LOCUS       Exported                2774 bp ds-DNA     circular UNA 01-MAR-2018
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /label=pMB1-pUC19 ORI variant
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     misc_feature    502
                     /locus_tag="pMB1 high-copy number mutation"
                     /label=pMB1 high-copy number mutation
     misc_feature    complement(740..1174)
                     /locus_tag="SpecR"
                     /label=SpecR
     misc_feature    complement(740..1174)
                     /locus_tag="Spec gene(1)"
                     /label=Spec gene
                     /label=Spec gene(1)
     misc_feature    complement(740..1174)
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label=pro-CDS-Ter of SpecR
                     /label=pro-CDS-Ter of SpecR(1)
     misc_feature    complement(1172..1174)
                     /locus_tag="BspMI(1)"
                     /label=BspMI
                     /label=BspMI(1)
     misc_feature    complement(1172..1174)
                     /locus_tag="BspMI(4)"
                     /label=BspMI
                     /label=BspMI(4)
     misc_feature    complement(1176..1712)
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /label=pro-CDS-Ter of SpecR
     misc_feature    complement(1176..1531)
                     /locus_tag="Spec gene"
                     /label=Spec gene
     misc_feature    complement(1176..1531)
                     /locus_tag="SpecR(1)"
                     /label=SpecR
                     /label=SpecR(1)
     misc_feature    complement(1176..1177)
                     /locus_tag="BspMI"
                     /label=BspMI
     misc_feature    complement(1176..1177)
                     /locus_tag="BspMI(5)"
                     /label=BspMI
                     /label=BspMI(5)
     misc_feature    1190..1208
                     /locus_tag="SpecR R"
                     /label=SpecR R
     misc_feature    complement(1207..1226)
                     /locus_tag="SpecR F"
                     /label=SpecR F
     misc_feature    1292
                     /locus_tag="Mut G->A, silent"
                     /label=Mut G->A, silent
     misc_feature    complement(1297..1302)
                     /locus_tag="BpmI"
                     /label=BpmI
     promoter        complement(1532..1679)
                     /locus_tag="specR_promoter_from_pKD13"
                     /label=specR_promoter_from_pKD13
     misc_feature    1566
                     /locus_tag="Mut C->A"
                     /label=Mut C->A
     misc_feature    complement(1694..1696)
                     /locus_tag="BspMI(3)"
                     /label=BspMI
                     /label=BspMI(3)
     misc_feature    complement(1698..1699)
                     /locus_tag="BspMI(2)"
                     /label=BspMI
                     /label=BspMI(2)
     misc_feature    complement(1885..1926)
                     /locus_tag="In T-DNA region AH003392.2"
                     /label=In T-DNA region AH003392.2
     primer_bind     1899..1926
                     /label=Loop colony PCR F
     misc_feature    1927..1966
                     /locus_tag="UNS1"
                     /label=UNS1
     primer_bind     1953..2048
                     /label=Loop 208
     misc_feature    1968..1973
                     /label=BsaI
     misc_feature    1975..1978
                     /locus_tag="D"
                     /label=D
     CDS             1979..2692
                     /label=sfGFP
     primer_bind     complement(2666..2722)
                     /label=Loop 209
     primer_bind     2693..2730
                     /label=L0 BB E OH-F
     misc_feature    complement(2693..2696)
                     /locus_tag="E"
                     /label=E
     misc_feature    2698..2703
                     /label=BsaI Golden recognition
     misc_feature    2705..2744
                     /locus_tag="UNSX"
                     /label=UNSX
     primer_bind     complement(2725..2744)
                     /label=UNSXR
     misc_feature    2745..2774
                     /locus_tag="Extra region of pBR322"
                     /label=Extra region of pBR322
     primer_bind     complement(2745..2774)
                     /label=Loop colony PCR R
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgcattct aggtgattat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      781 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      841 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      901 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      961 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1021 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1081 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1141 gatcaatgtc gatcgtggct ggctcgaaga taccagcaag aatgtcattg cgctgccatt
     1201 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1261 caatggtgac ttctacagcg cggagaatct cactctctcc aggggaagcc gaagtttcca
     1321 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1381 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1441 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1501 tcgatacttc ggcgatcacc gcttccctca tgcgaaacga tcctcatcct gtctcttgat
     1561 cagatattga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     1621 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgcta
     1681 agcttgcatg cctgcaagtc gactctagag gagcggtgat cacaggcagc aacgctctgt
     1741 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     1801 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     1861 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     1921 cgagctcatt actcgcatcc attctcaggc tgtctcgtct cgtctcaggt ctcaaggtcg
     1981 taaaggcgag gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt
     2041 caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact
     2101 gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac
     2161 gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga
     2221 cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga
     2281 tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg
     2341 cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga
     2401 atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa
     2461 agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta
     2521 ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag
     2581 cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga
     2641 gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat aagcttagag
     2701 accaccagga tacatagatt accacaactc cgagcccttc cacccacaga atcaggggat
     2761 aacgcaggaa agaa
//
