LOCUS       pAJT194                 3599 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  Type IIS cloning plasmid for LPattB5 integrations w/ OriT.
ACCESSION   urn.local...1g80-g8h4kge
VERSION     urn.local...1g80-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    1..25
                     /X-Transferred_From="attP5 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1ep6-g8gx10h"">attP5 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP5"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP5
                     (3') (<a href=""urn:local:.:11u0-g8gn3v4"">attP5</a>)"
                     /label="attP5 (3')"
     terminator      26..137
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sg-g8gn3v1"">DT54</a>"
                     /label="DT54"
     rep_origin      229..678
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     OriT            684..943
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     terminator      1003..1142
                     /created_by="alextriassi"
                     /X-Transferred_From="pAJT194"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; DT60
                     new (<a href=""urn:local:.:2wx-g850hew"">pAJT194</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT60 new"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bcv-g8gpo4j"">DT60 new</a>"
                     /label="DT60 new"
     promoter        1143..1171
                     /locus_tag="Pcon"
                     /X-Transferred_From="plYJP072"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pcon
                     (<a href=""urn:local:.:2y4-g850hew"">plYJP072</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Pcon (<a href=""urn:local:.:2xd-g850hew"">pAJT418</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Pcon (<a href=""urn:local:.:2xq-g850hew"">plYJP064</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Pcon (<a href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Pcon (<a href=""urn:local:.:2wx-g850hew"">pAJT194</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Pcon (<a href=""urn:local:.:2xm-g850hew"">pBDF506</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="constitutive promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pcon (<a
                     href=""urn:local:.:1bt8-g8gqkux"">constitutive
                     promoter</a>)"
                     /label="Pcon"
     CDS             1291..2409
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tetA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11s9-g8gn3v1"">tetA</a>"
                     /label="tetA"
     protein_bind    2516..2549
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     misc_feature    2550..2595
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     promoter        2611..2645
                     /locus_tag="J23101"
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="J23101 (no spacer)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:1bnp-g8gq0io"">J23101 (no
                     spacer)</a>)"
                     /label="J23101"
     regulatory      2651..2725
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     RBS             2726..2744
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0032"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o3-g8gn3ut"">BBa_B0032</a>"
                     /label="BBa_B0032"
     CDS             2745..3461
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="sfgfp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ri-g8gn3v0"">sfgfp</a>"
                     /label="sfgfp"
     terminator      3470..3549
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0010"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11se-g8gn3v1"">BBa_B0010</a>"
                     /label="BBa_B0010"
     protein_bind    3565..25
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP5"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11u0-g8gn3v4"">attP5</a>"
                     /label="attP5"
     protein_bind    3565..3599
                     /X-Transferred_From="attP5 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1ep2-g8gx0d8"">attP5 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP5"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP5
                     (5') (<a href=""urn:local:.:11u0-g8gn3v4"">attP5</a>)"
                     /label="attP5 (5')"
ORIGIN      
        1 ttgacaactt gcgcaccctg atctgggaaa cacagaaaaa agcccgcacc tgacagtgcg
       61 ggcttttttt ttcgaccaaa ggctcggtac caaattccag aaaagacacc cgaaagggtg
      121 ttttttcgtt ttggtccagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaatg attcgcacgg
      241 gcccatggct aattcccatg tcagccgtta agtgttcctg tgtcactcaa aattgctttg
      301 agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac aaccgttaaa
      361 ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt aaaaccttag
      421 aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga gcttagtacg
      481 tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc atgagggttt
      541 agttcgttaa acatgagagc ttagtacgtt aaacatgaga gcttagtacg tgaaacatga
      601 gagcttagta cgtactatca acaggttgaa ctgctgatct tcagatcctc tacgccggac
      661 gcatcgtggc cggatcttct tgtagcttat cggccagcct cgcagagcag gattcccgtt
      721 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
      781 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
      841 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
      901 aatgaccccg aagcagggtt atgcagcgga aagtatacct taagcttgga ttctcaccaa
      961 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc caacatttaa taaaaaaagg
     1021 gcggtcgcaa gatcgccctt ttttacgtat gacacctcgt ctcaaccaaa gcaatcaacc
     1081 agtgaaaaat ggcgcccatc ggcgccattt ttttatggtt ccttatcatc tggcgaatcg
     1141 gattgacagc ttatcatcga taaactgtaa tgcggtagtt tatcacagtt aaattgctaa
     1201 cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
     1261 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     1321 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     1381 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
     1441 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
     1501 gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     1561 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     1621 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     1681 ggcgccatct ccctgcacgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     1741 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgtcc gatgcccttg
     1801 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgaccat tgtggccgca
     1861 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     1921 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     1981 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     2041 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     2101 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     2161 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     2221 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     2281 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     2341 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     2401 tcgacctgag acaattgtct aattaattgc ggaccctaga ggtccccttt tttattttaa
     2461 aaattttttc acaaaacggt ttacaagcat aaaatctctg agaagttcct attccgaagt
     2521 tcctattctc tagaaagtat aggaacttcg gatccgaatt cgagctccgt cgacactagt
     2581 gcggccgcac tcgaggtccc gagaccatca tttacagcta gctcagtcct aggtattatg
     2641 ctagccagtt agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac
     2701 agcctctaca aataattttg tttaatcaca caggaaagta ctagatgagc aaaggagaag
     2761 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     2821 aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat
     2881 ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct
     2941 atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga
     3001 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct
     3061 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
     3121 agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag tacaacttta
     3181 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
     3241 aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata
     3301 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
     3361 tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
     3421 ctgctgggat tacacatggc atggatgagc tctacaaata atactagagc caggcatcaa
     3481 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3541 aacgctctca tgtggtctca cgctccctaa tacgcaagtc gataactctc ctgggagcg
//
