LOCUS       pmEGFP-tHe-Tub1A2       6060 bp ds-DNA     circular     25-MAY-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     SEQUENCED       558..1654
                     /label="SEQ-CMV-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          572..618
                     /label="mEGFP_F_Tub1c1_Hifi"
                     /note="sequence: ttagtgaaccgtcagatccgctagccaccatggtgagcaagggcgag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             601..1317
                     /label="mEGFP"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             601..1317
                     /label="Translation 601-1317"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKEFVTAAGITLGMDELYK"
     primer          complement(1298..1348)
                     /label="mEGFP_Tub1C1_R-Hifi"
                     /note="sequence: TGCATTCGCGcactcgagatctgagtccggacttgtacagctcgtccatgc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1318..1338
                     /label="Translation 1318-1338"
                     /translation="SGLRSRV"
     primer          1329..1356
                     /label="Tub1C1n_F-HIFi"
                     /note="sequence: atctcgagtgCGCGAATGCATCTCTATC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1339..2685
                     /label="tHe-Tub1A2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1339..2685
                     /label="Translation 1339-2685"
                     /translation="RECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGAADDSFNTFFSETGSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIVDLVLDRVRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVNNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPIISAEKAYHEQLTVSEITNSCFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGVDSVDAGEEGEEY*"
     misc_feature    complement(2188..2858)
                     /label="SV40-pA-R-seq"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2668..2705)
                     /label="Tub1C1n_R-HIFI"
                     /note="sequence: ccgtcgactgcagaattcgaTCAATATTCCTCGCCTTC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2848..2969
                     /label="SV40 poly A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2976..3431)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3772..3907
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3956..4750
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5358..5946
                     /label="ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      661 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      721 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      781 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      841 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      901 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      961 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1021 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1081 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1141 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1201 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1261 cttaaggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
     1321 ggactcagat ctcgagtgCG CGAATGCATC TCTATCCACG TCGGACAGGC CGGAGTGCAG
     1381 ATTGGCAACG CCTGCTGGGA GCTTTACTGT CTGGAGCATG GCATCCAGCC GGACGGGCAG
     1441 ATGCCGTCGG ACAAGACGGT TGGCGCCGCT GACGACTCCT TCAACACCTT CTTCAGCGAG
     1501 ACCGGATCCG GCAAGCACGT TCCGCGGGCC GTCTTCGTCG ATCTCGAGCC GACCGTTGTT
     1561 GATGAAGTGC GAACCGGAAC ATACCGCCAG CTTTTCCATC CAGAGCAGCT AATCACCGGA
     1621 AAGGAGGATG CCGCCAACAA TTACGCTCGC GGGCACTACA CCGTCGGCAA GGAGATTGTC
     1681 GACCTGGTGC TGGACCGCGT GCGCAAGCTC GCCGACCAAT GTACCGGTCT GCAGGGCTTT
     1741 CTCATCTTTC ACTCGTTCGG CGGTGGGACC GGATCCGGAT TCACCTCCCT GCTGATGGAA
     1801 CGCTTGTCGG TCGACTACGG GAAGAAGTCC AAACTGGAAT TCTCCATCTA CCCCGCCCCT
     1861 CAGGTGTCGA CTGCCGTGGT GGAGCCGTAC AACTCGATCC TGACGACCCA TACCACGCTG
     1921 GAGCACTCTG ACTGTGCCTT CATGGTGAAC AACGAAGCCA TCTACGACAT TTGCCGTCGG
     1981 AACCTTGACA TTGAGCGTCC GACCTACACC AACCTCAACC GCCTGATCGG GCAGATTGTC
     2041 TCCTCCATCA CCGCGTCGCT GCGCTTCGAC GGCGCTCTCA ACGTCGATCT CACCGAATTC
     2101 CAGACCAACT TGGTTCCTTA CCCCCGTATC CATTTCCCGC TGGTGACCTA CGCCCCGATC
     2161 ATTTCCGCTG AAAAGGCCTA TCACGAACAG TTGACGGTCT CGGAGATCAC CAACTCGTGC
     2221 TTCGAGCCGG CTAACCAGAT GGTCAAGTGC GACCCGCGCC ACGGAAAGTA CATGGCCTGC
     2281 TGCATGCTCT ACCGCGGTGA CGTTGTGCCC AAGGACGTGA ACGCCGCCAT CGCGGCCATT
     2341 AAGACCAAGC GCAGCATCCA GTTTGTCGAC TGGTGCCCGA CTGGATTTAA GGTGGGAATC
     2401 AACTATCAGC CCCCGACGGT GGTGCCGGGC GGGGATTTGG CCAAGGTGCA GCGTGCCGTC
     2461 TGCATGCTGT CCAACACCAC GGCCATCGCC GAAGCCTGGG CGCGTCTGGA CCACAAGTTC
     2521 GACCTGATGT ACGCCAAGCG CGCCTTTGTC CACTGGTACG TCGGTGAGGG CATGGAGGAG
     2581 GGCGAGTTCA GCGAGGCCCG TGAAGATTTG GCCGCCCTCG AGAAGGACTA CGAGGAGGTC
     2641 GGTGTGGACT CTGTCGACGC TGGTGAGGAA GGCGAGGAAT ATTGAtcgaa ttctgcagtc
     2701 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     2761 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2821 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2941 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     3001 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3061 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3121 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3181 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3241 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3301 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3361 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3421 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3481 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3541 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     3601 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3721 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3901 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     3961 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4021 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4081 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     4141 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4201 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4261 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4321 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4381 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4441 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     4501 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4561 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4621 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4681 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4741 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4801 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4861 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4921 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     4981 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     5041 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     5101 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     5161 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     5221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//