LOCUS       Exported                2776 bp ds-DNA     circular UNA 25-FEB-2018
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     complement(49..72)
                     /label=seq 4 R
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /label=pMB1-pUC19 ORI variant
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_feature    502
                     /locus_tag="pMB1 high-copy number mutation"
                     /label=pMB1 high-copy number mutation
     misc_feature    complement(740..1174)
                     /locus_tag="SpecR"
                     /label=SpecR
     misc_feature    complement(740..1174)
                     /locus_tag="Spec gene(1)"
                     /label=Spec gene
                     /label=Spec gene(1)
     misc_feature    complement(740..1174)
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label=pro-CDS-Ter of SpecR
                     /label=pro-CDS-Ter of SpecR(1)
     misc_feature    complement(1172..1174)
                     /locus_tag="BspMI(1)"
                     /label=BspMI
                     /label=BspMI(1)
     misc_feature    complement(1172..1174)
                     /locus_tag="BspMI(4)"
                     /label=BspMI
                     /label=BspMI(4)
     misc_feature    complement(1176..1712)
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /label=pro-CDS-Ter of SpecR
     misc_feature    complement(1176..1531)
                     /locus_tag="Spec gene"
                     /label=Spec gene
     misc_feature    complement(1176..1531)
                     /locus_tag="SpecR(1)"
                     /label=SpecR
                     /label=SpecR(1)
     misc_feature    complement(1176..1177)
                     /locus_tag="BspMI"
                     /label=BspMI
     misc_feature    complement(1176..1177)
                     /locus_tag="BspMI(5)"
                     /label=BspMI
                     /label=BspMI(5)
     misc_feature    1190..1208
                     /locus_tag="SpecR R"
                     /label=SpecR R
     misc_feature    complement(1207..1226)
                     /locus_tag="SpecR F"
                     /label=SpecR F
     misc_feature    1292
                     /locus_tag="Mut G->A, silent"
                     /label=Mut G->A, silent
     misc_feature    complement(1297..1302)
                     /locus_tag="BpmI"
                     /label=BpmI
     promoter        complement(1532..1679)
                     /locus_tag="specR_promoter_from_pKD13"
                     /label=specR_promoter_from_pKD13
     misc_feature    1566
                     /locus_tag="Mut C->A"
                     /label=Mut C->A
     misc_feature    complement(1694..1696)
                     /locus_tag="BspMI(3)"
                     /label=BspMI
                     /label=BspMI(3)
     misc_feature    complement(1698..1699)
                     /locus_tag="BspMI(2)"
                     /label=BspMI
                     /label=BspMI(2)
     primer_bind     1833..1857
                     /label=seq 1 F
     misc_feature    complement(1885..1926)
                     /locus_tag="In T-DNA region AH003392.2"
                     /label=In T-DNA region AH003392.2
     primer_bind     1894..1916
                     /label=seq 2 F
     misc_feature    1927..1966
                     /locus_tag="UNS1"
                     /label=UNS1
     primer_bind     1934..1957
                     /label=Rev Loop Col PCR F
     primer_bind     1959..1997
                     /label=Loop 420
     misc_feature    1968..1973
                     /label=BsaI_F
                     /label=nonstandard type:  BsaI_F
     misc_feature    1975..1978
                     /label=Overhang
                     /label=CD OH
                     /label=nonstandard type:  Overhang
     gene            1979..2692
                     /label=roGFP
     primer_bind     complement(2669..2712)
                     /label=Loop 421
     misc_feature    2693..2694
                     /label=serine linker
                     /note="/feature_type=Linker region"
     misc_feature    complement(2695..2698)
                     /label=Overhang
                     /label=Overhang(1)
                     /label=CD OH
                     /label=nonstandard type:  Overhang
     misc_feature    complement(2700..2705)
                     /label=BsaI_R
                     /label=nonstandard type:  BsaI_R
     misc_feature    2707..2746
                     /locus_tag="UNSX"
                     /label=UNSX
     primer_bind     complement(2727..2746)
                     /label=Rev Loop Col PCR R
     misc_feature    2747..2776
                     /locus_tag="Extra region of pBR322"
                     /label=Extra region of pBR322
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgcattct aggtgattat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      781 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      841 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      901 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      961 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1021 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1081 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1141 gatcaatgtc gatcgtggct ggctcgaaga taccagcaag aatgtcattg cgctgccatt
     1201 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1261 caatggtgac ttctacagcg cggagaatct cactctctcc aggggaagcc gaagtttcca
     1321 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1381 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1441 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1501 tcgatacttc ggcgatcacc gcttccctca tgcgaaacga tcctcatcct gtctcttgat
     1561 cagatattga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     1621 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgcta
     1681 agcttgcatg cctgcaagtc gactctagag gagcggtgat cacaggcagc aacgctctgt
     1741 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     1801 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     1861 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     1921 cgagctcatt actcgcatcc attctcaggc tgtctcgtct cgtctcaggt ctcaaatggt
     1981 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2041 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2101 gctgaccctg aagttcatct ccaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2161 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2221 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2281 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2341 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2401 ggagtacaac tacaactgcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2461 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     2521 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2581 gagcacctgc tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2641 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtcaggtcg
     2701 agaccaccag gatacataga ttaccacaac tccgagccct tccacccaca gaatcagggg
     2761 ataacgcagg aaagaa
//
