LOCUS       Exported               11799 bp ds-DNA     circular SYN 04-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3862..5532
                     /organism="Homo sapiens"
                     /chromosome="16"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     enhancer        232..611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        613..811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             829..1009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2434..2551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2661..3838
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2892..3829
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     gene            3862..5532
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /label=CD19
                     /note="CD19 molecule; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
     mRNA            join(3862..3949,4217..5532)
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="CD19 molecule, transcript variant X1"
                     /label=CD19 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 11 ESTs, 3 long SRA reads, and 100%
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 20 samples with support for all 
                     annotated introns"
                     /note="/transcript_id=XM_006721103.3"
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
     mRNA            3862..5532
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="CD19 molecule, transcript variant 1"
                     /label=CD19 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_001178098.2"
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
     mRNA            join(3862..3949,4217..5532)
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="CD19 molecule, transcript variant 3"
                     /label=CD19 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_001385732.1"
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
     CDS             3862..5532
                     /codon_start=1
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="B-lymphocyte antigen CD19 isoform 1 precursor"
                     /label=CD19
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; Derived by automated computational analysis 
                     using gene prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS53998.1"
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
                     /protein_id="NP_001171569.1"
                     /translation="MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDG
                     PTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSE
                     KAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDR
                     PEIWEGEPPCVPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSASRGPLSWTHVHPKGP
                     KSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLW
                     HWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPP
                     GSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGV
                     GPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNA
                     ESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIR
                     GQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR"
     CDS             join(3862..3949,4217..5532)
                     /codon_start=1
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="B-lymphocyte antigen CD19 isoform X1"
                     /label=CD19
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
                     /protein_id="XP_006721166.1"
                     /translation="MPPPRLLFFLLFLTPMEVRPEEPLVVKVEGELFRWNVSDLGGLGC
                     GLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCVPPRDSLNQSLSQDLTMAPG
                     STLWLSCGVPPDSASRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRAT
                     AQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGIL
                     HLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAG
                     LGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQ
                     DQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDP
                     SREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGG
                     GRMGTWSTR"
     CDS             join(3862..3949,4217..5532)
                     /codon_start=1
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="B-lymphocyte antigen CD19 isoform 3 precursor"
                     /label=CD19
                     /note="isoform 3 precursor is encoded by transcript
                     variant 3; Derived by automated computational analysis 
                     using gene prediction method: BestRefSeq."
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
                     /protein_id="NP_001372661.1"
                     /translation="MPPPRLLFFLLFLTPMEVRPEEPLVVKVEGELFRWNVSDLGGLGC
                     GLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCVPPRDSLNQSLSQDLTMAPG
                     STLWLSCGVPPDSASRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRAT
                     AQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGIL
                     HLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAG
                     LGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQ
                     DQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDP
                     SREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGG
                     GRMGTWSTR"
     misc_RNA        join(3862..4807,4857..5034)
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="CD19 molecule, transcript variant X3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 54 ESTs, 1 long SRA read, and 100% 
                     coverage of the annotated genomic feature by RNAseq 
                     alignments, including 20 samples with support for all 
                     annotated introns"
                     /note="/transcript_id=XR_950871.2"
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
     mRNA            3862..4768
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="CD19 molecule, transcript variant X4"
                     /label=CD19 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 52 ESTs, and 100% coverage of the 
                     annotated genomic feature by RNAseq alignments, including 
                     18 samples with support for all annotated introns"
                     /note="/transcript_id=XM_011545981.2"
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
     CDS             3862..4696
                     /codon_start=1
                     /gene="CD19"
                     /gene_synonym="B4; CVID3"
                     /product="B-lymphocyte antigen CD19 isoform X3"
                     /label=CD19
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /db_xref="GeneID:930"
                     /db_xref="HGNC:HGNC:1633"
                     /db_xref="MIM:107265"
                     /protein_id="XP_011544283.1"
                     /translation="MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDG
                     PTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSE
                     KAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDR
                     PEIWEGEPPCVPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSASRGPLSWTHVHPKGP
                     KSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARP"
     misc_feature    5570..6143
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6157..6873
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    6891..7479
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             7551..7784
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7816..8040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8086..8514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8528..8857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8708..8843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8905..8952
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8971..9345
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9475..9596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9645..9661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9669..9685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9693..9723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9738..9759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10047..10635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10806..11666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11667..11771)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat tgacattgat
      241 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      301 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      361 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      421 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      481 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      541 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      601 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      661 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      721 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      781 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
      841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2401 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2581 agtttggtta attaaggtac catcgatctt aagtcgcgac tcgagaattc gctagctagg
     2641 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2701 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     2761 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2821 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2881 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2941 gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg atcccgagct
     3001 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3061 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3121 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3181 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3241 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3301 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3361 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3421 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3481 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3541 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3601 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3661 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3721 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3781 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaac
     3841 cggttctaga gcgctgccac catgccacct cctcgcctcc tcttcttcct cctcttcctc
     3901 acccccatgg aagtcaggcc cgaggaacct ctagtggtga aggtggaaga gggagataac
     3961 gctgtgctgc agtgcctcaa ggggacctca gatggcccca ctcagcagct gacctggtct
     4021 cgggagtccc cgcttaaacc cttcttaaaa ctcagcctgg ggctgccagg cctgggaatc
     4081 cacatgaggc ccctggccat ctggcttttc atcttcaacg tctctcaaca gatggggggc
     4141 ttctacctgt gccagccggg gcccccctct gagaaggcct ggcagcctgg ctggacagtc
     4201 aatgtggagg gcagcgggga gctgttccgg tggaatgttt cggacctagg tggcctgggc
     4261 tgtggcctga agaacaggtc ctcagagggc cccagctccc cttccgggaa gctcatgagc
     4321 cccaagctgt atgtgtgggc caaagaccgc cctgagatct gggagggaga gcctccgtgt
     4381 gtcccaccga gggacagcct gaaccagagc ctcagccagg acctcaccat ggcccctggc
     4441 tccacactct ggctgtcctg tggggtaccc cctgactctg cgtccagggg ccccctctcc
     4501 tggacccatg tgcaccccaa ggggcctaag tcattgctga gcctagagct gaaggacgat
     4561 cgcccggcca gagatatgtg ggtaatggag acgggtctgt tgttgccccg ggccacagct
     4621 caagacgctg gaaagtatta ttgtcaccgt ggcaacctga ccatgtcatt ccacctggag
     4681 atcactgctc ggccagtact atggcactgg ctgctgagga ctggtggctg gaaggtctca
     4741 gctgtgactt tggcttatct gatcttctgc ctgtgttccc ttgtgggcat tcttcatctt
     4801 caaagagccc tggtcctgag gaggaaaaga aagcgaatga ctgaccccac caggagattc
     4861 ttcaaagtga cgcctccccc aggaagcggg ccccagaacc agtacgggaa cgtgctgtct
     4921 ctccccacac ccacctcagg cctcggacgc gcccagcgtt gggccgcagg cctggggggc
     4981 actgccccgt cttatggaaa cccgagcagc gacgtccagg cggatggagc cttggggtcc
     5041 cggagcccgc cgggagtggg cccagaagaa gaggaagggg agggctatga ggaacctgac
     5101 agtgaggagg actccgagtt ctatgagaac gactccaacc ttgggcagga ccagctctcc
     5161 caggatggca gcggctacga gaaccctgag gatgagcccc tgggtcctga ggatgaagac
     5221 tccttctcca acgctgagtc ttatgagaac gaggatgaag agctgaccca gccagtcgcc
     5281 aggacaatgg acttcctgag ccctcatggg tcagcctggg accccagccg ggaagcaacc
     5341 tccctggggt cccagtccta tgaggatatg agaggaatcc tgtatgcagc cccccagctc
     5401 cgctccattc ggggccagcc tggacccaat catgaggaag atgcagactc ttatgagaac
     5461 atggataatc ccgatgggcc agacccagcc tggggaggag ggggccgcat gggcacctgg
     5521 agcaccaggt gaacgcgtta agtcgacaaa tgacaagtaa gcggccgcgc ccctctccct
     5581 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5641 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5701 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5761 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5821 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggcccaa
     5881 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5941 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     6001 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     6061 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     6121 tcctttgaaa aacacgatga taatatggcc acacatatgg tgagcaaggg cgaggagctg
     6181 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6241 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6301 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     6361 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6421 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6481 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6541 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6601 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6661 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6721 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     6781 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6841 gggatcactc tcggcatgga cgagctgtac aagtagcgcg ttaagtcgac aatcaacctc
     6901 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6961 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7021 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7081 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7141 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7201 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7261 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7321 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7381 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7441 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     7501 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7561 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7621 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7681 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7741 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7801 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7861 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7921 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7981 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8041 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8101 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8161 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8281 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8401 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8461 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8521 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8581 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8641 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8701 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8761 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8821 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8881 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8941 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     9001 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     9061 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     9121 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     9181 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     9241 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     9301 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     9361 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     9421 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     9481 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9541 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9601 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
