LOCUS       pAJT475                 3008 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  RPU plasmid w/ high RBS (p15a backbone).
ACCESSION   urn.local...1g8n-g8h4kge
VERSION     urn.local...1g8n-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..46
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     promoter        68..82
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xl-g850hew"">pBDF502</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xl-g850hew"">pBDF502</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-PphlF spacer (<a
                     href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2xn-g850hew"">pBDF507</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pPhlF random spacer (<a
                     href=""urn:local:.:2xm-g850hew"">pBDF506</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pre-PphlF spacer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1er8-g8gxo11"">pre-PphlF spacer</a>"
                     /label="pre-PphlF spacer"
     promoter        87..136
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_J23101"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11nd-g8gn3us"">BBa_J23101</a>"
                     /label="BBa_J23101"
     promoter        102..136
                     /locus_tag="J23101"
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="J23101 (no spacer)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:1bnp-g8gq0io"">J23101 (no
                     spacer)</a>)"
                     /label="J23101"
     regulatory      141..215
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     RBS             216..241
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0034"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o4-g8gn3ut"">BBa_B0034</a>"
                     /label="BBa_B0034"
     CDS             242..961
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="yfp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11rh-g8gn3v0"">yfp</a>"
                     /label="yfp"
     protein_bind    966..1031
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11us-g8gn3v6"">attP7</a>"
                     /label="attP7"
     protein_bind    966..1000
                     /X-Transferred_From="attP7 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1eqa-g8gxf5f"">attP7 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP7
                     (5') (<a href=""urn:local:.:11us-g8gn3v6"">attP7</a>)"
                     /label="attP7 (5')"
     protein_bind    1001..1031
                     /X-Transferred_From="attP7 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1eqi-g8gxflt"">attP7 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP7
                     (3') (<a href=""urn:local:.:11us-g8gn3v6"">attP7</a>)"
                     /label="attP7 (3')"
     terminator      1032..1143
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sg-g8gn3v1"">DT54</a>"
                     /label="DT54"
     CDS             1252..2067
                     /codon_start=1
                     /note="/vntifkey=4"
                     /transl_table=1
                     /Transferred_Translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDN
                     VGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRT
                     PDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFR
                     LAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIF
                     DEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFH
                     LMLDEFF*"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pAJT475"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kanamycin phosphotransferase (<a
                     href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /label="Kanamycin phosphotransferase"
     rep_origin      2139..2975
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="p15a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uy-g8gn3v6"">p15a</a>"
                     /label="p15a"
ORIGIN      
        1 ggatccgaat tcgagctccg tcgacactag tgcggccgca ctcgagggca tcagagcaga
       61 ttgggagcga cgtacggtgg aagcttgata agtccctaac ttttacagct agctcagtcc
      121 taggtattat gctagcctga agctgtcacc ggatgtgctt tccggtctga tgagtccgtg
      181 aggacgaaac agcctctaca aataattttg tttaatacta gagaaagagg agaaatacta
      241 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      301 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      361 cggcaagctg accctgaagt tcatctgcac cacaggcaag ctgcccgtgc cctggcccac
      421 cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa
      481 gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      541 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      601 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      661 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      721 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      781 cgaccactac cagcagaaca ccccaatcgg cgacggcccc gtgctgctgc ccgacaacca
      841 ctaccttagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      901 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      961 agcttgtgtt ataaacctgt gtgagagtta agtttacatg cctaacctta acttttacgc
     1021 aggttcagct tggaaacaca gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg
     1081 accaaaggct cggtaccaaa ttccagaaaa gacacccgaa agggtgtttt ttcgttttgg
     1141 tccctcagta cttgtgtctc aaaatctctg atgtacattg cacaagataa aaatatatca
     1201 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1261 attcaacggg aaacgtcttg ctccaggccg cgattaaatt ccaacatgga tgctgattta
     1321 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
     1381 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1441 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1501 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg
     1561 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1621 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1681 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1741 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1801 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1861 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1921 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1981 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2041 catttgatgc tcgatgagtt tttctaaggt aactgtcaga ccaagtttac tcatatatac
     2101 tttagattga tttttcgttc cactgagcgt cagacccctt aataagatga tcttcttgag
     2161 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
     2221 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
     2281 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
     2341 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
     2401 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
     2461 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     2521 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     2581 gcgcacgagg gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2641 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
     2701 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
     2761 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
     2821 gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt
     2881 gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat
     2941 agtaagccag tatacactcc gctagcgctg aggtctgcct cgtgcagcga gtcagtgagc
     3001 gaggaagc
//
