LOCUS       Exported                6801 bp ds-DNA     circular SYN 22-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 28, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pOTTC1845 was constructed by Chris Richie at NIDA/GEVVC (CR2395_L).
            QA with ApaLI+MluI+PmlI
            
            backbone was pOTTC241, Obtained from Karl Deisseroth; matches 
            Addgene 26969
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=ITR (AAV)
     promoter        156..1448
                     /label=a-CaMKII(1.3)
                     /note="/vntifkey=21"
     CDS             1488..3509
                     /label=hChR2(H134R)-V5-ER-LBD
     protein_bind    2481..2522
                     /label=V5 epitope [GKPIPNPLLGLDST]
     misc_recomb     complement(3517..3533)
                     /label=GEVVC AscI Infusion linker
     polyA_signal    3547..4027
                     /label=hGH polyA
     LTR             complement(4064..4204)
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     rep_origin      4279..4734
                     /label=M13 origin
     rep_origin      4296..4602
                     /label=f1 origin
     promoter        5022..5120
                     /label=Bla promoter (prokaryotic)
     CDS             5121..5978
                     /label=AmpR
     rep_origin      6076..6758
                     /label=ColE1 origin
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccggatcccg aagttatcgc tagcaccatg gactatggcg
     1501 gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg
     1561 ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca
     1621 cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt
     1681 tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct
     1741 atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc
     1801 cctctatgct ctaccttgcc acaggacacc gggtgcagtg gctgcgctat gcagagtggc
     1861 tgctcacttg tcctgtcatc cttatccgcc tgagcaacct caccggcctg agcaacgact
     1921 acagcaggag aaccatggga ctccttgtct cagacatcgg gactatcgtg tggggggcta
     1981 ccagcgccat ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga ttgtgctatg
     2041 gcgcgaacac attttttcac gccgccaaag catatatcga gggttatcat actgtgccaa
     2101 agggtcggtg ccgccaggtc gtgaccggca tggcatggct gtttttcgtg agctggggta
     2161 tgttcccaat tctcttcatt ttggggcccg aaggttttgg cgtcctgagc gtctatggct
     2221 ccaccgtagg tcacacgatt attgatctga tgagtaaaaa ttgttggggg ttgttgggac
     2281 actacctgcg cgtcctgatc cacgagcaca tattgattca cggagatatc cgcaaaacca
     2341 ccaaactgaa catcggcgga acggagatcg aggtcgagac tctcgtcgaa gacgaagccg
     2401 aggccggagc cgtgccagcg gccgccaccg gtggatctat ggaagcttac tgtcatatta
     2461 aggggttcga tgccgtgaat ggaaagccaa tacccaatcc tctgctgggc ttggactcaa
     2521 ctagaaccgg cggcggttgt ccatgggcca ctggttcagg ttcatctgct ggagacatga
     2581 gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg
     2641 ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac
     2701 tctattccga gtatgatcct accagaccct tcagtgaagc ctccatgatg ggcttactga
     2761 ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct
     2821 ttgtggattt gaccctccat gatcaggtcc accttctgga atgtgcctgg ctagagatcc
     2881 tgatgattgg cctcgtctgg cgctccatgg agcacccagg gaagctactg tttgctccta
     2941 acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca
     3001 tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt
     3061 gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga
     3121 agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca gacactttga
     3181 tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc
     3241 tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca
     3301 gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggagatg ctggacgccc
     3361 accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc
     3421 acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg
     3481 aggcagaggg tttccctgcc acagtctagg caggtgggcg cgccataact tcgctcgaga
     3541 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     3601 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     3661 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     3721 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3781 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3841 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3901 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3961 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     4021 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     4081 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4141 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4201 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4261 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4321 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4381 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4441 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4501 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4561 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4621 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4681 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4741 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4801 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4861 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5341 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5641 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctggccttt tgctcacatg t
//
