LOCUS       pAJT430                 2987 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  RPU integration plasmid (LPattB7).
ACCESSION   urn.local...1g8k-g8h4kge
VERSION     urn.local...1g8k-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    1..31
                     /X-Transferred_From="attP7 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1eqi-g8gxflt"">attP7 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP7
                     (3') (<a href=""urn:local:.:11us-g8gn3v6"">attP7</a>)"
                     /label="attP7 (3')"
     terminator      32..143
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sg-g8gn3v1"">DT54</a>"
                     /label="DT54"
     rep_origin      235..684
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     OriT            690..949
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     terminator      complement(956..1050)
                     /gene="<br>"
                     /note="transcription terminator from phage lambda"
                     /X-Transferred_From="pAJT447"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lambda
                     t0 terminator (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lambda t0 terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bds-g8gpr46"">lambda t0
                     terminator</a>"
                     /label="lambda t0 terminator"
     CDS             complement(1061..1720)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cmR (cat)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sb-g8gn3v1"">cmR (cat)</a>"
                     /label="cmR (cat)"
     promoter        complement(1721..1823)
                     /note="promoter of the <i>E. coli cat</i> gene encoding
                     chloramphenicol acetyltransferase"
                     /X-Transferred_From="plYJP070-PameR"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; cat
                     promoter (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xf-g850hew"">pAJT420</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y0-g850hew"">plYJP070-PicaR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2wy-g850hew"">pAJT195</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cat promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bul-g8gqln1"">cat promoter</a>"
                     /label="cat promoter"
     protein_bind    1954..1987
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     misc_feature    1988..2033
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     promoter        2055..2069
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xl-g850hew"">pBDF502</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xl-g850hew"">pBDF502</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2xn-g850hew"">pBDF507</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2xm-g850hew"">pBDF506</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pre-PphlF spacer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1er8-g8gxo11"">pre-PphlF spacer</a>"
                     /label="pre-PphlF spacer"
     promoter        2074..2123
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_J23101"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11nd-g8gn3us"">BBa_J23101</a>"
                     /label="BBa_J23101"
     promoter        2089..2123
                     /locus_tag="J23101"
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="J23101 (no spacer)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:1bnp-g8gq0io"">J23101 (no
                     spacer)</a>)"
                     /label="J23101"
     regulatory      2128..2202
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     RBS             2203..2228
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0034"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o4-g8gn3ut"">BBa_B0034</a>"
                     /label="BBa_B0034"
     CDS             2229..2948
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="yfp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11rh-g8gn3v0"">yfp</a>"
                     /label="yfp"
     protein_bind    2953..31
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11us-g8gn3v6"">attP7</a>"
                     /label="attP7"
     protein_bind    2953..2987
                     /X-Transferred_From="attP7 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1eqa-g8gxf5f"">attP7 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP7
                     (5') (<a href=""urn:local:.:11us-g8gn3v6"">attP7</a>)"
                     /label="attP7 (5')"
ORIGIN      
        1 cctaacctta acttttacgc aggttcagct tggaaacaca gaaaaaagcc cgcacctgac
       61 agtgcgggct ttttttttcg accaaaggct cggtaccaaa ttccagaaaa gacacccgaa
      121 agggtgtttt ttcgttttgg tccaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaatgattc
      241 gcacgggccc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      301 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      361 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      421 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      481 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
      541 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
      601 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
      661 ccggacgcat cgtggccgga tcttcttgta gcttatcggc cagcctcgca gagcaggatt
      721 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
      781 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
      841 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
      901 cgctataatg accccgaagc agggttatgc agcggaaagt ataccttaag cttggattct
      961 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1021 aggtcattac tggatctatc aacaggagtc caagcgagct ttacgccccg ccctgccact
     1081 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1141 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1201 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1261 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1321 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1381 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1441 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1501 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1561 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1621 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1681 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1741 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1801 tggaacctct tacgtgccga tcaacgtctc attttcgcca atttaaatcg taattattgg
     1861 ggaccctgta tttagaaaaa taaacaaata ggcatggtct aagaaaccat tattatcatg
     1921 acattaacct ataaaaatag aagttcctat tccgaagttc ctattctcta gaaagtatag
     1981 gaacttcgga tccgaattcg agctccgtcg acactagtgc ggccgcactc gagggcatca
     2041 gagcagattg ggagcgacgt acggtggaag cttgataagt ccctaacttt tacagctagc
     2101 tcagtcctag gtattatgct agcctgaagc tgtcaccgga tgtgctttcc ggtctgatga
     2161 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aatactagag aaagaggaga
     2221 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2281 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2341 ccacctacgg caagctgacc ctgaagttca tctgcaccac aggcaagctg cccgtgccct
     2401 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc
     2461 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2521 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2581 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2641 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2701 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2761 agctcgccga ccactaccag cagaacaccc caatcggcga cggccccgtg ctgctgcccg
     2821 acaaccacta ccttagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2881 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2941 acaagtaagc ttgtgttata aacctgtgtg agagttaagt ttacatg
//
