LOCUS       Exported               11166 bp ds-DNA     circular SYN 04-MAY-2023
DEFINITION  2nd generation lentiviral packaging plasmid. Can be used with 2nd or
            3rd generation lentiviral vectors and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    psPAX2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11166)
  TITLE     Trono Lab Packaging and Envelope Plasmids
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        31..32
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        84..324
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    333..439
                     /label=SpyM2-sgRNA (BsmBI)
     misc_feature    440..446
                     /label=U6 terminator
     enhancer        486..865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1146..2162
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          1146..1829
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          1830..2162
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             2268..3770
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             3563..6574
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6259..6376
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7147..7380
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7565..7609
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_site      8279..8334
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     polyA_signal    8279..8334
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8695..8711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8719..8735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8743..8773)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8788..8809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8868..9063
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8914..9049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9069..9203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9442..10030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10201..11061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11062..11166)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gaccgggttt attacaggga cagcagagat
       61 ccagtttggt taattaaggt accgagggcc tatttcccat gattccttca tatttgcata
      121 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      181 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      241 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      301 ctttatatat cttgtggaaa ggacgaaaca ccggagtagc gcgagcacag ctagtttaag
      361 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
      421 agtggcaccg agtcggtgct ttttttgaat tcgctagcta ggtcttgaaa ggagtgggaa
      481 ttgtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      841 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      901 catctccccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
      961 cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     1021 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1081 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1141 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1201 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     1261 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     1321 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     1381 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     1441 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1501 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1561 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1621 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     1681 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     1741 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     1801 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     1861 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     1921 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1981 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2041 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2101 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2161 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgggc
     2221 cgcgttgacg cgcacggcaa gaggcgaggg gcggcgactg gtgagagatg ggtgcgagag
     2281 cgtcagtatt aagcggggga gaattagatc gatgggaaaa aattcggtta aggccagggg
     2341 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     2401 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     2461 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     2521 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     2581 aggaagagca aaacaaaagt aagaaaaaag cacagcaagc agcagctgac acaggacaca
     2641 gcaatcaggt cagccaaaat taccctatag tgcagaacat ccaggggcaa atggtacatc
     2701 aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca
     2761 gcccagaagt gatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa
     2821 acaccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca
     2881 tcaatgagga agctgcagaa tgggatagag tgcatccagt gcatgcaggg cctattgcac
     2941 caggccagat gagagaacca aggggaagtg acatagcagg aactactagt acccttcagg
     3001 aacaaatagg atggatgaca cataatccac ctatcccagt aggagaaatc tataaaagat
     3061 ggataatcct gggattaaat aaaatagtaa gaatgtatag ccctaccagc attctggaca
     3121 taagacaagg accaaaggaa ccctttagag actatgtaga ccgattctat aaaactctaa
     3181 gagccgagca agcttcacaa gaggtaaaaa attggatgac agaaaccttg ttggtccaaa
     3241 atgcgaaccc agattgtaag actattttaa aagcattggg accaggagcg acactagaag
     3301 aaatgatgac agcatgtcag ggagtggggg gacccggcca taaagcaaga gttttggctg
     3361 aagcaatgag ccaagtaaca aatccagcta ccataatgat acagaaaggc aattttagga
     3421 accaaagaaa gactgttaag tgtttcaatt gtggcaaaga agggcacata gccaaaaatt
     3481 gcagggcccc taggaaaaag ggctgttgga aatgtggaaa ggaaggacac caaatgaaag
     3541 attgtactga gagacaggct aattttttag ggaagatctg gccttcccac aagggaaggc
     3601 cagggaattt tcttcagagc agaccagagc caacagcccc accagaagag agcttcaggt
     3661 ttggggaaga gacaacaact ccctctcaga agcaggagcc gatagacaag gaactgtatc
     3721 ctttagcttc cctcagatca ctctttggca gcgacccctc gtcacaataa agataggggg
     3781 gcaattaaag gaagctctat tagatacagg agcagatgat acagtattag aagaaatgaa
     3841 tttgccagga agatggaaac caaaaatgat agggggaatt ggaggtttta tcaaagtaag
     3901 acagtatgat cagatactca tagaaatctg cggacataaa gctataggta cagtattagt
     3961 aggacctaca cctgtcaaca taattggaag aaatctgttg actcagattg gctgcacttt
     4021 aaattttccc attagtccta ttgagactgt accagtaaaa ttaaagccag gaatggatgg
     4081 cccaaaagtt aaacaatggc cattgacaga agaaaaaata aaagcattag tagaaatttg
     4141 tacagaaatg gaaaaggaag gaaaaatttc aaaaattggg cctgaaaatc catacaatac
     4201 tccagtattt gccataaaga aaaaagacag tactaaatgg agaaaattag tagatttcag
     4261 agaacttaat aagagaactc aagatttctg ggaagttcaa ttaggaatac cacatcctgc
     4321 agggttaaaa cagaaaaaat cagtaacagt actggatgtg ggcgatgcat atttttcagt
     4381 tcccttagat aaagacttca ggaagtatac tgcatttacc atacctagta taaacaatga
     4441 gacaccaggg attagatatc agtacaatgt gcttccacag ggatggaaag gatcaccagc
     4501 aatattccag tgtagcatga caaaaatctt agagcctttt agaaaacaaa atccagacat
     4561 agtcatctat caatacatgg atgatttgta tgtaggatct gacttagaaa tagggcagca
     4621 tagaacaaaa atagaggaac tgagacaaca tctgttgagg tggggattta ccacaccaga
     4681 caaaaaacat cagaaagaac ctccattcct ttggatgggt tatgaactcc atcctgataa
     4741 atggacagta cagcctatag tgctgccaga aaaggacagc tggactgtca atgacataca
     4801 gaaattagtg ggaaaattga attgggcaag tcagatttat gcagggatta aagtaaggca
     4861 attatgtaaa cttcttaggg gaaccaaagc actaacagaa gtagtaccac taacagaaga
     4921 agcagagcta gaactggcag aaaacaggga gattctaaaa gaaccggtac atggagtgta
     4981 ttatgaccca tcaaaagact taatagcaga aatacagaag caggggcaag gccaatggac
     5041 atatcaaatt tatcaagagc catttaaaaa tctgaaaaca ggaaagtatg caagaatgaa
     5101 gggtgcccac actaatgatg tgaaacaatt aacagaggca gtacaaaaaa tagccacaga
     5161 aagcatagta atatggggaa agactcctaa atttaaatta cccatacaaa aggaaacatg
     5221 ggaagcatgg tggacagagt attggcaagc cacctggatt cctgagtggg agtttgtcaa
     5281 tacccctccc ttagtgaagt tatggtacca gttagagaaa gaacccataa taggagcaga
     5341 aactttctat gtagatgggg cagccaatag ggaaactaaa ttaggaaaag caggatatgt
     5401 aactgacaga ggaagacaaa aagttgtccc cctaacggac acaacaaatc agaagactga
     5461 gttacaagca attcatctag ctttgcagga ttcgggatta gaagtaaaca tagtgacaga
     5521 ctcacaatat gcattgggaa tcattcaagc acaaccagat aagagtgaat cagagttagt
     5581 cagtcaaata atagagcagt taataaaaaa ggaaaaagtc tacctggcat gggtaccagc
     5641 acacaaagga attggaggaa atgaacaagt agataaattg gtcagtgctg gaatcaggaa
     5701 agtactattt ttagatggaa tagataaggc ccaagaagaa catgagaaat atcacagtaa
     5761 ttggagagca atggctagtg attttaacct accacctgta gtagcaaaag aaatagtagc
     5821 cagctgtgat aaatgtcagc taaaagggga agccatgcat ggacaagtag actgtagccc
     5881 aggaatatgg cagctagatt gtacacattt agaaggaaaa gttatcttgg tagcagttca
     5941 tgtagccagt ggatatatag aagcagaagt aattccagca gagacagggc aagaaacagc
     6001 atacttcctc ttaaaattag caggaagatg gccagtaaaa acagtacata cagacaatgg
     6061 cagcaatttc accagtacta cagttaaggc cgcctgttgg tgggcgggga tcaagcagga
     6121 atttggcatt ccctacaatc cccaaagtca aggagtaata gaatctatga ataaagaatt
     6181 aaagaaaatt ataggacagg taagagatca ggctgaacat cttaagacag cagtacaaat
     6241 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     6301 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     6361 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggaaaggacc
     6421 agcaaagctc ctctggaaag gtgaaggggc agtagtaata caagataata gtgacataaa
     6481 agtagtgcca agaagaaaag caaagatcat cagggattat ggaaaacaga tggcaggtga
     6541 tgattgtgtg gcaagtagac aggatgagga ttaacacatg gaattctgca acaactgctg
     6601 tttatccatt tcagaattgg gttcgacata gcagaatagg cgttactcga cagaggagag
     6661 caagaaatgg agccagtaga tcctagacta gagccctgga agcatccagg aagtcagcct
     6721 aaaactgctt gtaccaattg ctattgtaaa aagtgttgct ttcattgcca agtttgtttc
     6781 atgacaaaag ccttaggcat ctcctatggc aggaagaagc ggagacagcg acgaagagct
     6841 catcagaaca gtcagactca tcaagcttct ctatcaaagc agtaagtagt acatgtaatg
     6901 caacctataa tagtagcaat agtagcatta gtagtagcaa taataatagc aatagttgtg
     6961 tggtccatag taatcataga atataggaaa atggccgctg atcttcagac ctggaggagg
     7021 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7081 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7141 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7201 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7261 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7321 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7381 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7441 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7501 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7561 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7621 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     7681 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     7741 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     7801 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcctt
     7861 ggcacttatc tgggacgatc tgcggagcct gtgcctcttc agctaccacc gcttgagaga
     7921 cttactcttg attgtaacga ggattgtgga acttctggga cgcagggggt gggaagccct
     7981 caaatattgg tggaatctcc tacaatattg gagtcaggag ctaaagaata gtgctgttag
     8041 cttgctcaat gccacagcca tagcagtagc tgaggggaca gatagggtta tagaagtagt
     8101 acaaggagct tgtagagcta ttcgccacat acctagaaga ataagacagg gcttggaaag
     8161 gattttgcta taagctcgaa acaaccggta cctctagaac tatagctagc agatcttttt
     8221 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     8281 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     8341 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     8401 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     8461 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     8521 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     8581 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     8641 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     8701 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8761 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8821 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
     8881 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9001 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9061 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9121 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9181 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
     9241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11161 tccgcg
//
