LOCUS       Exported               11171 bp ds-DNA     circular SYN 04-MAY-2023
DEFINITION  2nd generation lentiviral packaging plasmid. Can be used with 2nd or
            3rd generation lentiviral vectors and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    psPAX2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11171)
  TITLE     Trono Lab Packaging and Envelope Plasmids
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        31..32
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        84..324
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    333..444
                     /label=SpyM2-sgRNA (BsmBI)
     misc_feature    445..451
                     /label=U6 terminator
     enhancer        491..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1151..1834
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          1835..2167
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             2273..3775
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2669..3361
                     /codon_start=1
                     /label=CAPSID
                     /translation="PIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSE
                     GATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDI
                     AGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRD
                     YVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPGATLEEMMTACQGVG
                     GPGHKARVL"
     CDS             3568..6579
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6264..6381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7030..8178
                     /codon_start=1
                     /label=orf
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSLAL
                     IWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVSL
                     LNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL"
     misc_feature    7152..7385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_site      8284..8339
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(8700..8716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8724..8740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8748..8778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8793..8814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8873..9068
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8919..9054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9074..9208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9447..10035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10206..11066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11067..11171)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gaccgggttt attacaggga cagcagagat
       61 ccagtttggt taattaaggt accgagggcc tatttcccat gattccttca tatttgcata
      121 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      181 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      241 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      301 ctttatatat cttgtggaaa ggacgaaaca ccggagacgg aggacgacga acgtctctgt
      361 ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt
      421 gaaaaagtgg caccgagtcg gtgctttttt tgaattcgct agctaggtct tgaaaggagt
      481 gggaattgtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      541 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      841 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      901 ctccccatct ccccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
      961 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     1021 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     1081 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     1141 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     1201 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     1261 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     1321 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     1381 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     1441 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     1501 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     1561 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1621 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1681 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1741 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     1801 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1861 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1921 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1981 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     2041 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     2101 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     2161 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     2221 cgggccgcgt tgacgcgcac ggcaagaggc gaggggcggc gactggtgag agatgggtgc
     2281 gagagcgtca gtattaagcg ggggagaatt agatcgatgg gaaaaaattc ggttaaggcc
     2341 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     2401 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     2461 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     2521 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     2581 gatagaggaa gagcaaaaca aaagtaagaa aaaagcacag caagcagcag ctgacacagg
     2641 acacagcaat caggtcagcc aaaattaccc tatagtgcag aacatccagg ggcaaatggt
     2701 acatcaggcc atatcaccta gaactttaaa tgcatgggta aaagtagtag aagagaaggc
     2761 tttcagccca gaagtgatac ccatgttttc agcattatca gaaggagcca ccccacaaga
     2821 tttaaacacc atgctaaaca cagtgggggg acatcaagca gccatgcaaa tgttaaaaga
     2881 gaccatcaat gaggaagctg cagaatggga tagagtgcat ccagtgcatg cagggcctat
     2941 tgcaccaggc cagatgagag aaccaagggg aagtgacata gcaggaacta ctagtaccct
     3001 tcaggaacaa ataggatgga tgacacataa tccacctatc ccagtaggag aaatctataa
     3061 aagatggata atcctgggat taaataaaat agtaagaatg tatagcccta ccagcattct
     3121 ggacataaga caaggaccaa aggaaccctt tagagactat gtagaccgat tctataaaac
     3181 tctaagagcc gagcaagctt cacaagaggt aaaaaattgg atgacagaaa ccttgttggt
     3241 ccaaaatgcg aacccagatt gtaagactat tttaaaagca ttgggaccag gagcgacact
     3301 agaagaaatg atgacagcat gtcagggagt ggggggaccc ggccataaag caagagtttt
     3361 ggctgaagca atgagccaag taacaaatcc agctaccata atgatacaga aaggcaattt
     3421 taggaaccaa agaaagactg ttaagtgttt caattgtggc aaagaagggc acatagccaa
     3481 aaattgcagg gcccctagga aaaagggctg ttggaaatgt ggaaaggaag gacaccaaat
     3541 gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt cccacaaggg
     3601 aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag aagagagctt
     3661 caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag acaaggaact
     3721 gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac aataaagata
     3781 ggggggcaat taaaggaagc tctattagat acaggagcag atgatacagt attagaagaa
     3841 atgaatttgc caggaagatg gaaaccaaaa atgatagggg gaattggagg ttttatcaaa
     3901 gtaagacagt atgatcagat actcatagaa atctgcggac ataaagctat aggtacagta
     3961 ttagtaggac ctacacctgt caacataatt ggaagaaatc tgttgactca gattggctgc
     4021 actttaaatt ttcccattag tcctattgag actgtaccag taaaattaaa gccaggaatg
     4081 gatggcccaa aagttaaaca atggccattg acagaagaaa aaataaaagc attagtagaa
     4141 atttgtacag aaatggaaaa ggaaggaaaa atttcaaaaa ttgggcctga aaatccatac
     4201 aatactccag tatttgccat aaagaaaaaa gacagtacta aatggagaaa attagtagat
     4261 ttcagagaac ttaataagag aactcaagat ttctgggaag ttcaattagg aataccacat
     4321 cctgcagggt taaaacagaa aaaatcagta acagtactgg atgtgggcga tgcatatttt
     4381 tcagttccct tagataaaga cttcaggaag tatactgcat ttaccatacc tagtataaac
     4441 aatgagacac cagggattag atatcagtac aatgtgcttc cacagggatg gaaaggatca
     4501 ccagcaatat tccagtgtag catgacaaaa atcttagagc cttttagaaa acaaaatcca
     4561 gacatagtca tctatcaata catggatgat ttgtatgtag gatctgactt agaaataggg
     4621 cagcatagaa caaaaataga ggaactgaga caacatctgt tgaggtgggg atttaccaca
     4681 ccagacaaaa aacatcagaa agaacctcca ttcctttgga tgggttatga actccatcct
     4741 gataaatgga cagtacagcc tatagtgctg ccagaaaagg acagctggac tgtcaatgac
     4801 atacagaaat tagtgggaaa attgaattgg gcaagtcaga tttatgcagg gattaaagta
     4861 aggcaattat gtaaacttct taggggaacc aaagcactaa cagaagtagt accactaaca
     4921 gaagaagcag agctagaact ggcagaaaac agggagattc taaaagaacc ggtacatgga
     4981 gtgtattatg acccatcaaa agacttaata gcagaaatac agaagcaggg gcaaggccaa
     5041 tggacatatc aaatttatca agagccattt aaaaatctga aaacaggaaa gtatgcaaga
     5101 atgaagggtg cccacactaa tgatgtgaaa caattaacag aggcagtaca aaaaatagcc
     5161 acagaaagca tagtaatatg gggaaagact cctaaattta aattacccat acaaaaggaa
     5221 acatgggaag catggtggac agagtattgg caagccacct ggattcctga gtgggagttt
     5281 gtcaataccc ctcccttagt gaagttatgg taccagttag agaaagaacc cataatagga
     5341 gcagaaactt tctatgtaga tggggcagcc aatagggaaa ctaaattagg aaaagcagga
     5401 tatgtaactg acagaggaag acaaaaagtt gtccccctaa cggacacaac aaatcagaag
     5461 actgagttac aagcaattca tctagctttg caggattcgg gattagaagt aaacatagtg
     5521 acagactcac aatatgcatt gggaatcatt caagcacaac cagataagag tgaatcagag
     5581 ttagtcagtc aaataataga gcagttaata aaaaaggaaa aagtctacct ggcatgggta
     5641 ccagcacaca aaggaattgg aggaaatgaa caagtagata aattggtcag tgctggaatc
     5701 aggaaagtac tatttttaga tggaatagat aaggcccaag aagaacatga gaaatatcac
     5761 agtaattgga gagcaatggc tagtgatttt aacctaccac ctgtagtagc aaaagaaata
     5821 gtagccagct gtgataaatg tcagctaaaa ggggaagcca tgcatggaca agtagactgt
     5881 agcccaggaa tatggcagct agattgtaca catttagaag gaaaagttat cttggtagca
     5941 gttcatgtag ccagtggata tatagaagca gaagtaattc cagcagagac agggcaagaa
     6001 acagcatact tcctcttaaa attagcagga agatggccag taaaaacagt acatacagac
     6061 aatggcagca atttcaccag tactacagtt aaggccgcct gttggtgggc ggggatcaag
     6121 caggaatttg gcattcccta caatccccaa agtcaaggag taatagaatc tatgaataaa
     6181 gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa gacagcagta
     6241 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     6301 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     6361 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggaaa
     6421 ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga taatagtgac
     6481 ataaaagtag tgccaagaag aaaagcaaag atcatcaggg attatggaaa acagatggca
     6541 ggtgatgatt gtgtggcaag tagacaggat gaggattaac acatggaatt ctgcaacaac
     6601 tgctgtttat ccatttcaga attgggttcg acatagcaga ataggcgtta ctcgacagag
     6661 gagagcaaga aatggagcca gtagatccta gactagagcc ctggaagcat ccaggaagtc
     6721 agcctaaaac tgcttgtacc aattgctatt gtaaaaagtg ttgctttcat tgccaagttt
     6781 gtttcatgac aaaagcctta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa
     6841 gagctcatca gaacagtcag actcatcaag cttctctatc aaagcagtaa gtagtacatg
     6901 taatgcaacc tataatagta gcaatagtag cattagtagt agcaataata atagcaatag
     6961 ttgtgtggtc catagtaatc atagaatata ggaaaatggc cgctgatctt cagacctgga
     7021 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7081 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7141 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7201 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7261 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7321 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7381 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7441 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7501 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7561 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7621 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7681 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7741 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     7801 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     7861 tccttggcac ttatctggga cgatctgcgg agcctgtgcc tcttcagcta ccaccgcttg
     7921 agagacttac tcttgattgt aacgaggatt gtggaacttc tgggacgcag ggggtgggaa
     7981 gccctcaaat attggtggaa tctcctacaa tattggagtc aggagctaaa gaatagtgct
     8041 gttagcttgc tcaatgccac agccatagca gtagctgagg ggacagatag ggttatagaa
     8101 gtagtacaag gagcttgtag agctattcgc cacataccta gaagaataag acagggcttg
     8161 gaaaggattt tgctataagc tcgaaacaac cggtacctct agaactatag ctagcagatc
     8221 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     8281 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     8341 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     8401 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     8461 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     8521 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     8581 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     8641 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     8701 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8761 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8821 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     8881 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8941 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9001 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9061 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9121 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9181 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     9241 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10801 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11161 ggggttccgc g
//
