LOCUS       Exported                4345 bp DNA     circular SYN 26-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /label=AmpR promoter
                     /note="gene : bla"
     CDS             126..986
                     /codon_start=1
                     /label=AmpR
                     /note="gene : bla-beta-lactamaseconfers resistance to 
                     ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1910..1931
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1946..1976
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    1958..1976
                     /label=PhD_seq_F
     protein_bind    1984..2000
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2025..2083
                     /codon_start=1
                     /label=plB
                     /translation="MKYLLPTAAAGLLLLAAQP"
     CDS             2466..2492
                     /codon_start=1
                     /label=BAP-His
                     /translation="GHHHHHH*"
     CDS             2525..2546
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="THLRRGS"
     misc_feature    complement(2605..2624)
                     /label=PhD_seq_R
     rep_origin      complement(3911..4339)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of ( ) strand synthesis"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggccatggcc caggtgcagc
     2101 tggtcgagtc tgggggagga ttggtgcagg ctggaggctc tctgagactc tcctgtgcag
     2161 cctctggagg caccttcagt accgctgcca tgggctggtt ccgccaggct ccaggcgagg
     2221 agcgtgagtg tgtagcatct ataggctgga gaggtgttag aacatggtat gcagactccg
     2281 tgaagggccg atttaccatc tccagagata atccccagaa tacggtgtat ttgcagatga
     2341 acaacctgaa atctggcgac acggccgttt attactgcgc agcctcagtc ggcaactacg
     2401 ggttgccgtg ggcccacttc gaatatgact tctggggcca ggggatccag gtcaccgtgt
     2461 cgtcaggcca ccatcaccat caccattaat aaccaccatc atggcgcaga acaaaccagc
     2521 cggcactcat ctcagaagag gatctgtctt aggccgaaac tgttgaaagt tgtttagcaa
     2581 aacctcatac agaaaattca tttactaacg tctggaaaga cgacaaaact ttagatcgtt
     2641 acgctaacta tgagggctgt ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg
     2701 aaactcagtg ttacggtaca tgggttccta ttgggcttgc tatccctgaa aatgagggtg
     2761 gtggctctga gggtggcggt tctgagggtg gcggttctga gggtggcggt actaaacctc
     2821 ctgagtacgg tgatacacct attccgggct atacttatat caaccctctc gacggcactt
     2881 atccgcctgg tactgagcaa aaccccgcta atcctaatcc ttctcttgag gagtctcagc
     2941 ctcttaatac tttcatgttt cagaataata ggttccgaaa taggcagggt gcattaactg
     3001 tttatacggg cactgttact caaggcactg accccgttaa aacttattac cagtacactc
     3061 ctgtatcatc aaaagccatg tatgacgctt actggaacgg taaattcaga gactgcgctt
     3121 tccattctgg ctttaatgag gatccattcg tttgtgaata tcaaggccaa tcgtctgacc
     3181 tgcctcaacc tcctgtcaat gctggcggcg gctctggtgg tggttctggt ggcggctctg
     3241 agggtggcgg ctctgagggt ggcggttctg agggtggcgg ctctgagggt ggcggttccg
     3301 gtggcggctc cggttccggt gattttgatt atgaaaaaat ggcaaacgct aataaggggg
     3361 ctatgaccga aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt
     3421 ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg
     3481 ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg
     3541 gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct tctttgcctc
     3601 agtcggttga atgtcgccct tatgtctttg gcgctggtaa accatatgaa ttttctattg
     3661 attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct
     3721 ttatgtatgt attttcgacg tttgctaaca tactgcgtaa taaggagtct taagctagct
     3781 aacagtccta tgaatcaact acttagatgg tattagtgac ctgtaacaga gcattagcgc
     3841 aaggtgattt ttgtcttctt gcgctaattt tttgtcatca aacctgtcgc actccttaat
     3901 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     3961 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4021 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4081 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4141 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4201 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4261 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4321 gcgccgctac agggcgcgtc aggtg
//
