LOCUS       Exported               11308 bp ds-DNA     circular SYN 28-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   acharyasundaram
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 3, 2023 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        268..340
                     /label=Fn gRNA scaffold
                     /note="guide RNA scaffold for the Francisella novicida 
                     CRISPR/Cas9 system (Hirano et al., 2016)"
     enhancer        437..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        724..1001
                     /label=chicken beta-actin promoter
     intron          1002..1230
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1242..1251
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    1257..1310
                     /label=bpNLS
     CDS             1290..1310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1311..1808
                     /label=WT TAD
     misc_feature    1809..1904
                     /label=32 aa linker
     misc_feature    1905..2402
                     /label=mutTAD_ABE8.17+V106W
     misc_feature    2403..2498
                     /label=32 aa linker
     CDS             2499..7382
                     /codon_start=1
                     /product="Cas9 endonuclease from the Francisella novicida 
                     Type II CRISPR/Cas system (Hirano et al., 2016)"
                     /label=nD11Aen31FnCas9
                     /translation="NFKILPIAIALGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKD
                     SYTLLMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFNRRGFS
                     FITDGYSPEYLNIVPEQVKAILMDIFDDYNGEDDLDSYLKLATEQESKISEIYNKLMQK
                     ILEFKLMKLCTDIKDDKVSTKTLKEITSYEFELLADYLANYSESLKTQKFSYTDKQGNL
                     KELSYYHHDKYNIQEFLKRHATINDRILDTLLTDDLDIWNFNFEKFDFDKNEEKLQNQE
                     DKDHIQAHLHHFVFAVNKIKSEMASGGRHRSQYFQEITNVLDENNHQEGYLKNFCENLH
                     NKKYSNLSVKNLVNLIGNLSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEW
                     RVGVKDQDKKDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPK
                     CQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKDQPYFVEYKS
                     SNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQAKKLKQKASSELEKLESS
                     KKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQRQRARDSRLYIMPEYRYDK
                     KLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQLLNDLAGVLQVSPNFLKDKIGSDDDLFI
                     SKWLVEHIRGFKKACEDSLKIQKDNRGLLNHKINIARNTKGKCEKEIFNLICKIEGSED
                     KKGNYKHGLAYELGVLLFGEPNEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANT
                     CAVCSADNAHRMQQIKITEPVEDNKDKIILSAKAQRLPAIPTRIVDGAVKKMATILAKN
                     IVDDNWQNIKQVLSAKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPEN
                     IFKDKNNRIKEFAKGISAYSGANLTDGDFDGAKEELDHIIPRSHKKYGTLNDEANLICV
                     TRGDNKNKGNRIFCLRDLADNYKLKQFETTDDLEIEKKIADTIWDANKKDFKFGNYRSF
                     INLTPQEQKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLRAK
                     KENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKPQASYSHLIDAM
                     LAFCIAADEHRNDGSIGLEIDKNYSLYPLDKNTGEVFTKDIFSQIKITDNEFSDKKLVR
                     KKAIEGFNTHRQMTRDTIYAENYLPILIHKELNEVRKGYTWKNSEEIKIFKGKKYDIQQ
                     LNNLVYCLKFVDKPISIDIQISTLEELRNILTTNNIAATAEYYYINLKTQKLHEYYIEN
                     YNTALGYKKYSKEMEFLRSLAYRSRRVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQ
                     RLYREWQNTTIKDDYEFLKSFFNVKSITKLHKKVRKDFSLPISTNHGKFLVKRKTWDNN
                     FIYQILNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKVDNKN
                     IFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVIDDDSKINYFMNHS
                     LLKSRYPDKVLEILKQSTIIEFESSGFNKTIKEMLGMKLAGIYNETSNN"
     misc_feature    6222..6224
                     /label=G1243T
     misc_feature    6600..6602
                     /label=E1369R
     misc_feature    6840..6842
                     /label=E1449H
     misc_feature    7383..7394
                     /label=Linker
     misc_feature    7395..7445
                     /label=bpNLS
     CDS             7425..7445
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7446..7457
                     /label=Linker
     CDS             7458..7538
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             7554..7607
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7608..8321
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             7608..8321
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    8355..8562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8571..8711
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      8786..9241
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9523..9627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9628..10488
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10659..11247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt tcagttgcgc cgaaaggcgc tctgtaatca
      301 tttaaaagta ttttgaacgg acctctgttt gacacgtctg ttttttgttt tagagctaga
      361 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
      421 tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      481 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
      661 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
      781 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgacgctgc
     1021 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     1081 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
     1141 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
     1201 gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg ggatccaaac
     1261 ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc tctgaagtcg
     1321 agtttagcca cgagtattgg atgaggcacg cactgaccct ggcaaagcga gcatgggatg
     1381 aaagagaagt ccccgtgggc gccgtgctgg tgcacaacaa tagagtgatc ggagagggat
     1441 ggaacaggcc aatcggccgc cacgacccta ccgcacacgc agagatcatg gcactgaggc
     1501 agggaggcct ggtcatgcag aattaccgcc tgatcgatgc caccctgtat gtgacactgg
     1561 agccatgcgt gatgtgcgca ggagcaatga tccacagcag gatcggaaga gtggtgttcg
     1621 gagcacggga cgccaagacc ggcgcagcag gctccctgat ggatgtgctg caccaccccg
     1681 gcatgaacca ccgggtggag atcacagagg gaatcctggc agacgagtgc gccgccctgc
     1741 tgagcgattt ctttagaatg cggagacagg agatcaaggc ccagaagaag gcacagagct
     1801 ccaccgactc tggaggatct agcggaggat cctctggaag cgagacacca ggcacaagcg
     1861 agtccgccac accagagagc tccggcggct cctccggagg atcctctgag gtggagtttt
     1921 cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc gatgagaggg
     1981 aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag ggctggaaca
     2041 gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg agacagggcg
     2101 gcctggtcat gcagaactac agactgattg acgccaccct gtacagcaca ttcgagcctt
     2161 gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg tttggctgga
     2221 ggaacgcaaa aaccggcgcc gcaggctccc tgatggacgt gctgcactac cccggcatga
     2281 atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc ctgctgtgct
     2341 atttctttcg gatgcctaga agggtgttca atgctcagaa gaaggcccag agctccaccg
     2401 actccggagg atctagcgga ggctcctctg gctctgagac acctggcaca agcgagagcg
     2461 caacacctga aagcagcggg ggcagcagcg gggggtcaaa ttttaagatc ctgcctatcg
     2521 caatcgccct gggcgtcaag aatactggcg tgtttagtgc tttttatcag aaggggacct
     2581 cactggagag actggacaat aagaacggaa aagtgtatga actgtccaag gattcttaca
     2641 ctctgctgat gaacaatagg accgcacgga gacaccagag gcgaggaatt gacaggaaac
     2701 agctggtgaa gcgcctgttc aaactgatct ggacagagca gctgaacctg gaatgggata
     2761 aggacactca gcaggccatc agcttcctgt ttaatcgacg gggattctct tttattactg
     2821 acggctatag tcctgagtac ctgaacatcg tgccagaaca ggtcaaggca atcctgatgg
     2881 acattttcga cgattataat ggcgaggacg atctggattc ctacctgaaa ctggccacag
     2941 agcaagagag taagatcagc gaaatctaca acaagctgat gcagaagatc ctggagttca
     3001 agctgatgaa actgtgcacc gacatcaagg acgataaagt gagtaccaag acactgaaag
     3061 agatcacaag ctacgagttc gaactgctgg ccgattatct ggctaactac agcgaatccc
     3121 tgaaaaccca gaaattttcc tacacagaca agcagggcaa tctgaaagag ctgtcttact
     3181 accaccatga taagtacaac atccaggagt tcctgaagag acacgccacc atcaatgaca
     3241 ggattctgga tacactgctg actgacgatc tggacatctg gaacttcaac ttcgagaagt
     3301 tcgatttcga caagaacgag gaaaaactgc agaatcagga agataaggac cacattcagg
     3361 ctcatctgca ccatttcgtg tttgcagtca ataagatcaa aagcgagatg gcatccggcg
     3421 ggcgccatcg aagccagtac ttccaggaaa tcaccaacgt gctggacgag aacaatcacc
     3481 aggaaggcta cctgaaaaac ttctgtgaga atctgcataa caagaagtac agcaatctgt
     3541 ccgtgaagaa tctggtcaac ctgattggaa atctgtccaa cctggaactg aagcccctgc
     3601 gcaaatactt caacgacaag atccacgcta aagcagacca ttgggatgag cagaagttta
     3661 ctgaaaccta ttgccactgg attctgggcg agtggcgggt gggggtcaag gatcaggaca
     3721 agaaagacgg cgcaaagtat tcttacaagg acctgtgtaa cgagctgaag cagaaagtga
     3781 ctaaggccgg gctggtggac ttcctgctgg agctggaccc ctgccgaacc attccacctt
     3841 acctggacaa caataacaga aagccaccca aatgtcagag cctgatcctg aatcccaagt
     3901 ttctggataa tcagtatcct aactggcagc agtacctgca ggagctgaag aaactgcagt
     3961 caatccagaa ctacctggac agcttcgaaa ccgatctgaa ggtgctgaaa agctccaagg
     4021 accagcctta cttcgtcgag tacaagtcta gtaaccagca gatcgcttcc ggccagcggg
     4081 attacaagga tctggacgca agaatcctgc agttcatttt tgacagggtg aaggcctctg
     4141 atgagctgct gctgaacgaa atctatttcc aggcaaagaa actgaagcag aaagcctcaa
     4201 gcgagctgga aaagctggag tcctctaaga aactggacga agtgatcgct aactctcagc
     4261 tgagtcagat tctgaagtct cagcacacaa atggaatctt cgagcagggc acttttctgc
     4321 atctggtgtg caaatactat aagcagcgac agagagccag ggacagccgc ctgtacatca
     4381 tgcctgaata tcgatacgat aagaaactgc acaagtacaa caacaccggc cgctttgacg
     4441 atgacaacca gctgctgaca tattgtaatc ataagccccg gcagaaaaga taccagctgc
     4501 tgaacgacct ggcaggagtg ctgcaggtct ctcctaattt tctgaaggat aaaatcgggt
     4561 ccgatgacga tctgttcatt tctaagtggc tggtggagca catccggggc tttaagaagg
     4621 cctgcgaaga tagcctgaaa atccagaagg ataacagggg actgctgaat cataagatca
     4681 acattgcacg caataccaag ggcaaatgcg agaaagaaat cttcaacctg atctgtaaga
     4741 ttgaggggag cgaagataag aaagggaatt ataagcacgg actggcctac gagctgggag
     4801 tgctgctgtt cggagagcca aacgaggcca gcaagcccga atttgatagg aaaatcaaga
     4861 aattcaattc aatctacagc tttgcccaga tccagcagat tgcctttgct gagaggaagg
     4921 ggaatgcaaa cacatgcgcc gtgtgtagtg cagacaacgc ccatcgcatg cagcagatca
     4981 aaattactga gccagtcgaa gataataagg ataaaatcat tctgtcagca aaggcacagc
     5041 gactgcctgc aatcccaacc cgaattgtgg atggagctgt caagaaaatg gctacaattc
     5101 tggcaaagaa tatcgtggac gataattggc agaacattaa gcaggtcctg agcgcaaaac
     5161 accagctgca tatcccaatc attaccgagt ccaacgcctt cgagtttgaa cccgctctgg
     5221 cagacgtgaa gggcaaatct ctgaaggata gaaggaagaa agccctggag cgaattagtc
     5281 ccgaaaacat cttcaaggat aagaacaaca gaatcaagga gtttgctaag gggatttccg
     5341 cctactctgg agctaacctg acagatgggg acttcgatgg agcaaaggag gaactggatc
     5401 acatcattcc tcgcagccat aagaaatatg gcactctgaa cgacgaggct aatctgattt
     5461 gcgtgacccg gggcgataat aagaacaaag ggaaccggat cttctgtctg agagacctgg
     5521 ccgataatta caagctgaaa cagtttgaga ccacagacga tctggagatc gaaaagaaaa
     5581 ttgccgacac catctgggat gctaataaga aggacttcaa gttcggaaac tatcggagct
     5641 tcatcaatct gacacctcag gagcagaaag cattcagaca cgccctgttt ctggctgatg
     5701 aaaacccaat caagcaggca gtgatcagag ccattaataa ccgcaaccga accttcgtga
     5761 atggcacaca gaggtatttt gctgaggtcc tggcaaataa catctacctg cgcgccaaga
     5821 aagaaaatct gaacactgac aagatcagct tcgattactt tggaatccct accattggaa
     5881 acggccgagg gatcgctgag attcggcagc tgtatgaaaa ggtggacagt gatatccagg
     5941 cctacgctaa aggcgacaag ccacaggcct cttatagtca cctgattgat gctatgctgg
     6001 cattctgcat cgccgctgac gagcatcgga acgatggatc tattggcctg gaaatcgaca
     6061 aaaactatag tctgtaccct ctggataaga atactggcga ggtgttcacc aaagacatct
     6121 tttcacagat caagattacc gacaacgagt tcagcgataa gaaactggtc agaaagaaag
     6181 ctattgaagg gtttaacaca cacagacaga tgactaggga taccatctat gcagagaatt
     6241 acctgcctat cctgattcat aaggagctga acgaagtgag gaaggggtac acatggaaaa
     6301 attccgagga aatcaaaatt ttcaagggaa agaaatacga catccagcag ctgaataacc
     6361 tggtgtattg tctgaagttt gtggacaaac caatcagtat tgatatccag atttcaaccc
     6421 tggaggaact gagaaacatc ctgactacca ataacattgc agccactgcc gagtactatt
     6481 acattaatct gaaaacccag aagctgcacg agtattacat cgaaaattac aacacagccc
     6541 tggggtataa gaaatacagc aaggagatgg agttcctgag gtccctggct tataggtcta
     6601 ggcgcgtgaa gatcaaaagt attgacgatg tcaagcaggt cctggacaag gattcaaact
     6661 tcatcatcgg aaagatcaca ctgcccttca agaaagagtg gcagcgactg taccgggaat
     6721 ggcagaacac aactatcaaa gacgattatg agtttctgaa gagcttcttt aatgtgaagt
     6781 ccattactaa actgcacaag aaagtccgga aagacttctc tctgcccatc agtacaaacc
     6841 acggcaagtt tctggtgaag agaaaaactt gggataataa cttcatctac cagattctga
     6901 atgactcaga tagcagggca gacgggacta aacccttcat tcctgccttt gatatcagca
     6961 agaacgagat tgtggaagcc atcattgaca gtttcacctc aaaaaacatc ttttggctgc
     7021 caaagaatat tgagctgcag aaggtggaca acaagaacat cttcgccatt gataccagca
     7081 agtggtttga ggtcgaaaca ccatccgacc tgcgcgatat cggcattgct accattcagt
     7141 acaagatcga caataactca cgccccaagg tgcgagtcaa actggattac gtgatcgacg
     7201 atgacagcaa gattaactat ttcatgaatc actcactgct gaagagccgg tatcccgaca
     7261 aagtcctgga gatcctgaag cagagcacaa tcattgagtt cgaaagttca gggtttaaca
     7321 aaactattaa ggagatgctg ggaatgaagc tggccggcat ctacaatgaa acctccaata
     7381 actctggcgg ctcaaaaaga accgccgacg gcagcgagtt cgagcccaag aagaagagga
     7441 aagtcagcgg cggcagctac ccatacgatg ttccagatta cgcttaccca tacgatgttc
     7501 cagattacgc ttacccatac gatgttccag attacgctga attcggcagt ggagagggca
     7561 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccagtg agcaagggcg
     7621 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     7681 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     7741 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     7801 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     7861 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     7921 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     7981 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     8041 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     8101 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     8161 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     8221 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     8281 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggagttctaa ctagagctcg
     8341 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8401 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8461 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8521 caagggggag gattgggaag agaatagcag gcatgctggg gagcggccgc aggaacccct
     8581 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     8641 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     8701 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8761 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8821 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8881 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8941 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9001 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9061 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9121 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9181 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     9241 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     9301 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     9361 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     9421 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9481 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9541 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9601 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9661 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9721 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9781 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9841 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9901 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9961 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10021 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10081 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10141 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10201 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10261 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10321 aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt
    10381 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10441 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10501 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10561 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10621 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10681 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10741 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10801 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10861 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10921 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10981 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11041 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11101 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11161 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11221 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    11281 gccttttgct ggccttttgc tcacatgt
//
