LOCUS       pAJT238-Pbad            3078 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  PBad-YFP plasmid for LPattB2 integration.
ACCESSION   urn.local...1g84-g8h4kge
VERSION     urn.local...1g84-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    complement(1..27)
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (5')"
     CDS             complement(28..747)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="yfp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11rh-g8gn3v0"">yfp</a>"
                     /label="yfp"
     RBS             complement(748..773)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BBa_B0034"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o4-g8gn3ut"">BBa_B0034</a>"
                     /label="BBa_B0034"
     regulatory      complement(774..848)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ00"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11pw-g8gn3ux"">RiboJ00</a>"
                     /label="RiboJ00"
     promoter        complement(849..941)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PBadmc"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11mg-g8gn3uq"">PBadmc</a>"
                     /label="PBadmc"
     misc_feature    complement(927..941)
                     /locus_tag="pre-pTac random space"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pAJT419"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pre-pTac
                     random space (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xd-g850hew"">pAJT418</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xf-g850hew"">pAJT420</a>)"
                     /modified_by="alextriassi"
                     /label="pre-pTac random spacer"
     misc_feature    976..1021
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     protein_bind    complement(1022..1055)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     promoter        1121..1199
                     /gene="<i>bla</i>"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter_truncated"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1db7-g8gukjp"">AmpR
                     promoter_truncated</a>"
                     /label="AmpR promoter_truncated"
     RBS             1200..1209
                     /locus_tag="RBS (KanR)"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS
                     (KanR) (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RBS (KanR)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1csk-g8gt3y3"">RBS (KanR)</a>"
                     /label="RBS (KanR)"
     CDS             1213..2028
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="kanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sa-g8gn3v1"">kanR</a>"
                     /label="kanR"
     terminator      2039..2133
                     /gene="<br>"
                     /note="transcription terminator from phage lambda"
                     /X-Transferred_From="pAJT447"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lambda
                     t0 terminator (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lambda t0 terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bds-g8gpr46"">lambda t0
                     terminator</a>"
                     /label="lambda t0 terminator"
     OriT            2140..2399
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     rep_origin      complement(2405..2854)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     terminator      complement(2931..3042)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sg-g8gn3v1"">DT54</a>"
                     /label="DT54"
     protein_bind    complement(3043..27)
                     /created_by="alextriassi"
                     /X-Transferred_From="attP2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11u4-g8gn3v5"">attP2</a>"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1esu-g8gyn88"">attP2</a>"
                     /label="attP2"
     protein_bind    complement(3043..3078)
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (3')"
ORIGIN      
        1 gaatctcgcg tagacacata catgagctta cttgtacagc tcgtccatgc cgagagtgat
       61 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      121 gctcagggcg gactggtagc taaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      181 gattggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      241 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
      301 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      361 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      421 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      481 catggcggac ttgaagaagt cgtgcagctt catgtggtcg gggtagcggg cgaagcattg
      541 caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgcctgtggt
      601 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      661 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      721 gaacagctcc tcgcccttgc tcaccatcta gtatttctcc tctttctcta gtattaaaca
      781 aaattatttg tagaggctgt ttcgtcctca cggactcatc agaccggaaa gcacatccgg
      841 tgacagctcg ggtatggaga aaaatataga gagttgcgat aaaaagcgtc aggtaggatc
      901 cgctaatctt atggataaaa atgctacagc caacgatcgt tctcctggtc ttccagtaca
      961 atctgctctg atgccctcga gtgcggccgc actagtgtcg acggagctcg aattcggatc
     1021 cgaagttcct atactttcta gagaatagga acttcggaat aggaacttct atttttatag
     1081 gttaatgtca tgataataat ggtttcttag accatgccta tttgtttatt tttctaaata
     1141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1201 aaaaggaaga gtatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat
     1261 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1321 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1381 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1441 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1501 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
     1561 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1621 tgtaattgtc cttttaacag cgaccgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1681 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1741 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     1801 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     1861 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     1921 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     1981 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaag ctcgcttgga
     2041 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     2101 ggttgccgcc gggcgttttt tattggtgag aatccaagct taaggtatac tttccgctgc
     2161 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     2221 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     2281 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     2341 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgataagcta
     2401 caagaagatc cggccacgat gcgtccggcg tagaggatct gaagatcagc agttcaacct
     2461 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact
     2521 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa
     2581 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat
     2641 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat
     2701 aaggctttta aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca
     2761 ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct
     2821 gacatgggaa ttagccatgg gcccgtgcga atcattttta cggttcctgg ccttttgctg
     2881 gccttttgct cacatgttct ttcctgcgtg gataaccgta ttaccgcctg ggaccaaaac
     2941 gaaaaaacac cctttcgggt gtcttttctg gaatttggta ccgagccttt ggtcgaaaaa
     3001 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt ccagccaaga gcagtgcctt
     3061 gcagaagttc tcgggcga
//
