LOCUS       pMC-J6-NbGP41-sfGFP-VP6 3871 bp ds-DNA     circular     24-APR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      35..717
                     /label="ColE1 origin"
     misc_feature    100..714
                     /label="rep\(pMB1)"
     rep_origin      complement(699..699)
                     /label="ORI"
     misc_feature    947..950
                     /label="J6/AATG"
     misc_feature    948..1316
                     /label="GP41(2H10)"
     CDS             948..2570
                     /label="Translation 948-2570"
                     /translation="MEVQLVESGGGLVQPGGSLRLSCAASGSISSVDVMSWYRQAPGKQRELVAFITDRGRTNYKVSVKGRFTISRDNSKNMVYLQMNSLKPEDTADYLCRAESRTSWSSPSPLDVWGRGTQVTVSSLDPGGGGSGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGGGRTGRTGGGGGDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSGGGGGAPKKKRKVRTGGGSRTEEYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEGGGSGGGTSPKTRRRPRRSQRKRPPTPWP*"
     misc_feature    1344..2054
                     /label="sfGFP"
     CDS             2094..2243
                     /label="VP64"
     CDS             2268..2288
                     /label="SV40 NLS"
     CDS             2319..2480
                     /label="GB1"
     CDS             2508..2558
                     /label="Rex NLS"
     misc_feature    2571..2574
                     /label="J9/GCTT"
     CDS             2801..3811
                     /label="Sm/Sp\no\DraIII"
     mutation        3778..3778
                     /label="G\to\A"
ORIGIN
        1 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
       61 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      241 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
      301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      901 cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaAatg gaggtgcagc
      961 tggtggaatc tgggggcgga ctggtgcagc ccgggggatc tctgcggctg tcctgcgccg
     1021 cctctggctc catctctagc gtggatgtga tgtcctggta caggcaggcc cctggcaagc
     1081 agagggaact ggtggccttc attactgata ggggaagaac caattacaaa gtgagcgtga
     1141 aaggccgctt cactatcagc cgggataatt ctaagaatat ggtgtatctg cagatgaaca
     1201 gcctgaagcc agaagacact gccgactatc tgtgcagggc tgagtcccgg acttcctggt
     1261 ccagcccatc tcctctggat gtgtgggggc gcggcactca ggtgactgtg tcctccttgg
     1321 atccaggtgg aggtggaagc ggtagcaaag gagaagaact tttcactgga gttgtcccaa
     1381 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg
     1441 aaggtgatgc tacaaacgga aaactcaccc ttaaatttat ttgcactact ggaaaactac
     1501 ctgttccgtg gccaacactt gtcactactc tgacctatgg tgttcaatgc ttttcccgtt
     1561 atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
     1621 aggaacgcac tatatctttc aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt
     1681 ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt aaagaagatg
     1741 gaaacattct tggacacaaa ctcgagtaca actttaactc acacaatgta tacatcacgg
     1801 cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac gttgaagatg
     1861 gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     1921 ttttaccaga caaccattac ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa
     1981 agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg
     2041 atgagctcta caaaggtgga ggtcggaccg gtcgtacggg cggtggcgga ggggatgctt
     2101 tagacgattt tgacttagat atgcttggtt cagacgcgtt agacgacttc gacctagaca
     2161 tgttaggctc agatgcattg gacgacttcg atttagatat gttgggctcc gatgccctag
     2221 atgactttga tctagatatg ctaggtagtg gcggtggcgg aggggctcca aagaagaaga
     2281 gaaaggtccg tacgggagga ggatctcgga ccgaagagta caagcttatc ctgaacggta
     2341 aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt
     2401 tcaaacagta cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca
     2461 aaaccttcac ggtaaccgaa ggtggtggta gcggtggtgg tactagtcca aaaacaagga
     2521 ggagGccgcg aagatcacaa cggaaaaggc cgcctacgcc atggccgtaa Gctttgagac
     2581 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2641 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     2821 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2881 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2941 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3001 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3061 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3121 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     3181 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3241 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3301 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3361 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3421 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3481 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3541 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3601 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3661 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     3721 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa
     3781 aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact catatatact
     3841 ttagattgat ttaaaacttc atttttaatt t
//