LOCUS       pHRE_UnaG_ODDD_R18-3        5806 bp    DNA     circular UNA 26-APR-2023
DEFINITION  .
ACCESSION   urn.local...7kb-d7xyp4s
VERSION     urn.local...7kb-d7xyp4s
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        19..54
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        60..95
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        101..136
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        142..177
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     enhancer        183..218
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HRE_enhancer"
     promoter        229..288
                     /note="mCMV"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="mCMV promoter"
     regulatory      323..332
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiationof translation (Kozak, 1987)"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="regulatory"
     gene            329..745
                     /note="UnaG"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="UnaG"
     gene            761..1574
                     /note="ODDD"
                     /note="Derived using Geneious Prime 2021.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ODDD gene"
     misc_feature    1498..1536
                     /standard_name="GGAAGGCATTTCCGCGAGCCCTGAAAGCGCAAGTCCTC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1604..1633
                     /note="myc protein tag derived from c-myc oncogene"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="myc"
     polyA_signal    3706..3827
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 poly(A) signal"
     terminator      3706..3827
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     misc_feature    complement(3910..3929)
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VR"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(4029..4617)
     misc_RNA        4471..4578
                     /note="RNAI transcript"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="RNAI transcript"
     CDS             complement(4788..5648)
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Ampicillin"
     gene            complement(4788..5648)
                     /gene="bla"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bla gene"
     sig_peptide     complement(5580..5648)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bla signal peptide"
     misc_feature    complement(5649..5747)
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bla promoter"
                     /note="Geneious type: promoter prokaryotic"
     promoter        complement(5649..5753)
                     /gene="bla"
                     /note="Derived using Geneious Prime 2021.0.0 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gtaccgagct tttctcgagc cacagtgcat acgtgggctc caacaggtcc tcttgtcgag
       61 ccacagtgca tacgtgggct ccaacaggtc ctcttgtcga gccacagtgc atacgtgggc
      121 tccaacaggt cctcttgtcg agccacagtg catacgtggg ctccaacagg tcctcttgtc
      181 gagccacagt gcatacgtgg gctccaacag gtcctcttgt cgagatctgg taggcgtgta
      241 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcac tagaagcttg
      301 gcattccggt actgttggta aagccaccat ggtcgagaaa tttgttggca cctggaagat
      361 cgcagacagc cataattttg gtgaatacct gaaagctatc ggagccccaa aggaattaag
      421 cgatggtggg gatgccacga cgccgacatt gtacatctcc cagaaggacg gagacaaaat
      481 gacagtgaaa atagagaatg gacctcctac gttccttgac actcaagtaa agttcaaatt
      541 aggggaggag ttcgacgaat ttccttctga tcgaagaaaa ggcgtaaaat ctgtcgtgaa
      601 cttggtggga gagaagctgg tgtacgtaca aaagtgggac ggcaaggaga cgacgtatgt
      661 ccgagagata aaggacggta aactggtcgt gacacttacg atgggagacg tcgtggctgt
      721 gcgcagctac cggagggcga cggaaaccgg tgtcgacgga gtgaattacg ttgtgagtgg
      781 tattattcag cacgacttga ttttctccct tcaacaaaca gaatgtgtcc ttaaaccggt
      841 tgaatcttca gatatgaaaa tgactcagct attcaccaaa gttgaatcag aagatacaag
      901 tagcctcttt gacaaactta agaaggaacc tgatgcttta actttgctgg ccccagccgc
      961 tggagacaca atcatatctt tagattttgg cagcaacgac acagaaactg atgaccagca
     1021 acttgaggaa gtaccattat ataatgatgt aatgctcccc tcacccaacg aaaaattaca
     1081 gaatataaat ttggcaatgt ctccattacc caccgctgaa acgccaaagc cacttcgaag
     1141 tagtgctgac cctgcactca atcaagaagt tgcattaaaa ttagaaccaa atccagagtc
     1201 actggaactt tcttttacca tgccccagat tcaggatcag acacctagtc cttccgatgg
     1261 aagcactaga caaagttcac ctgagcctaa tagtcccagt gaatattgtt tttatgtgga
     1321 tagtgatatg gtcaatgaat tcaagttgga attggtagaa aaactttttg ctgaagacac
     1381 agaagcaaag aacccatttt ctactcagga cacagattta gacttggaga tgttagctcc
     1441 ctatatccca atggatgatg acttccagtt acgttccttc gatcagttgt caccattaga
     1501 aagcagttcc gcaagccctg aaagcgcaag tcctcaaagc acagttacag tattccagca
     1561 gactcaaata caaggaccgc ggggatccac gcgtgcggcc gcagaacaaa aactcatctc
     1621 agaagaggat ctgaatgggg ccgcatagtc tagaagctcg ctgatcagcc tcgactgtgc
     1681 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     1741 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     1801 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     1861 acaatagcag gcatgctggg gatggcccgg gctctatggc ttctgaggcg gaaagaacca
     1921 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2101 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2161 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2281 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa
     2341 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     2401 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt
     2461 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2521 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2581 ctccgcccag ttccgcccat tctccgcccc taggctgact aatttttttt atttatgcag
     2641 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2701 gcctaggctt ttgcaaaaag ctcccgggag gtccacaatg attgaacaag atggattgca
     2761 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     2821 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     2881 tgtcaagacc gacctgtccg gtgccctgaa tgaactccag gacgaggcag cgcggctatc
     2941 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3001 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3061 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3121 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3181 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3241 cgaactgttc gccaggctca aggcgcgtat gcccgacggc gaggatctcg tcgtgactca
     3301 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3361 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3421 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3481 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     3541 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     3601 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     3661 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     3721 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3781 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     3841 ccggatcttt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4201 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     4261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4441 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4801 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4861 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4921 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4981 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5041 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5101 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5161 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5221 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5281 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5341 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5401 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5461 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5521 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5581 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5641 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5701 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5761 ccccgaaaag tgccacctga cgtcagatcg acggatcggg agatcg
//
