LOCUS       pHRE_R18-2              5116 bp    DNA     circular UNA 26-APR-2023
DEFINITION  .
ACCESSION   urn.local...7k9-d7xyp4s
VERSION     urn.local...7k9-d7xyp4s
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        19..54
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     source          <20..>328
                     /organism="Sequence"
                     /mol_type="other DNA"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
     enhancer        60..95
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        101..136
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        142..177
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        183..218
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     promoter        229..288
                     /note="mCMV"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mCMV promoter"
     regulatory      323..332
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiationof translation (Kozak, 1987)"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="regulatory"
     gene            329..745
                     /note="UnaG"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="UnaG"
     gene            752..>880
                     /note
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PEST"
     misc_feature    914..943
                     /note="myc protein tag derived from c-myc oncogene"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="myc"
     polyA_signal    3016..3137
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 poly(A) signal"
     terminator      3016..3137
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(3220..3239)
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VR"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(3339..3927)
     misc_RNA        3781..3888
                     /note="RNAI transcript"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="RNAI transcript"
     CDS             complement(4098..4958)
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Ampicillin"
     gene            complement(4098..4958)
                     /gene="bla"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bla gene"
     sig_peptide     complement(4890..4958)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bla signal peptide"
     misc_feature    complement(4959..5057)
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bla promoter"
                     /note="Geneious type: promoter prokaryotic"
     promoter        complement(4959..5063)
                     /gene="bla"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gtaccgagct tttctcgagc cacagtgcat acgtgggctc caacaggtcc tcttgtcgag
       61 ccacagtgca tacgtgggct ccaacaggtc ctcttgtcga gccacagtgc atacgtgggc
      121 tccaacaggt cctcttgtcg agccacagtg catacgtggg ctccaacagg tcctcttgtc
      181 gagccacagt gcatacgtgg gctccaacag gtcctcttgt cgagatctgg taggcgtgta
      241 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcac tagaagcttg
      301 gcattccggt actgttggta aagccaccat ggtcgagaaa tttgttggca cctggaagat
      361 cgcagacagc cataattttg gtgaatacct gaaagctatc ggagccccaa aggaattaag
      421 cgatggtggg gatgccacga cgccgacatt gtacatctcc cagaaggacg gagacaaaat
      481 gacagtgaaa atagagaatg gacctcctac gttccttgac actcaagtaa agttcaaatt
      541 aggggaggag ttcgacgaat ttccttctga tcgaagaaaa ggcgtaaaat ctgtcgtgaa
      601 cttggtggga gagaagctgg tgtacgtaca aaagtgggac ggcaaggaga cgacgtatgt
      661 ccgagagata aaggacggta aactggtcgt gacacttacg atgggagacg tcgtggctgt
      721 gcgcagctac cggagggcga cggaaaccgg taagaagctt agccacggct tcccgccgga
      781 ggtggaggag caggatgatg gcacgctgcc catgtcttgt gcccaggaga gcgggatgga
      841 ccgtcaccct gcagcctgtg cttctgctag gatcaatgtg gtcgacccgc ggggatccac
      901 gcgtgcggcc gcagaacaaa aactcatctc agaagaggat ctgaatgggg ccgcatagtc
      961 tagaagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     1021 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     1081 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     1141 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatggcccgg
     1201 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     1261 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1321 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1381 ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt
     1441 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     1501 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1561 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     1621 ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1681 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     1801 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     1861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1921 taggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     1981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     2041 gtccacaatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2101 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2161 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2221 tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2281 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     2341 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     2401 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     2461 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     2521 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgtat
     2581 gcccgacggc gaggatctcg tcgtgactca tggcgatgcc tgcttgccga atatcatggt
     2641 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     2701 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     2761 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     2821 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     2881 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     2941 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     3001 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3121 ctcatcaatg tatcttatca tgtctgtata ccggatcttt ccgcttcctc gctcactgac
     3181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcagatcg
     5101 acggatcggg agatcg
//
