LOCUS       Exported                4360 bp DNA     circular SYN 06-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /label=AmpR promoter
                     /note="gene : bla"
     CDS             126..986
                     /codon_start=1
                     /label=AmpR
                     /note="gene : bla-beta-lactamaseconfers resistance to 
                     ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1910..1931
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1946..1976
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    1958..1976
                     /label=PhD_seq_F
     protein_bind    1984..2000
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2025..2083
                     /codon_start=1
                     /label=plB
                     /translation="MKYLLPTAAAGLLLLAAQP"
     CDS             2478..2507
                     /codon_start=1
                     /label=BAP-His
                     /translation="SGHHHHHH*"
     CDS             2540..2561
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="THLRRGS"
     misc_feature    complement(2620..2639)
                     /label=PhD_seq_R
     rep_origin      complement(3926..4354)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of ( ) strand synthesis"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggccatggcc caggtgcagc
     2101 tggtcgagca ggtgcagctg gtcgagtctg ggggaggctt ggtgcagcct ggggggtctc
     2161 tgagactctc ctgtgcagtc tttggatcga ctttggataa ttatagcgta ggctggttcc
     2221 gccagctccc agggaaggag cgcgagggcc tggcatgttt tagtcgtagg tatggcgccc
     2281 catactacgc agactctgcg aaggaccgat tcaccatttc cagagacaat gccaagaaca
     2341 cggtcgattt gcaactgaac aacctgaaac ctgaggacac ggccgtctat tactgtgcga
     2401 caagatccgg accgtactgt acaactagcg tgtctgactt tgatgactgg ggccggggga
     2461 cccaggtcac cgtctcctca ggccaccatc accatcacca ttaataacca ccatcatggc
     2521 gcagaacaaa ccagccggca ctcatctcag aagaggatct gtcttaggcc gaaactgttg
     2581 aaagttgttt agcaaaacct catacagaaa attcatttac taacgtctgg aaagacgaca
     2641 aaactttaga tcgttacgct aactatgagg gctgtctgtg gaatgctaca ggcgttgtgg
     2701 tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg cttgctatcc
     2761 ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt tctgagggtg
     2821 gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact tatatcaacc
     2881 ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct aatccttctc
     2941 ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc cgaaataggc
     3001 agggtgcatt aactgtttat acgggcactg ttactcaagg cactgacccc gttaaaactt
     3061 attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg aacggtaaat
     3121 tcagagactg cgctttccat tctggcttta atgaggatcc attcgtttgt gaatatcaag
     3181 gccaatcgtc tgacctgcct caacctcctg tcaatgctgg cggcggctct ggtggtggtt
     3241 ctggtggcgg ctctgagggt ggcggctctg agggtggcgg ttctgagggt ggcggctctg
     3301 agggtggcgg ttccggtggc ggctccggtt ccggtgattt tgattatgaa aaaatggcaa
     3361 acgctaataa gggggctatg accgaaaatg ccgatgaaaa cgcgctacag tctgacgcta
     3421 aaggcaaact tgattctgtc gctactgatt acggtgctgc tatcgatggt ttcattggtg
     3481 acgtttccgg ccttgctaat ggtaatggtg ctactggtga ttttgctggc tctaattccc
     3541 aaatggctca agtcggtgac ggtgataatt cacctttaat gaataatttc cgtcaatatt
     3601 taccttcttt gcctcagtcg gttgaatgtc gcccttatgt ctttggcgct ggtaaaccat
     3661 atgaattttc tattgattgt gacaaaataa acttattccg tggtgtcttt gcgtttcttt
     3721 tatatgttgc cacctttatg tatgtatttt cgacgtttgc taacatactg cgtaataagg
     3781 agtcttaagc tagctaacag tcctatgaat caactactta gatggtatta gtgacctgta
     3841 acagagcatt agcgcaaggt gatttttgtc ttcttgcgct aattttttgt catcaaacct
     3901 gtcgcactcc ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     3961 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     4021 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     4081 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     4141 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     4201 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     4261 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     4321 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
//
