LOCUS       pMC_N_donor2            5559 bp    DNA        circular     25-OCT-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    782..816
                     /locus_tag="attB"
                     /label="attB"
                     /ApEinfo_label="attB"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1..738
                     /locus_tag="32 consecutive ISce1 site"
                     /label="32 consecutive ISce1 site"
                     /ApEinfo_label="32 consecutive ISce1 site"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2425..3219)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3522..5559
                     /locus_tag="EcoE1 origin"
                     /label="EcoE1 origin"
                     /ApEinfo_label="EcoE1 origin"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1678..1680
                     /locus_tag="PAM"
                     /label="PAM"
                     /ApEinfo_label="PAM"
                     /ApEinfo_fwdcolor="#f647a6"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    816..820
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    1658..1677
                     /locus_tag="Frame Selector +1 target"
                     /label="Frame Selector +1 target"
                     /ApEinfo_label="Frame Selector +1 target"
                     /ApEinfo_fwdcolor="#fc700d"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2293..2293
                     /locus_tag="MCS(1)"
                     /label="MCS(1)"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2300..2338
                     /locus_tag="attP"
                     /label="attP"
                     /ApEinfo_label="attP"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2278..2287
                     /locus_tag="Donor site"
                     /label="Donor site"
                     /ApEinfo_label="Donor site"
                     /ApEinfo_fwdcolor="#63c6ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             868..921
                     /codon_start="1"
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    853..855
                     /locus_tag="Acceptor site"
                     /label="Acceptor site"
                     /ApEinfo_label="Acceptor site"
                     /ApEinfo_fwdcolor="#8ac8ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    922..1629
                     /locus_tag="mNG gblock"
                     /label="mNG gblock"
                     /ApEinfo_label="mNG gblock"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    830..834
                     /locus_tag="Branch point"
                     /label="Branch point"
                     /ApEinfo_label="Branch point"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             922..1629
                     /locus_tag="codon optimazed mNG"
                     /label="codon optimazed mNG"
                     /ApEinfo_label="codon optimazed mNG"
                     /ApEinfo_fwdcolor="#00a43a"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    835..851
                     /locus_tag="Polypyrimiding region"
                     /label="Polypyrimiding region"
                     /ApEinfo_label="Polypyrimiding region"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2278..2283
                     /locus_tag="Conserved donor site"
                     /label="Conserved donor site"
                     /ApEinfo_label="Conserved donor site"
                     /ApEinfo_fwdcolor="#4fa1ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1681..2277
                     /locus_tag="PuroR"
                     /label="PuroR"
                     /ApEinfo_label="PuroR"
                     /ApEinfo_fwdcolor="#00e8e3"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1657..1676
                     /locus_tag="Frame Selector +2 target"
                     /label="Frame Selector +2 target"
                     /ApEinfo_label="Frame Selector +2 target"
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1659..1677
                     /locus_tag="Frame selector +0 target"
                     /label="Frame selector +0 target"
                     /ApEinfo_label="Frame selector +0 target"
                     /ApEinfo_fwdcolor="#f99400"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1408..1429
                     /locus_tag="41 mNG 3 primer"
                     /label="41 mNG 3 primer"
                     /ApEinfo_label="41 mNG 3 primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2078..2097
                     /locus_tag="128 puroF"
                     /label="128 puroF"
                     /ApEinfo_label="128 puroF"
                     /ApEinfo_fwdcolor="#ebfd00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ACATTACCCT GTTATCCCTA GATACATTAC CCTGTTATCC CAGATGACAT ACCCTGTTAT
       61 CCCTAGATGA CATTACCCTG TTATCCCAGA TGACATTACC CTGTTATCCC TAGATACATT
      121 ACCCTGTTAT CCCAGATGAC ATACCCTGTT ATCCCTAGAT GACATTACCC TGTTATCCCA
      181 GATGACATTA CCCTGTTATC CCTAGATACA TTACCCTGTT ATCCCAGATG ACATACCCTG
      241 TTATCCCTAG ATGACATTAC CCTGTTATCC CAGATGACAT TACCCTGTTA TCCCTAGATA
      301 CATTACCCTG TTATCCCAGA TGACATACCC TGTTATCCCT AGATGACATT ACCCTGTTAT
      361 CCCAGATGAC ATTACCCTGT TATCCCTAGA TACATTACCC TGTTATCCCA GATGACATAC
      421 CCTGTTATCC CTAGATGACA TTACCCTGTT ATCCCAGATG ACATTACCCT GTTATCCCTA
      481 GATACATTAC CCTGTTATCC CAGATGACAT ACCCTGTTAT CCCTAGATGA CATTACCCTG
      541 TTATCCCAGA TGACATTACC CTGTTATCCC TAGATACATT ACCCTGTTAT CCCAGATGAC
      601 ATACCCTGTT ATCCCTAGAT GACATTACCC TGTTATCCCA GATGACATTA CCCTGTTATC
      661 CCTAGATACA TTACCCTGTT ATCCCAGATG ACATACCCTG TTATCCCTAG ATGACATTAC
      721 CCTGTTATCC CAGATAAACT CAATGATGAT GATGATGATG GTCGAGACTC AGCGGCCGCG
      781 GTGCCAGGGC GTGCCCTTGG GCTCCCCGGG CGCGACTAGT GTGACGCTGC TGACCCCTTT
      841 CTTTCCCTTC TGCAGggttc cggaggagag ggcagaggaa gtctgctaac atgcggtgac
      901 gtcgaggaga atcctggccc aATGGTTAGT AAAGGTGAGG AGGACAACAT GGCAAGTCTC
      961 CCCGCTACTC ACGAACTTCA TATCTTCGGC TCCATAAACG GGGTTGACTT TGATATGGTA
     1021 GGGCAGGGAA CAGGGAATCC TAATGACGGC TATGAAGAGC TCAATTTGAA GTCAACCAAG
     1081 GGTGATCTCC AGTTCTCACC CTGGATTCTG GTCCCACATA TAGGTTACGG ATTCCACCAG
     1141 TACCTTCCTT ACCCTGATGG GATGAGTCCA TTTCAAGCTG CCATGGTAGA TGGTAGCGGG
     1201 TACCAGGTCC ATCGCACTAT GCAGTTTGAA GACGGTGCTT CATTGACTGT TAATTACCGC
     1261 TACACATACG AAGGCTCTCA CATTAAAGGC GAAGCCCAGG TAAAAGGCAC AGGATTTCCC
     1321 GCTGACGGAC CTGTTATGAC AAATTCCCTG ACCGCAGCAG ACTGGTGCAG GAGTAAAAAG
     1381 ACTTATCCCA ATGATAAGAC AATTATCAGT ACATTCAAGT GGAGCTATAC TACCGGCAAC
     1441 GGCAAAAGAT ATCGAAGTAC AGCCCGGACA ACTTATACAT TTGCTAAACC AATGGCCGCT
     1501 AATTATCTCA AAAATCAACC CATGTACGTC TTCAGAAAAA CAGAGCTGAA ACACAGTAAG
     1561 ACAGAGCTTA ATTTTAAAGA ATGGCAAAAG GCCTTCACCG ATGTTATGGG CATGGATGAG
     1621 CTCTACAAAg ggtctggtgg cagtggaggg ggatccgggc cagtacccaa aaagcggGGG
     1681 atgactgaat acaagcctac tgtcaggttg gctacaagag acgacgttcc tagagccgtg
     1741 agaactctgg ctgcagcctt cgccgactac cccgccacga gacacaccgt tgacccagat
     1801 cggcatattg agagagtgac tgaactgcag gagctgtttc ttacaagagt tggcctcgac
     1861 ataggcaagg tgtgggtggc ggacgacggc gccgccgtgg ccgtctggac cactcccgaa
     1921 tcagttgagg ctggcgccgt attcgctgag atcggcccga gaatggctga gctcagcggg
     1981 agtaggctcg cggcacagca gcaaatggag ggactgctgg caccacacag gcccaaagaa
     2041 cccgcctggt tcctggcaac cgtcggtgta tctcccgatc atcaggggaa aggtctgggc
     2101 tctgccgtag tgctccctgg cgtggaggca gctgagagag caggagtacc tgccttcttg
     2161 gagacctccg ctccaaggaa tcttcccttc tatgaacggt tgggcttcac cgtgacagcc
     2221 gacgtggaag tccccgaagg cccccgcact tggtgcatga cgaggaagcc tggagcgGTA
     2281 AGTCGACTCG TTGGGCCCGC CCCAACTGGG GTAACCTTTG AGTTCTCTCA GTTGGGGGTA
     2341 ATCAGCATCA TGATGTGGTA CCACATCATG ATGCTGATTA TAAGAATGCG GCCGCCACAC
     2401 TCTAGTGGAT CTCGAGTTAA TAATTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT
     2461 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC
     2521 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC
     2581 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG
     2641 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG
     2701 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC
     2761 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC
     2821 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA
     2881 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA
     2941 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC
     3001 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA
     3061 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC
     3121 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC
     3181 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT
     3241 CTCTTGATCA GAGCTTGATC CCCTGCGCCA TCAGATCCTT GGCGGCGAGA AAGCCATCCA
     3301 GTTTACTTTG CAGGGCTTCC CAACCTTACC AGAGGGCGCC CCAGCTGGCA ATTCCGGTTC
     3361 GCTTGCTGTC CATAAAACCG CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC
     3421 CTGCTTTCTC TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT
     3481 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTG CTCGAGgggG gccAAACGGT
     3541 CTCCAGCTTG GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA GATTAAATCA
     3601 GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC GGTGGTCCCA
     3661 CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG TGTGGGGTCT
     3721 CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA
     3781 CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC
     3841 GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC
     3901 GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC
     3961 GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
     4021 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     4081 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     4141 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     4201 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     4261 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     4321 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     4381 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     4441 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     4501 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     4561 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     4621 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     4681 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     4741 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     4801 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     4861 AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT
     4921 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC
     4981 GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG
     5041 ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG
     5101 CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCAGA TCAATTCGCG
     5161 CGCGAAGGCG AAGCGGCATG CATAATGTGC CTGTCAAATG GACGAAGCAG GGATTCTGCA
     5221 AACCCTATGC TACTCCGTCA AGCCGTCAAT TGTCTGATTC GTTACCAATT ATGACAACTT
     5281 GACGGCTACA TCATTCACTT TTTCTTCACA ACCGGCACGG AACTCGCTCG GGCTGGCCCC
     5341 GGTGCATTTT TTAAATACCC GCGAGAAATA GAGTTGATCG TCAAAACCAA CATTGCGACC
     5401 GACGGTGGCG ATAGGCATCC GGGTGGTGCT CAAAAGCAGC TTCGCCTGGC TGATACGTTG
     5461 GTCCTCGCGC CAGCTTAAGA CGCTAATCCC TAACTGCTGG CGGAAAAGAT GTGACAGACG
     5521 CGACGGCGAC AAGCAAACAT GCTGTGCGAC GCTGGCGAT  
//
