LOCUS       pAJT237-Ptac            3055 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  PTac-YFP plasmid for LPattB2 integration.
ACCESSION   urn.local...1g83-g8h4kge
VERSION     urn.local...1g83-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    complement(1..27)
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (5')"
     CDS             complement(28..747)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="yfp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11rh-g8gn3v0"">yfp</a>"
                     /label="yfp"
     RBS             complement(748..773)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0034"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o4-g8gn3ut"">BBa_B0034</a>"
                     /label="BBa_B0034"
     regulatory      complement(774..848)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     promoter        complement(849..919)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PTac"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11mh-g8gn3uq"">PTac</a>"
                     /label="PTac"
     misc_feature    complement(905..919)
                     /locus_tag="pre-pTac random space"
                     /X-Transferred_From="pAJT419"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xd-g850hew"">pAJT418</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xf-g850hew"">pAJT420</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pre-pTac random spacer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1f2f-g8h0q8q"">pre-pTac random
                     spacer</a>"
                     /label="pre-pTac random spacer"
     misc_feature    954..999
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     protein_bind    complement(1000..1033)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     promoter        1099..1177
                     /gene="<i>bla</i>"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter_truncated"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1db7-g8gukjp"">AmpR
                     promoter_truncated</a>"
                     /label="AmpR promoter_truncated"
     RBS             1178..1187
                     /locus_tag="RBS (KanR)"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS
                     (KanR) (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RBS (KanR)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1csk-g8gt3y3"">RBS (KanR)</a>"
                     /label="RBS (KanR)"
     CDS             1191..2006
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="kanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sa-g8gn3v1"">kanR</a>"
                     /label="kanR"
     terminator      2017..2111
                     /gene="<br>"
                     /note="transcription terminator from phage lambda"
                     /X-Transferred_From="pAJT447"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lambda
                     t0 terminator (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lambda t0 terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bds-g8gpr46"">lambda t0
                     terminator</a>"
                     /label="lambda t0 terminator"
     OriT            2118..2377
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     rep_origin      complement(2383..2832)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     terminator      complement(2908..3019)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sg-g8gn3v1"">DT54</a>"
                     /label="DT54"
     protein_bind    complement(3020..27)
                     /created_by="alextriassi"
                     /X-Transferred_From="attP2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11u4-g8gn3v5"">attP2</a>"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1esu-g8gyn88"">attP2</a>"
                     /label="attP2"
     protein_bind    complement(3020..3055)
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (3')"
ORIGIN      
        1 gaatctcgcg tagacacata catgagctta cttgtacagc tcgtccatgc cgagagtgat
       61 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      121 gctcagggcg gactggtagc taaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      181 gattggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      241 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
      301 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      361 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      421 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      481 catggcggac ttgaagaagt cgtgcagctt catgtggtcg gggtagcggg cgaagcattg
      541 caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgcctgtggt
      601 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      661 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      721 gaacagctcc tcgcccttgc tcaccatcta gtatttctcc tctttctcta gtattaaaca
      781 aaattatttg tagaggctgt ttcgtcctca cggactcatc agaccggaaa gcacatccgg
      841 tgacagctaa ttgtgagcgc tcacaattcc acacattata cgagccgatg attaattgtc
      901 aacacagcca acgatcgttc tcctggtctt ccagtacaat ctgctctgat gccctcgagt
      961 gcggccgcac tagtgtcgac ggagctcgaa ttcggatccg aagttcctat actttctaga
     1021 gaataggaac ttcggaatag gaacttctat ttttataggt taatgtcatg ataataatgg
     1081 tttcttagac catgcctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1141 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagccata
     1201 ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat
     1261 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     1321 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     1381 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     1441 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
     1501 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     1561 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     1621 accgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     1681 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     1741 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     1801 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1861 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     1921 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     1981 atttgatgct cgatgagttt ttctaaagct cgcttggact cctgttgata gatccagtaa
     2041 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     2101 ttggtgagaa tccaagctta aggtatactt tccgctgcat aaccctgctt cggggtcatt
     2161 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     2221 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     2281 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     2341 aacgggaatc ctgctctgcg aggctggccg ataagctaca agaagatccg gccacgatgc
     2401 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     2461 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     2521 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     2581 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     2641 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
     2701 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
     2761 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt agccatgggc
     2821 ccgtgcgaat catttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2881 cctgcgtgga taaccgtatt accgcctgga ccaaaacgaa aaaacaccct ttcgggtgtc
     2941 ttttctggaa tttggtaccg agcctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
     3001 ggcttttttc tgtgtttcca gccaagagca gtgccttgca gaagttctcg ggcga
//
