LOCUS       plYJP022-int7           4730 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  Plasmid containing integrase for LPattB7 site.
ACCESSION   EU215432
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        71..159
                     /note="Found this feature upstream of what Adam had
                     annotated as the Amp Promoter. We're not sure what it is,
                     but seems harmless so will keep."
                     /X-Transferred_From="pTNS3_YJP440"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Extended Amp Promoter? (<a
                     href=""urn:local:.:50e-g850k2a"">pTNS3_YJP440</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Extended Amp Promoter? (<a
                     href=""urn:local:.:50c-g850k2a"">pE-FLP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Extended Amp Promoter? (<a
                     href=""urn:local:.:50d-g850k2a"">pKD46_LambdaRedMachinery<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Extended Amp Promoter? (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Extended Amp Promoter? (<a
                     href=""urn:local:.:2xp-g850hew"">plYJP053</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Extended Amp Promoter?"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1csu-g8gtbco"">Extended Amp
                     Promoter?</a>"
                     /label="Extended Amp Promoter?"
     promoter        96..187
                     /gene="<i>bla</i>"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pTNS3_YJP440"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:50e-g850k2a"">pTNS3_YJP440</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:50c-g850k2a"">pE-FLP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:50d-g850k2a"">pKD46_LambdaRedMachinery<
                     /a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xp-g850hew"">plYJP053</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:13/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1ct7-g8gtfri"">AmpR promoter</a>"
                     /label="AmpR promoter"
     RBS             188..197
                     /locus_tag="RBS (KanR)"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS
                     (KanR) (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RBS (KanR)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1csk-g8gt3y3"">RBS (KanR)</a>"
                     /label="RBS (KanR)"
     CDS             201..1061
                     /gene="bla"
                     /X-Transferred_From="pTNS3_YJP440"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; bla
                     (<a href=""urn:local:.:50e-g850k2a"">pTNS3_YJP440</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     bla (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     bla (<a href=""urn:local:.:2xp-g850hew"">plYJP053</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ampR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c7h-g8grlwt"">ampR</a>"
                     /label="ampR"
     rep_origin      1465..1846
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K_alternate_seq"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1cjq-g8gs6dm"">R6K_alternate_seq</a>"
                     /label="R6K_alternate_seq"
     OriT            1870..2129
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     protein_bind    2361..2382
                     /bound_moiety="<i>E. coli</i> catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /X-Transferred_From="plYJP022-int7"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; CAP
                     binding site (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTNS3_YJP440"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CAP
                     binding site (<a
                     href=""urn:local:.:1d0f-g8gu1em"">pTNS3_YJP440</a>)"
                     /label="CAP binding site"
     protein_bind    2431..2453
                     /locus_tag="LacO"
                     /X-Transferred_From="plYJP022-int7"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; LacO
                     (<a href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     LacO (<a href=""urn:local:.:2xp-g850hew"">plYJP053</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:4/-2;
                     lac operator (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTNS3_YJP440"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LacO (<a
                     href=""urn:local:.:1d0f-g8gu1em"">pTNS3_YJP440</a>)"
                     /label="LacO"
     misc_feature    2496..2511
                     /locus_tag="BBa prefix"
                     /X-Transferred_From="plYJP022-int7"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; BBa
                     prefix (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa prefix (<a
                     href=""urn:local:.:2xp-g850hew"">plYJP053</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa prefix"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1es0-g8gy3f1"">BBa prefix</a>"
                     /label="BBa prefix"
     promoter        2520..2554
                     /locus_tag="J23101"
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="J23101 (no spacer)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:1bnp-g8gq0io"">J23101 (no
                     spacer)</a>)"
                     /label="J23101"
     regulatory      2560..2634
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     RBS             2635..2653
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0032"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o3-g8gn3ut"">BBa_B0032</a>"
                     /label="BBa_B0032"
     CDS             2654..4042
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="int7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11s8-g8gn3v1"">int7</a>"
                     /label="int7"
     CDS             4424..4492
                     /locus_tag="LacZ alpha"
                     /X-Transferred_From="plYJP022-int7"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; LacZ
                     alpha (<a
                     href=""urn:local:.:2xo-g850hew"">plYJP022-int7</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     LacZ alpha (<a
                     href=""urn:local:.:2xp-g850hew"">plYJP053</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTNS3_YJP440"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LacZ
                     alpha (<a
                     href=""urn:local:.:1d0f-g8gu1em"">pTNS3_YJP440</a>)"
                     /label="LacZ alpha"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagccggtt gtcagccgtt aagtgttcct gtgtcactca
     1501 aaattgcttt gagaggctct aagggcttct cagtgcgtta catccctggc ttgttgtcca
     1561 caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc ttataaaact
     1621 taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt caaacatgag
     1681 agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc
     1741 catgagggtt tagttcgtta aacatgagag cttagtacgt taaacttgag agcttagtac
     1801 gtgaaacatg agagcttagt acgtactatc aacaggttga actgcccatg ttctttcctg
     1861 cgttatcaga gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
     1921 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
     1981 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
     2041 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
     2101 agggttatgc agcggaaagt ataccttaac atgttctttc ctgcgttatc ccctgattct
     2161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2341 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2401 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2461 ggaaacagct atgaccatga ttacgaattc ggcttgcggc cgcttctaga gggcgcgcct
     2521 ttacagctag ctcagtccta ggtattatgc tagccagtta gctgtcaccg gatgtgcttt
     2581 ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt ttaatcacac
     2641 aggaaagtac tagatgaaag tggccattta tgttcgtgtt agcaccgatg aacaggccaa
     2701 agaaggtttt agcattccgg cacagcgtga acgtctgcgt gcattttgtg caagccaggg
     2761 ttgggaaatt gtgcaagaat atattgaaga aggttggagc gcaaaagatc tggatcgtcc
     2821 gcagatgcag cgtctgctga aagatatcaa aaaaggcaac attgatattg tgctggtgta
     2881 tcgtctggat cgcctgaccc gtagcgttct ggatctgtat ctgctgctgc agacctttga
     2941 aaaatacaat gtggcatttc gtagcgccac cgaagtttat gataccagca ccgcaatggg
     3001 tcgtctgttt attaccctgg ttgcagcact ggcacagtgg gaacgtgaaa atctggcaga
     3061 acgtgttaaa tttggtatcg agcagatgat cgatgaaggt aaaaaaccgg gtggtcatag
     3121 cccgtatggt tacaaatttg ataaagactt caattgcacc attattgagg aagaagcaga
     3181 cgttgttcgt atgatctatc gcatgtattg tgatggttat ggctatcgta gcattgcaga
     3241 tcgtctgaat gaactgatgg ttaaaccgcg tattgccaaa gaatggaatc ataatagcgt
     3301 gcgtgatatc ctgaccaacg atatctatat tggcacctat cgttggggtg ataaagttgt
     3361 tccgaataat catccgccta ttattagcga aaccctgttc aaaaaagccc agaaagaaaa
     3421 agaaaaacgt ggcgttgatc gtaaacgcgt tggtaaattt ctgtttaccg gtctgctgca
     3481 gtgtggtaat tgtggtggcc ataaaatgca gggccatttt gataaacgtg agcagaaaac
     3541 ctattaccgt tgtaccaaat gtcaccgcat taccaacgaa aaaaacattc tggaaccgct
     3601 gctggatgaa attcagctgc tgattaccag caaagaatac tttatgagca aattcagcga
     3661 ccgctatgat cagcaagagg ttgttgatgt tagcgcactg acaaaagaac tggaaaaaat
     3721 caaacgccag aaagagaaat ggtacgatct gtatatggat gatcgtaacc cgattccgaa
     3781 agaagaactg tttgccaaaa ttaacgaact gaacaaaaaa gaagaagaaa tctatagcaa
     3841 gctgagcgaa gtggaagaag ataaagaacc ggttgaagag aaatataacc gcctgagcaa
     3901 aatgatcgat tttaaacagc agtttgagca ggccaacgac tttaccaaaa aagagctgct
     3961 gttcagcatc ttcgaaaaga ttgtgattta tcgcgagaaa ggcaagctga aaaaaatcac
     4021 cctggattac accctgaaat aagtggttag gctagctaca tttaatgaca atgtgcaggt
     4081 tgtcatattg gtcatttatt ccgtaactcg ggtcataagg agtggcgtat ttttgtttgg
     4141 tttaatccaa tgcaagaacg gaaatggact cgatttactc atttgccttt attaagtcga
     4201 gctaaggtgg ttaacagtac aacaaagcaa ataaataagg cggatcgtgt gattgagttt
     4261 gaagcatcgg atcttcaacg agccaaaata atcgggggat cctctagagt cgacctgcag
     4321 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4381 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4441 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     4501 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     4561 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4621 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4681 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
