LOCUS       Exported               11029 bp ds-DNA     circular SYN 27-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   acharyasundaram
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 3, 2023 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5110..5823
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             5833..5853
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5860..5886
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5890..5910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5920..10803
                     /codon_start=1
                     /product="Cas9 endonuclease from the Francisella novicida 
                     Type II CRISPR/Cas system (Hirano et al., 2016)"
                     /label=en15dFnCas9
                     /translation="NFKILPIAIALGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKD
                     SYTLLMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFNRRGFS
                     FITDGYSPEYLNIVPEQVKAILMDIFDDYNGEDDLDSYLKLATEQESKISEIYNKLMQK
                     ILEFKLMKLCTDIKDDKVSTKTLKEITSYEFELLADYLANYSESLKTQKFSYTDKQGNL
                     KELSYYHHDKYNIQEFLKRHATINDRILDTLLTDDLDIWNFNFEKFDFDKNEEKLQNQE
                     DKDHIQAHLHHFVFAVNKIKSEMASGGRHRSQYFQEITNVLDENNHQEGYLKNFCENLH
                     NKKYSNLSVKNLVNLIGNLSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEW
                     RVGVKDQDKKDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPK
                     CQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKDQPYFVEYKS
                     SNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQAKKLKQKASSELEKLESS
                     KKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQRQRARDSRLYIMPEYRYDK
                     KLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQLLNDLAGVLQVSPNFLKDKIGSDDDLFI
                     SKWLVEHIRGFKKACEDSLKIQKDNRGLLNHKINIARNTKGKCEKEIFNLICKIEGSED
                     KKGNYKHGLAYELGVLLFGEPNEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANT
                     CAVCSADNAHRMQQIKITEPVEDNKDKIILSAKAQRLPAIPTRIVDGAVKKMATILAKN
                     IVDDNWQNIKQVLSAKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPEN
                     IFKDKNNRIKEFAKGISAYSGANLTDGDFDGAKEELDAIIPRSHKKYGTLNDEANLICV
                     TRGDNKNKGNRIFCLRDLADNYKLKQFETTDDLEIEKKIADTIWDANKKDFKFGNYRSF
                     INLTPQEQKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLRAK
                     KENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKPQASYSHLIDAM
                     LAFCIAADEHRNDGSIGLEIDKNYSLYPLDKNTGEVFTKDIFSQIKITDNEFSDKKLVR
                     KKAIEGFNTHRQMTRDGIYAENYLPILIHKELNEVRKGYTWKNSEEIKIFKGKKYDIQQ
                     LNNLVYCLKFVDKPISIDIQISTLEELRNILTTNNIAATAEYYYINLKTQKLHEYYIEN
                     YNTALGYKKYSKEMEFLRSLAYRSERVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQ
                     RLYREWQNTTIKDDYEFLKSFFNVKSITKLHKKVRKDFSLPISTNEGKFLVKRKTWDNN
                     FIYQILNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKVDNKN
                     IFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVIDDDSKINYFMNHS
                     LLKSRYPDKVLEILKQSTIIEFHSSGFNKTIKEMLGMKLAGIYNETSNN"
     CDS             10873..10890
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      10957..11004
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaact agtaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatgt
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggttctt ctcaccatca ccatcaccat
     5101 ggttcttctg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5161 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     5221 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5281 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5341 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5401 atctccttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     5461 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5521 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcacggc cgacaagcag
     5581 aagaacggca tcaaggcgaa cttcaagatc cgccacaacg tcgaggacgg cagcgtgcag
     5641 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5701 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     5761 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5821 aaggggatcg aggaaaacct gtacttccaa tccaatgcat acccatacga tgttccagat
     5881 tacgcttcgc cgaagaaaaa gcgcaaggtc gaagcgtcca attttaagat cctgcctatc
     5941 gcaatcgccc tgggcgtcaa gaatactggc gtgtttagtg ctttttatca gaaggggacc
     6001 tcactggaga gactggacaa taagaacgga aaagtgtatg aactgtccaa ggattcttac
     6061 actctgctga tgaacaatag gaccgcacgg agacaccaga ggcgaggaat tgacaggaaa
     6121 cagctggtga agcgcctgtt caaactgatc tggacagagc agctgaacct ggaatgggat
     6181 aaggacactc agcaggccat cagcttcctg tttaatcgac ggggattctc ttttattact
     6241 gacggctata gtcctgagta cctgaacatc gtgccagaac aggtcaaggc aatcctgatg
     6301 gacattttcg acgattataa tggcgaggac gatctggatt cctacctgaa actggccaca
     6361 gagcaagaga gtaagatcag cgaaatctac aacaagctga tgcagaagat cctggagttc
     6421 aagctgatga aactgtgcac cgacatcaag gacgataaag tgagtaccaa gacactgaaa
     6481 gagatcacaa gctacgagtt cgaactgctg gccgattatc tggctaacta cagcgaatcc
     6541 ctgaagaccc agaaattttc ctacacagac aagcagggca atctgaaaga gctgtcttac
     6601 taccaccatg ataagtacaa catccaggag ttcctgaaga gacacgccac catcaatgac
     6661 aggattctgg atacactgct gactgacgat ctggacatct ggaacttcaa cttcgagaag
     6721 ttcgatttcg acaagaacga ggaaaaactg cagaatcagg aagataagga ccacattcag
     6781 gctcatctgc accatttcgt gtttgcagtc aataagatca aaagcgagat ggcatccggc
     6841 gggcgccatc gaagccagta cttccaggaa atcaccaacg tgctggacga gaacaatcac
     6901 caggaaggct acctgaaaaa cttctgtgag aatctgcata acaagaagta cagcaatctg
     6961 tccgtgaaga atctggtcaa cctgattgga aatctgtcca acctggaact gaagcccctg
     7021 cgcaaatact tcaacgacaa gatccacgct aaagcagacc attgggatga gcagaagttt
     7081 actgaaacct attgccactg gattctgggc gagtggcggg tgggggtcaa ggatcaggac
     7141 aagaaagacg gcgcaaagta ttcttacaag gacctgtgta acgagctgaa gcagaaagtg
     7201 actaaggccg ggctggtgga cttcctgctg gagctggacc cctgccgaac cattccacct
     7261 tacctggaca acaataacag aaagccaccc aaatgtcaga gcctgatcct gaatcccaag
     7321 tttctggata atcagtatcc taactggcag cagtacctgc aggagctgaa gaaactgcag
     7381 tcaatccaga actacctgga cagcttcgaa accgatctga aggtgctgaa aagctccaag
     7441 gaccagcctt acttcgtcga gtacaagtct agtaaccagc agatcgcttc cggccagcgg
     7501 gattacaagg atctggacgc aagaatcctg cagttcattt ttgacagggt gaaggcctct
     7561 gatgagctgc tgctgaacga aatctatttc caggcaaaga aactgaagca gaaagcctca
     7621 agcgagctgg aaaagctgga gtcctctaag aaactggacg aagtgatcgc taactctcag
     7681 ctgagtcaga ttctgaagtc tcagcacaca aatggaatct tcgagcaggg cacttttctg
     7741 catctggtgt gcaaatacta taagcagcga cagagagcca gggacagccg cctgtacatc
     7801 atgcctgaat atcgatacga taagaaactg cacaagtaca acaacaccgg ccgctttgac
     7861 gatgacaacc agctgctgac atattgtaat cataagcccc ggcagaaaag ataccagctg
     7921 ctgaacgacc tggcaggagt gctgcaggtc tctcctaatt ttctgaagga taaaatcggg
     7981 tccgatgacg atctgttcat ttctaagtgg ctggtggagc acatccgggg ctttaagaag
     8041 gcctgcgaag acagcctgaa aatccagaag gataacaggg gactgctgaa tcataagatc
     8101 aacattgcac gcaataccaa gggcaaatgc gagaaagaaa tcttcaacct gatctgtaag
     8161 attgagggga gcgaagacaa gaaagggaat tataagcacg gactggccta cgagctggga
     8221 gtgctgctgt tcggagagcc aaacgaggcc agcaagcccg aatttgatag gaaaatcaag
     8281 aaattcaatt caatctacag ctttgcccag atccagcaga ttgcctttgc tgagaggaag
     8341 gggaatgcaa acacatgcgc cgtgtgtagt gcagacaacg cccatcgcat gcagcagatc
     8401 aaaattactg agccagtcga agacaataag gataaaatca ttctgtcagc aaaggcacag
     8461 cgactgcctg caatcccaac ccgaattgtg gatggagctg tcaagaaaat ggctacaatt
     8521 ctggcaaaga atatcgtgga cgataattgg cagaacatta agcaggtcct gagcgcaaaa
     8581 caccagctgc atatcccaat cattaccgag tccaacgcct tcgagtttga acccgctctg
     8641 gcagacgtga agggcaaatc tctgaaggat agaaggaaga aagccctgga gcgaattagt
     8701 cccgaaaaca tcttcaagga taagaacaac agaatcaagg agtttgctaa ggggatttcc
     8761 gcctactctg gagctaacct gacagatggg gacttcgatg gagcaaagga ggaactggat
     8821 gccatcattc ctcgcagcca taagaaatat ggcactctga acgacgaggc taatctgatt
     8881 tgcgtgaccc ggggcgataa taagaacaaa gggaaccgga tcttctgtct gagagacctg
     8941 gccgataatt acaagctgaa acagtttgag accacagacg atctggagat cgaaaagaaa
     9001 attgccgaca ccatctggga tgctaataag aaggacttca agttcggaaa ctatcggagc
     9061 ttcatcaatc tgacacctca ggagcagaaa gcattcagac acgccctgtt tctggctgat
     9121 gaaaacccaa tcaagcaggc agtgatcaga gccattaata accgcaaccg aaccttcgtg
     9181 aatggcacac agaggtattt tgctgaggtc ctggcaaata acatctacct gcgcgccaag
     9241 aaagaaaatc tgaacactga caagatcagc ttcgattact ttggaatccc taccattgga
     9301 aacggccgag ggatcgctga gattcggcag ctgtatgaaa aggtggacag tgatatccag
     9361 gcctacgcta aaggcgacaa gccacaggcc tcttatagtc acctgattga tgctatgctg
     9421 gcattctgca tcgccgctga cgagcatcgg aacgatggat ctattggcct ggaaatcgac
     9481 aaaaactata gtctgtaccc tctggataag aatactggcg aggtgttcac caaagacatc
     9541 ttttcacaga tcaagattac cgacaacgag ttcagcgata agaaactggt cagaaagaaa
     9601 gctattgaag ggtttaacac acacagacag atgactaggg atggaatcta tgcagagaat
     9661 tacctgccta tcctgattca taaggagctg aacgaagtga ggaaggggta cacatggaaa
     9721 aattccgagg aaatcaaaat tttcaaggga aagaaatacg acatccagca gctgaataac
     9781 ctggtgtatt gtctgaagtt tgtggacaaa ccaatcagta ttgatatcca gatttcaacc
     9841 ctggaggaac tgagaaacat cctgactacc aataacattg cagccactgc cgagtactat
     9901 tacattaatc tgaaaaccca gaagctgcac gagtattaca tcgaaaatta caacacagcc
     9961 ctggggtata agaaatacag caaggagatg gagttcctga ggtccctggc ttataggtct
    10021 gagcgcgtga agatcaaaag tattgacgat gtcaagcagg tcctggacaa ggattcaaac
    10081 ttcatcatcg gaaagatcac actgcccttc aagaaagagt ggcagcgact gtaccgggaa
    10141 tggcagaaca caactatcaa agacgattat gagtttctga agagcttctt taatgtgaag
    10201 tccattacta aactgcacaa gaaagtccgg aaagacttct ctctgcccat cagtacaaac
    10261 gagggcaagt ttctggtgaa gagaaaaact tgggataata acttcatcta ccagattctg
    10321 aatgactcag atagcagggc agacgggact aaacccttca ttcctgcctt tgatatcagc
    10381 aagaacgaga ttgtggaagc catcattgac agtttcacct caaaaaacat cttttggctg
    10441 ccaaagaata ttgagctgca gaaggtggac aacaagaaca tcttcgccat tgataccagc
    10501 aagtggtttg aggtcgaaac accatccgac ctgcgcgata tcggcattgc taccattcag
    10561 tacaagatcg acaataactc acgccccaag gtgcgagtca aactggatta cgtgatcgac
    10621 gatgacagca agattaacta tttcatgaat cactcactgc tgaagagccg gtatcccgac
    10681 aaagtcctgg agatcctgaa gcagagcaca atcattgagt tccacagttc agggtttaac
    10741 aaaactatta aggagatgct gggaatgaag ctggccggca tctacaatga aacctccaat
    10801 aactaataac attggaagtg gataacggat ccgaattcga gcgccgtcga caagcttgcg
    10861 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
    10921 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
    10981 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
//
