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     7681 cgttctgctt cactctcccc atctcccccc ctccccaccc ccaattttgt atttatttat
     7741 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg
     7801 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     7861 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     7921 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     7981 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     8041 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     8101 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     8161 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     8221 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     8281 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     8341 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     8401 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     8461 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     8521 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     8581 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     8641 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     8701 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     8761 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     8821 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     8881 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     8941 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     9001 tggcaaagaa ttcggatcca ccggccggtc gccacc
//
