LOCUS       pAG416GAL-1x-HIV        5843 bp DNA     circular SYN 28-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2023 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             771..864
                     /locus_tag="lacI"
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
     protein_bind    complement(877..898)
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     promoter        913..943
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(947..969)
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     promoter        1012..1030
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1048..1401
                     /locus_tag="GAL1 promoter"
                     /label=GAL1 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=99.718"
                     /note="/match_length=80.31674208144797"
                     /note="/other=promoter"
     misc_feature    1416..1436
                     /locus_tag="MCS(1)"
                     /label=MCS
                     /label=MCS(1)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.81132075471698"
                     /note="/other=misc feature"
     misc_feature    1440..1464
                     /locus_tag="attB1"
                     /label=attB1
     misc_feature    1470..1772
                     /locus_tag="HIV-2 PR"
                     /label=HIV-2 PR
     misc_feature    1774..1798
                     /locus_tag="attB2"
                     /label=attB2
     misc_feature    1802..1849
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=45.28301886792453"
                     /note="/other=misc feature"
     terminator      1850..2097
                     /locus_tag="CYC1 terminator"
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     promoter        complement(2116..2134)
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2142..2300
                     /locus_tag="lacZ"
                     /label=lacZ
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.170731707317073"
                     /note="/other=CDS"
     rep_origin      2301..2756
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(2757..2809)
                     /locus_tag="bom"
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.1294964028777"
                     /note="/other=misc feature"
     CDS             complement(2887..3690)
                     /locus_tag="URA3"
                     /label=URA3
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(3691..3906)
                     /locus_tag="URA3 promoter"
                     /label=URA3 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(3914..3963)
                     /locus_tag="bom(1)"
                     /label=bom
                     /label=bom(1)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.97122302158273"
                     /note="/other=misc feature"
     CDS             complement(4065..4099)
                     /locus_tag="rop"
                     /label=rop
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.047619047619047"
                     /note="/other=CDS"
     CDS             complement(4100)
                     /locus_tag="rop(1)"
                     /label=rop
                     /label=rop(1)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.047619047619047"
                     /note="/other=CDS"
     misc_feature    4168..4671
                     /locus_tag="CEN/ARS"
                     /label=CEN/ARS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.802"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     promoter        4708..4812
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4813..5673
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcgc agatgtgcct cgcgccgcac tgctccgaac
     1081 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     1141 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     1201 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     1261 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     1321 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     1381 ctatacttta acgtcaagga gaaaaaaccc cggattctag aactagtgga tcccccatca
     1441 caagtttgta caaaaaagca ggctacaaaa tgccgcaatt ctccctgtgg aaaaggcctg
     1501 ttgtgaccgc gtatatcgag gggcaaccag ttgaagttct cctggacact ggggctgatg
     1561 actccatcgt cgcaggtatt gaattgggaa ataactactc ccctaagatt gtggggggca
     1621 taggagggtt catcaacaca aaggagtaca agaatgttga aatcgaggta ttgaacaaaa
     1681 aagtcagagc aaccattatg acaggcgata cgccaatcaa catatttggt cgaaacatct
     1741 tgacggcttt gggtatgtca ctcaatctct aaaacccagc tttcttgtac taagtggtga
     1801 tgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagt catgtaatta
     1861 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
     1921 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
     1981 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
     2041 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc
     2101 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     2161 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2221 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2281 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2341 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2401 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2461 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2521 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2581 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2641 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2701 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     2761 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg
     2821 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
     2881 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
     2941 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
     3001 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
     3061 tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct
     3121 tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat
     3181 gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg acaattctgc
     3241 taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct
     3301 aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct
     3361 gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc
     3421 ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc
     3481 catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa
     3541 tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt
     3601 tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc
     3661 agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt
     3721 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
     3781 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     3841 cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     3901 attgaaaagg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3961 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4021 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4081 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4141 gtcatgataa taatggtttc ttagacggat cgcttgcctg taacttacac gcgcctcgta
     4201 tcttttaatg atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt
     4261 atttggattt tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa
     4321 taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca
     4381 agaaaagcag attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag
     4441 gattttcgtg tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag
     4501 agcaggaaga gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat
     4561 cttcggaaaa caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt
     4621 atatatttat attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac
     4681 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5341 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5821 gtagaaaaga tcaaaggatc ttc
//
