LOCUS       Exported                4348 bp DNA     circular SYN 26-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /label=AmpR promoter
                     /note="gene : bla"
     CDS             126..986
                     /codon_start=1
                     /label=AmpR
                     /note="gene : bla-beta-lactamaseconfers resistance to 
                     ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1910..1931
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1946..1976
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    1958..1976
                     /label=PhD_seq_F
     protein_bind    1984..2000
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2025..2083
                     /codon_start=1
                     /label=plB
                     /translation="MKYLLPTAAAGLLLLAAQP"
     CDS             2469..2495
                     /codon_start=1
                     /label=BAP-His
                     /translation="GHHHHHH*"
     CDS             2528..2549
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="THLRRGS"
     misc_feature    complement(2608..2627)
                     /label=PhD_seq_R
     rep_origin      complement(3914..4342)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of ( ) strand synthesis"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggccatggcc caggtgcagc
     2101 tggtcgagtc tgggggaggc ttggtgcagc ctggggggtc tctgacactc tcctgtgtag
     2161 cgtcggaatc atctttggcg ccttatcgcg tagcctggtt ccgtcaggcc ccagggaagg
     2221 agcgcgaggg ggtctcatgt attagtcgtg atgcacatcc tactagtaca tactatacag
     2281 cctccgtgaa gggccgattc accatgtcca gagacaatgc caagaacacg gtgtatctgc
     2341 aaatgaacag cctgaaacca tcggacacgg ccgtttatta ctgtgcgaca gatttgggag
     2401 gatactgttc ggattctaat tatccccgcg cctggtgggg ccaggggacc caggtcaccg
     2461 tctcctcagg ccaccatcac catcaccatt aataaccacc atcatggcgc agaacaaacc
     2521 agccggcact catctcagaa gaggatctgt cttaggccga aactgttgaa agttgtttag
     2581 caaaacctca tacagaaaat tcatttacta acgtctggaa agacgacaaa actttagatc
     2641 gttacgctaa ctatgagggc tgtctgtgga atgctacagg cgttgtggtt tgtactggtg
     2701 acgaaactca gtgttacggt acatgggttc ctattgggct tgctatccct gaaaatgagg
     2761 gtggtggctc tgagggtggc ggttctgagg gtggcggttc tgagggtggc ggtactaaac
     2821 ctcctgagta cggtgataca cctattccgg gctatactta tatcaaccct ctcgacggca
     2881 cttatccgcc tggtactgag caaaaccccg ctaatcctaa tccttctctt gaggagtctc
     2941 agcctcttaa tactttcatg tttcagaata ataggttccg aaataggcag ggtgcattaa
     3001 ctgtttatac gggcactgtt actcaaggca ctgaccccgt taaaacttat taccagtaca
     3061 ctcctgtatc atcaaaagcc atgtatgacg cttactggaa cggtaaattc agagactgcg
     3121 ctttccattc tggctttaat gaggatccat tcgtttgtga atatcaaggc caatcgtctg
     3181 acctgcctca acctcctgtc aatgctggcg gcggctctgg tggtggttct ggtggcggct
     3241 ctgagggtgg cggctctgag ggtggcggtt ctgagggtgg cggctctgag ggtggcggtt
     3301 ccggtggcgg ctccggttcc ggtgattttg attatgaaaa aatggcaaac gctaataagg
     3361 gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg
     3421 attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac gtttccggcc
     3481 ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag
     3541 tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta ccttctttgc
     3601 ctcagtcggt tgaatgtcgc ccttatgtct ttggcgctgg taaaccatat gaattttcta
     3661 ttgattgtga caaaataaac ttattccgtg gtgtctttgc gtttctttta tatgttgcca
     3721 cctttatgta tgtattttcg acgtttgcta acatactgcg taataaggag tcttaagcta
     3781 gctaacagtc ctatgaatca actacttaga tggtattagt gacctgtaac agagcattag
     3841 cgcaaggtga tttttgtctt cttgcgctaa ttttttgtca tcaaacctgt cgcactcctt
     3901 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3961 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4021 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4081 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4141 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4201 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4261 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4321 aatgcgccgc tacagggcgc gtcaggtg
//
