LOCUS       Exported               11505 bp ds-DNA     circular SYN 04-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        232..611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        613..811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             829..1009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2434..2551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2661..3838
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2892..3829
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             3862..5235
                     /codon_start=1
                     /label=Human CD4
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRHRRRQAERMSQIKRLLSEKKTCQCPHRFQKTCSPI
                     "
     misc_feature    5276..5849
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5863..6579
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    6597..7185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             7257..7490
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7522..7746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7792..8220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8234..8563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8414..8549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8611..8658
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8677..9051
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9181..9302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9351..9367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9375..9391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9399..9429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9444..9465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9753..10341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10512..11372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11373..11477)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat tgacattgat
      241 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      301 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      361 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      421 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      481 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      541 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      601 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      661 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      721 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      781 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
      841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2401 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2581 agtttggtta attaaggtac catcgatctt aagtcgcgac tcgagaattc gctagctagg
     2641 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2701 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     2761 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2821 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2881 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2941 gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg atcccgagct
     3001 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3061 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3121 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3181 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3241 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3301 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3361 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3421 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3481 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3541 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3601 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3661 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3721 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3781 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaac
     3841 cggttctaga gcgctgccac catgaacagg ggagtgccat ttcgccatct gctcctggtg
     3901 ttgcagctcg cgctccttcc ggcggctacc cagggtaaaa aggtagtcct cggaaaaaaa
     3961 ggcgacacag tggaacttac ctgcaccgcg agccaaaaga agtctattca gtttcattgg
     4021 aagaactcaa atcagatcaa gatacttggc aaccaaggat cttttctgac taagggacct
     4081 tcaaaactta atgacagggc agattctagg agatctttgt gggaccaagg gaattttcct
     4141 cttattatca aaaatctcaa gatagaggac agtgacacat atatctgtga ggtggaagat
     4201 caaaaggagg aagtgcaact gctcgtcttc gggctgacag ccaacagcga tacgcacttg
     4261 cttcaagggc agtctcttac ccttaccttg gagtctccac ccgggtcatc cccttcagtt
     4321 cagtgccgga gcccgcgcgg taagaacata caaggaggca agacgttgtc tgtgtcccag
     4381 cttgagctcc aagatagcgg gacctggacg tgtacagtcc tccagaatca aaagaaggta
     4441 gagtttaaaa tcgatatagt cgtcctcgcc ttccagaagg ctagttccat agtatataaa
     4501 aaggaaggcg agcaagtcga gtttagtttt cccctggcgt ttaccgtcga gaagttgact
     4561 ggtagcggtg agctttggtg gcaagctgag agggcaagtt cttcaaaatc ctggatcaca
     4621 tttgatctca agaataaaga ggttagcgtt aaacgggtga cgcaagaccc gaaactccaa
     4681 atgggaaaga aattgcctct gcaccttact cttccgcaag ctcttcccca atacgctggt
     4741 agtggtaatt tgaccttggc actggaagca aaaacaggca agctccacca ggaggtaaat
     4801 ttggtagtta tgcgcgcgac tcaacttcaa aagaacttga cttgcgaggt ttgggggccc
     4861 acgagcccaa agcttatgct ttctctgaag cttgagaata aagaggccaa ggtcagtaag
     4921 cgagaaaagg cggtgtgggt tttgaacccc gaagcaggga tgtggcaatg cctcctcagc
     4981 gactccggtc aagtactctt ggaatccaac atcaaggttc tccccacctg gagtacgcca
     5041 gtacagccta tggcactcat tgtactgggg ggcgttgcgg gtctcttgct ttttatcggt
     5101 ttgggtatct tcttttgtgt aaggtgtcgg catagacggc gacaggcaga acgcatgtcc
     5161 caaataaaac gccttctctc tgaaaagaag acctgccaat gcccgcatag attccagaag
     5221 acttgctccc caatataaac gcgttaagtc gacaaatgac aagtaagcgg ccgcgcccct
     5281 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5341 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5401 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5461 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5521 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5581 gcccaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5641 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5701 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5761 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5821 tggttttcct ttgaaaaaca cgatgataat atggccacac atatggtgag caagggcgag
     5881 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5941 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     6001 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     6061 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     6121 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     6181 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     6241 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     6301 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     6361 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6421 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6481 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6541 gccgccggga tcactctcgg catggacgag ctgtacaagt agcgcgttaa gtcgacaatc
     6601 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6661 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6721 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6781 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6841 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6901 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6961 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7021 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7081 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7141 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     7201 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7261 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7321 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7381 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7441 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     7501 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7561 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7621 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7681 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7741 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     7801 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7861 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7921 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7981 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8041 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8101 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8161 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8221 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8281 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8341 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8401 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8461 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     8521 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     8581 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     8641 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     8701 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     8761 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     8821 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     8881 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     8941 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     9001 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     9061 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9121 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     9181 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9241 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9301 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9361 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9421 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9481 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9541 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10501 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10561 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10621 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10681 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10741 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10801 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10861 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10921 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10981 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11041 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11101 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11161 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11221 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11281 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11341 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11401 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11461 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
