LOCUS       pAJT195                 3056 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  Type IIS cloning plasmid for LPattB7 integrations w/ OriT.
ACCESSION   urn.local...1g81-g8h4kge
VERSION     urn.local...1g81-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    1..31
                     /X-Transferred_From="attP7 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1eqi-g8gxflt"">attP7 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP7
                     (3') (<a href=""urn:local:.:11us-g8gn3v6"">attP7</a>)"
                     /label="attP7 (3')"
     terminator      32..143
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sg-g8gn3v1"">DT54</a>"
                     /label="DT54"
     rep_origin      235..684
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     OriT            690..949
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     terminator      complement(956..1050)
                     /gene="<br>"
                     /note="transcription terminator from phage lambda"
                     /X-Transferred_From="pAJT447"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lambda
                     t0 terminator (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lambda t0 terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bds-g8gpr46"">lambda t0
                     terminator</a>"
                     /label="lambda t0 terminator"
     CDS             complement(1061..1720)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cmR (cat)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sb-g8gn3v1"">cmR (cat)</a>"
                     /label="cmR (cat)"
     promoter        complement(1721..1823)
                     /note="promoter of the <i>E. coli cat</i> gene encoding
                     chloramphenicol acetyltransferase"
                     /X-Transferred_From="plYJP070-PameR"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; cat
                     promoter (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2xf-g850hew"">pAJT420</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2y0-g850hew"">plYJP070-PicaR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     cat promoter (<a
                     href=""urn:local:.:2wy-g850hew"">pAJT195</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cat promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bul-g8gqln1"">cat promoter</a>"
                     /label="cat promoter"
     protein_bind    1954..1987
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     misc_feature    1988..2033
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     promoter        2067..2101
                     /locus_tag="J23101"
                     /X-Transferred_From="pAJT430"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:2xh-g850hew"">pAJT430</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xg-g850hew"">pAJT429</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2xk-g850hew"">pAJT475</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     J23101 (<a href=""urn:local:.:2x3-g850hew"">pAJT248</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="J23101 (no spacer)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; J23101
                     (<a href=""urn:local:.:1bnp-g8gq0io"">J23101 (no
                     spacer)</a>)"
                     /label="J23101"
     regulatory      2107..2181
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     RBS             2182..2200
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0032"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o3-g8gn3ut"">BBa_B0032</a>"
                     /label="BBa_B0032"
     CDS             2201..2917
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="sfgfp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ri-g8gn3v0"">sfgfp</a>"
                     /label="sfgfp"
     terminator      2926..3005
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0010"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11se-g8gn3v1"">BBa_B0010</a>"
                     /label="BBa_B0010"
     protein_bind    3022..31
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11us-g8gn3v6"">attP7</a>"
                     /label="attP7"
     protein_bind    3022..3056
                     /X-Transferred_From="attP7 (split)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1eqa-g8gxf5f"">attP7 (split)</a>"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP7
                     (5') (<a href=""urn:local:.:11us-g8gn3v6"">attP7</a>)"
                     /label="attP7 (5')"
ORIGIN      
        1 cctaacctta acttttacgc aggttcagct tggaaacaca gaaaaaagcc cgcacctgac
       61 agtgcgggct ttttttttcg accaaaggct cggtaccaaa ttccagaaaa gacacccgaa
      121 agggtgtttt ttcgttttgg tccaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaatgattc
      241 gcacgggccc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      301 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      361 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      421 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      481 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
      541 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
      601 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
      661 ccggacgcat cgtggccgga tcttcttgta gcttatcggc cagcctcgca gagcaggatt
      721 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
      781 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
      841 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
      901 cgctataatg accccgaagc agggttatgc agcggaaagt ataccttaag cttggattct
      961 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1021 aggtcattac tggatctatc aacaggagtc caagcgagct ttacgccccg ccctgccact
     1081 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1141 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1201 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1261 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1321 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1381 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1441 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1501 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1561 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1621 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1681 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1741 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1801 tggaacctct tacgtgccga tcaacgtctc attttcgcca atttaaatcg taattattgg
     1861 ggaccctgta tttagaaaaa taaacaaata ggcatggtct aagaaaccat tattatcatg
     1921 acattaacct ataaaaatag aagttcctat tccgaagttc ctattctcta gaaagtatag
     1981 gaacttcgga tccgaattcg agctccgtcg acactagtgc ggccgcactc gagggcatca
     2041 gagcagattg ggaggtgtct tcatcattta cagctagctc agtcctaggt attatgctag
     2101 ccagttagct gtcaccggat gtgctttccg gtctgatgag tccgtgagga cgaaacagcc
     2161 tctacaaata attttgttta atcacacagg aaagtactag atgagcaaag gagaagaact
     2221 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     2281 ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat
     2341 ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg
     2401 tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc
     2461 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa
     2521 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg
     2581 tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc
     2641 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
     2701 tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc
     2761 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct
     2821 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
     2881 tgggattaca catggcatgg atgagctcta caaataatac tagagccagg catcaaataa
     2941 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     3001 ctctcatgtg aagacatgct tgtgttataa acctgtgtga gagttaagtt tacatg
//
