LOCUS       L0_EF_Act_ter_-_correct 2590 bp ds-DNA     circular     14-SEP-2023
DEFINITION  .
SOURCE      . (unknown)
  ORGANISM  . .
COMMENT     Imported using the Genbank importer. File name: L0_EF_Act ter.gbk
FEATURES             Location/Qualifiers
     misc_feature    122..122
                     /label="pMB1 high-copy number mutation"
                     /locus_tag="pMB1 high-copy number mutation"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(360..794)
                     /label="pro-CDS-Ter of SpecR"
                     /locus_tag="pro-CDS-Ter of SpecR(1)"
                     /label="pro-CDS-Ter of SpecR(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(360..794)
                     /label="SpecR"
                     /locus_tag="SpecR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(360..794)
                     /label="Spec gene"
                     /locus_tag="Spec gene(1)"
                     /label="Spec gene(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(792..794)
                     /label="BspMI"
                     /locus_tag="BspMI(1)"
                     /label="BspMI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(792..794)
                     /label="BspMI"
                     /locus_tag="BspMI(4)"
                     /label="BspMI(4)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(796..797)
                     /label="BspMI"
                     /locus_tag="BspMI(5)"
                     /label="BspMI(5)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(796..797)
                     /label="BspMI"
                     /locus_tag="BspMI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(796..1151)
                     /label="Spec gene"
                     /locus_tag="Spec gene"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(796..1151)
                     /label="SpecR"
                     /locus_tag="SpecR(1)"
                     /label="SpecR(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(796..1332)
                     /label="pro-CDS-Ter of SpecR"
                     /locus_tag="pro-CDS-Ter of SpecR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    810..828
                     /label="SpecR R"
                     /locus_tag="SpecR R"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(827..846)
                     /label="SpecR F"
                     /locus_tag="SpecR F"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    912..912
                     /label="Mut G->A, silent"
                     /locus_tag="Mut G->A, silent"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(917..922)
                     /label="BpmI"
                     /locus_tag="BpmI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     promoter        complement(1152..1299)
                     /label="specR_promoter_from_pKD13"
                     /locus_tag="specR_promoter_from_pKD13"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    1186..1186
                     /label="Mut C->A"
                     /locus_tag="Mut C->A"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(1314..1316)
                     /label="BspMI"
                     /locus_tag="BspMI(3)"
                     /label="BspMI(3)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(1318..1319)
                     /label="BspMI"
                     /locus_tag="BspMI(2)"
                     /label="BspMI(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    complement(1505..1546)
                     /label="In T-DNA region AH003392.2"
                     /locus_tag="In T-DNA region AH003392.2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     primer          1519..1546
                     /label="Col PCR F"
     misc_feature    1547..1586
                     /label="UNS1"
                     /locus_tag="UNS1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     primer          1554..1577
                     /label="Revised loop col PCR F"
     primer          1576..1593
                     /label="Loop-274"
     misc_feature    complement(1588..1591)
                     /label="BsaI"
                     /locus_tag="BsaI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    1592..1593
                     /label="BsaI recomposition"
                     /locus_tag="BsaI recomposition"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     overhang        1595..1598
                     /label="E"
     terminator      1599..2128
                     /label="act ter"
     misc_feature    2129..2132
                     /label="F"
                     /locus_tag="F"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     primer          complement(2129..2153)
                     /label="Loop-275"
     misc_feature    2133..2133
                     /label="N spacer"
                     /locus_tag="N spacer"
                     /label="N spacer(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    2133..2135
                     /label="BsaI site recomposition"
                     /locus_tag="BsaI site recomposition"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    2136..2139
                     /label="BsaI"
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     misc_feature    2141..2180
                     /label="UNSX"
                     /locus_tag="UNSX"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     primer          complement(2160..2180)
                     /label="Revised Loop Col PCR R"
     misc_feature    2181..2210
                     /label="Extra region of pBR322"
                     /locus_tag="Extra region of pBR322"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
     primer          complement(2181..2210)
                     /label="Col PCR R"
     rep_origin      complement(2271..269)
                     /label="pMB1-pUC19 ORI variant"
                     /note="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
                     /locus_tag="pMB1-pUC19 ORI variant"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {}0}width 5 offset 0"
ORIGIN
        1 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
       61 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
      121 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
      181 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
      241 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
      301 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGCATTCT AGGTGATTAT
      361 TATTTGCCGA CTACCTTGGT GATCTCGCCT TTCACGTAGT GGACAAATTC TTCCAACTGA
      421 TCTGCGCGCG AGGCCAAGCG ATCTTCTTCT TGTCCAAGAT AAGCCTGTCT AGCTTCAAGT
      481 ATGACGGGCT GATACTGGGC CGGCAGGCGC TCCATTGCCC AGTCGGCAGC GACATCCTTC
      541 GGCGCGATTT TGCCGGTTAC TGCGCTGTAC CAAATGCGGG ACAACGTAAG CACTACATTT
      601 CGCTCATCGC CAGCCCAGTC GGGCGGCGAG TTCCATAGCG TTAAGGTTTC ATTTAGCGCC
      661 TCAAATAGAT CCTGTTCAGG AACCGGATCA AAGAGTTCCT CCGCCGCTGG ACCTACCAAG
      721 GCAACGCTAT GTTCTCTTGC TTTTGTCAGC AAGATAGCCA GATCAATGTC GATCGTGGCT
      781 GGCTCGAAGA TACCAGCAAG AATGTCATTG CGCTGCCATT CTCCAAATTG CAGTTCGCGC
      841 TTAGCTGGAT AACGCCACGG AATGATGTCG TCGTGCACAA CAATGGTGAC TTCTACAGCG
      901 CGGAGAATCT CACTCTCTCC AGGGGAAGCC GAAGTTTCCA AAAGGTCGTT GATCAAAGCT
      961 CGCCGCGTTG TTTCATCAAG CCTTACGGTC ACCGTAACCA GCAAATCAAT ATCACTGTGT
     1021 GGCTTCAGGC CGCCATCCAC TGCGGAGCCG TACAAATGTA CGGCCAGCAA CGTCGGTTCG
     1081 AGATGGCGCT CGATGACGCC AACTACCTCT GATAGTTGAG TCGATACTTC GGCGATCACC
     1141 GCTTCCCTCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CAGATATTGA TCCCCTGCGC
     1201 CATCAGATCC TTGGCGGCAA GAAAGCCATC CAGTTTACTT TGCAGGGCTT CCCAACCTTA
     1261 CCAGAGGGCG CCCCAGCTGG CAATTCCGGT TCGCTTGCTA AGCTTGCATG CCTGCAAGTC
     1321 GACTCTAGAG GAGCGGTGAT CACAGGCAGC AACGCTCTGT CATCGTTACA ATCAACATGC
     1381 TACCCTCCGC GAGATCATCC GTGTTTCAAA CCCGGCAGCT TAGTTGCCGT TCTTCCGAAT
     1441 AGCATCGGTA ACATGAGCAA AGTCTGCCGC CTTACAACGG CTCTCCCGCT GACGCCGTCC
     1501 CGGACTGATG GGCTGCCTGT ATCGAGTGGT GATTTTGTGC CGAGCTCATT ACTCGCATCC
     1561 ATTCTCAGGC TGTCTCGTCT CGTCTCAGGT CTCAGCTTGC TACTGAGCTT CATAGTTACA
     1621 GTTGGTTCTT CAACGTTTGT AGGAGGTGTA TTAATCTTTT ATAACGCTGC CTTTATATGT
     1681 TCGCTTTCTT CCAAGTAGAG AATTAACGGA AATCGTCGTC GGTTGAGAAT AACGCAGAAA
     1741 GTCCTTCGTT GTCGAGCCGT AAAGCAGCTG GCTGCTCTCA TTCTAAAAGT TCACGCTCTG
     1801 CACGTGCGAT AACGAGGGGA TACCGTATAC AATCGTCTTT GTACTGGAAA AGATCTTCTC
     1861 AAAATGATTG GAAGAAATCG ATTTCACATA TAAAGGAGCG TTATAAAAGA TTAATACACC
     1921 GCCTACAAAC GTTGAAGAAC CAACTGTAAC TATGAAGCTC AGTAGCTTAG AAGCACTTGC
     1981 GATGAACAAT AGAAGGCCCA GACTCATCGT ACTCTTCCTT CGAGATCCAC ATGCTCTGGA
     2041 AAGTAGAAAG GGAAGCGAGA ATTGACCCTC CAATCCAAAC AGAGTACTTG CGTTCCGGAG
     2101 GAGCAACAAT CTTGACTTTG ATGGAGGCCG CTCGAGACCA CCAGGATACA TAGATTACCA
     2161 CAACTCCGAG CCCTTCCACC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
     2221 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     2281 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     2341 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     2401 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     2461 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     2521 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     2581 GAGTCCAACC
//