LOCUS       1BR9-AtWUS_stop         6399 bp ds-DNA     circular     05-MAY-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 1B.ape >1B Ecoli T7
            ColE1-ori LICv1 His6-tev-yORF KAN ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(37..146)
                     /label="RNA1-for"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(585..594)
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1018..1243)
                     /label="ROP/ROM"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(2006..2026)
                     /label="Lac O3"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     misc_feature    complement(2019..3101)
                     /label="lac repressor"
                     /ApEinfo_revcolor="#ff2344"
                     /ApEinfo_fwdcolor="#ff2344"
     misc_feature    complement(3102..3179)
                     /label="lacI wt pro"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     3488..3507
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    3507..3529
                     /label="Lac O1"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     CDS             3575..4603
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGSSHHHHHHENLYFQSNARDHMVLHEYVNAAGITGGGGSGGGGSYFQSNAEPPQHQHHHHQADQESGNNNNNKSGSGGYTCRQTSTRWTPTTEQIKILKELYYNNAIRSPTADQIQKITARLRQFGKIEGKNVFYWFQNHKARERQKKRFNGTNMTTPSSSPNSVMMAANDHYHPLLHHHHGVPMQRPANSVNVKLNQDHHLYHHNKPYPSFNNGNLNHASSGTECGVVNASNGYMSSHVYGSMEQDCSMNYNNVGGGWANMDHHYSSAPYNFFDRAKPLFGLEGHQEEEECGGDAYLEHRRTLPLFPMHGEDHINGGSGAIWKYGQSEVRPCASLELRLN*"
     misc_feature    complement(3948..3967)
                     /label="wusp excision rv"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3968..4030
                     /label="WUSP"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4031..4051
                     /label="wusp exp fw"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(4680..4684)
                     /label="v1 RV"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4685..4699
                     /label="Lic_v1_rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4817..4835
                     /label="T7 terminator primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4838..4878
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4932..5238
                     /label="F1 ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(5463..6278)
                     /label="Kan R phosphotransferase"
                     /ApEinfo_revcolor="#7261ff"
                     /ApEinfo_fwdcolor="#7261ff"
     rep_origin      6324..607
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       61 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
      121 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
      181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
      241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
      301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
      361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
      421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
      481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
      541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
      601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
      661 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
      721 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT
      781 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC
      841 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG
      901 ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT
      961 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC
     1021 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT
     1081 GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT
     1141 CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT
     1201 GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA
     1261 TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG
     1321 AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC
     1381 AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC
     1441 ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC
     1501 TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC
     1561 AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC
     1621 AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGGG
     1681 CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA
     1741 CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG
     1801 TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC
     1861 CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG
     1921 CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG
     1981 CCTAATGAGT GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG
     2041 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC
     2101 GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC
     2161 TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG
     2221 CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG
     2281 TCGTATCCCA CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC
     2341 ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA
     2401 TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC
     2461 GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC
     2521 GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC
     2581 AGATGCTCCA CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT
     2641 GTCTGGTCAG AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA
     2701 ATGGCATCCT GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA
     2761 AGATTGTGCA CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC
     2821 ACGCTGGCAC CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG
     2881 TGCAGGGCCA GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT
     2941 TGTGCCACGC GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC TTTTTCCCGC
     3001 GTTTTCGCAG AAACGTGGCT GGCCTGGTTC ACCACGCGGG AAACGGTCTG ATAAGAGACA
     3061 CCGGCATACT CTGCGACATC GTATAACGTT ACTGGTTTCA CATTCACCAC CCTGAATTGA
     3121 CTCTCTTCCG GGCGCTATCA TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTCC
     3181 GGGATCTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT
     3241 GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG
     3301 TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA
     3361 GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC
     3421 TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC
     3481 GCGAAATTAA TACGACTCAC TATAGGGGAA TTGTGAGCGG ATAACAATTC CCCTCTAGAA
     3541 ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCATGGGT TCTTCTCACC ATCACCATCA
     3601 CCATGAAAAC CTGTACTTCC AATCCAATgc acgcgatcac atggtcctgc acgagtacgt
     3661 gaacgccgcc gggatcactG GTGGCGGAGG TTCTGGAGGC GGTGGATCGt acttccaatc
     3721 caatgcaGAA CCTCCCCAGC ACCAGCACCA TCACCACCAG GCTGATCAAG AGTCGGGAAA
     3781 TAACAACAAC AATAAAAGCG GGAGCGGGGG TTACACCTGT CGTCAAACAT CCACGCGCTG
     3841 GACACCAACC ACTGAACAAA TCAAAATTTT GAAAGAATTA TACTATAACA ATGCCATTCG
     3901 TTCACCTACC GCAGATCAAA TCCAAAAAAT TACAGCACGC TTGCGCCAGT TCGGAAAGAT
     3961 TGAGGGAAAA AACGTGTTCT ACTGGTTTCA GAATCATAAG GCACGTGAAC GTCAAAAAAA
     4021 ACGTTTTAAC GGGACTAATA TGACAACACC GAGTTCGAGT CCCAACTCCG TTATGATGGC
     4081 AGCGAACGAT CACTATCATC CATTACTGCA CCACCATCAT GGAGTCCCAA TGCAGCGTCC
     4141 AGCCAATTCC GTGAATGTCA AGCTGAACCA AGACCACCAT CTTTATCACC ACAATAAGCC
     4201 TTACCCATCT TTTAATAACG GCAACCTGAA TCACGCCAGC AGTGGCACCG AATGCGGGGT
     4261 CGTCAATGCG TCGAATGGAT ATATGTCGTC GCACGTCTAC GGTTCTATGG AACAAGACTG
     4321 TTCAATGAAT TACAACAACG TAGGAGGGGG CTGGGCCAAT ATGGACCATC ACTACAGCTC
     4381 TGCGCCGTAC AATTTCTTCG ACCGTGCTAA ACCGTTATTC GGCTTAGAGG GACATCAGGA
     4441 GGAGGAGGAA TGCGGGGGTG ACGCTTACTT AGAACATCGT CGTACTTTAC CGCTTTTCCC
     4501 GATGCATGGA GAGGACCATA TCAATGGTGG GTCTGGCGCC ATTTGGAAGT ACGGACAGTC
     4561 GGAGGTACGC CCTTGCGCAA GTCTTGAGCT GCGTTTgaat tagtaataac ATTGGTAGTG
     4621 GGAGCAACGG CAGCAGCGGA TCCGTGAGCC GCCGTCGCCG TCGCCGTCGC CGTCGCTAAT
     4681 AACAATTGGA AGTGGATAAC GGATCCGAAT TCGAGCGCCG TCGACAAGCT TGCGGCCGCA
     4741 CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT
     4801 GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     4861 GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATTGGCGAA TGGGACGCGC
     4921 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC
     4981 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG
     5041 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT
     5101 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC
     5161 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT
     5221 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA
     5281 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA
     5341 ATTTTAACAA ACTAGTAACG TTTACAATTT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     5401 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAATTAAT
     5461 TCTTAGAAAA ACTCATCGAG CATCAAATGA AACTGCAATT TATTCATATC AGGATTATCA
     5521 ATACCATATT TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCAGTTC
     5581 CATAGGATGG CAAGATCCTG GTATCGGTCT GCGATTCCGA CTCGTCCAAC ATCAATACAA
     5641 CCTATTAATT TCCCCTCGTC AAAAATAAGG TTATCAAGTG AGAAATCACC ATGAGTGACG
     5701 ACTGAATCCG GTGAGAATGG CAAAAGTTTA TGCATTTCTT TCCAGACTTG TTCAACAGGC
     5761 CAGCCATTAC GCTCGTCATC AAAATCACTC GCATCAACCA AACCGTTATT CATTCGTGAT
     5821 TGCGCCTGAG CGAGACGAAA TACGCGATCG CTGTTAAAAG GACAATTACA AACAGGAATC
     5881 GAATGCAACC GGCGCAGGAA CACTGCCAGC GCATCAACAA TGTTTTCACC TGAATCAGGA
     5941 TATTCTTCTA ATACCTGGAA TGCTGTTTTC CCGGGGATCG CAGTGGTGAG TAACCATGCA
     6001 TCATCAGGAG TACGGATAAA ATGCTTGATG GTCGGAAGAG GCATAAATTC CGTCAGCCAG
     6061 TTTAGTCTGA CCATCTCATC TGTAACATCA TTGGCAACGC TACCTTTGCC ATGTTTCAGA
     6121 AACAACTCTG GCGCATCGGG CTTCCCATAC AATCGATAGA TTGTCGCACC TGATTGCCCG
     6181 ACATTATCGC GAGCCCATTT ATACCCATAT AAATCAGCAT CCATGTTGGA ATTTAATCGC
     6241 GGCCTAGAGC AAGACGTTTC CCGTTGAATA TGGCTCATAA CACCCCTTGT ATTACTGTTT
     6301 ATGTAAGCAG ACAGTTTTAT TGTTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
     6361 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTC
//