LOCUS       pAJT249-psrA            3051 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  psrA repressor plasmid for LPattB2 integration.
ACCESSION   urn.local...1g87-g8h4kge
VERSION     urn.local...1g87-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    1..36
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (3')"
     rep_origin      128..577
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     OriT            583..842
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     terminator      complement(849..943)
                     /gene="<br>"
                     /note="transcription terminator from phage lambda"
                     /X-Transferred_From="pAJT447"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lambda
                     t0 terminator (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lambda t0 terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bds-g8gpr46"">lambda t0
                     terminator</a>"
                     /label="lambda t0 terminator"
     CDS             complement(954..1769)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="kanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sa-g8gn3v1"">kanR</a>"
                     /label="kanR"
     RBS             complement(1773..1782)
                     /locus_tag="RBS (KanR)"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS
                     (KanR) (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RBS (KanR)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1csk-g8gt3y3"">RBS (KanR)</a>"
                     /label="RBS (KanR)"
     promoter        complement(1783..1861)
                     /gene="<i>bla</i>"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter_truncated"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1db7-g8gukjp"">AmpR
                     promoter_truncated</a>"
                     /label="AmpR promoter_truncated"
     protein_bind    1927..1960
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     misc_feature    1961..2006
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     promoter        2033..2103
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PTac"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11mh-g8gn3uq"">PTac</a>"
                     /label="PTac"
     misc_feature    2033..2047
                     /locus_tag="pre-pTac random space"
                     /X-Transferred_From="pAJT419"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xd-g850hew"">pAJT418</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xf-g850hew"">pAJT420</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pre-pTac random spacer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1f2f-g8h0q8q"">pre-pTac random
                     spacer</a>"
                     /label="pre-pTac random spacer"
     regulatory      2104..2182
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ScmJ"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11p3-g8gn3uv"">ScmJ</a>"
                     /label="ScmJ"
     RBS             2183..2216
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o5-g8gn3ut"">R1</a>"
                     /label="R1"
     CDS             2217..2930
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="psrA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11qo-g8gn3uy"">psrA</a>"
                     /label="psrA"
     terminator      2931..3020
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT82"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11t5-g8gn3v3"">DT82</a>"
                     /label="DT82"
     protein_bind    3025..36
                     /created_by="alextriassi"
                     /X-Transferred_From="attP2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11u4-g8gn3v5"">attP2</a>"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1esu-g8gyn88"">attP2</a>"
                     /label="attP2"
     protein_bind    3025..3051
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (5')"
ORIGIN      
        1 tcgcccgaga acttctgcaa ggcactgctc ttggctaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      121 gtaaaaatga ttcgcacggg cccatggcta attcccatgt cagccgttaa gtgttcctgt
      181 gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca tccctggctt
      241 gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct tttttttctt
      301 ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca
      361 aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt
      421 acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag
      481 cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac tgctgatctt
      541 cagatcctct acgccggacg catcgtggcc ggatcttctt gtagcttatc ggccagcctc
      601 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
      661 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
      721 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
      781 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa agtatacctt
      841 aagcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      901 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctttagaaa
      961 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     1021 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     1081 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     1141 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     1201 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     1261 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     1321 gcgagacgaa atacgcggtc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     1381 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     1441 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     1501 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     1561 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     1621 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     1681 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     1741 caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta ttgaagcatt
     1801 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1861 ataggcatgg tctaagaaac cattattatc atgacattaa cctataaaaa tagaagttcc
     1921 tattccgaag ttcctattct ctagaaagta taggaacttc ggatccgaat tcgagctccg
     1981 tcgacactag tgcggccgca ctcgagggca tcagagcaga ttgtactgac taaacgatcg
     2041 ttggctgtgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcgctcaca
     2101 attagcgctg tctgtacttg tatcagtaca ctgacgagtc cctaaaggac gaaacaccgc
     2161 ctctacaaat aattttgttt aatttaattc gcggaagcgc agagataagg ggtatcatgg
     2221 cacagagcga aaccgttgaa cgtattctgg atgtagcaga acagctgttt gcagaacgtg
     2281 gttttgcaga aaccagcctg cgtctgatta ccagcaaagc cggtgttaat ctggcagcag
     2341 tgaattatca ttttggcagc aaaaaagcac tgattcaggc agtttttagc cgttttctgg
     2401 gtccgttttg tgcaagcctg gaacgtgaac tggaacgtcg tcaggcacgt ccggaacaga
     2461 aaccgagcct ggaagaactg ctggaaatgc tggttgaaca ggcactggca gttcagcctc
     2521 gtagcaataa tgatctgagc atttttatgt gtctgctggg tctggcattt agccagagcc
     2581 agggtcatct gcgtcgttat ctggaagata tgtatggtaa agtgttccgt cgttatatgc
     2641 tgctggttaa tgaagcagca ccgcgtgttc cgcctctgga actgttttgg cgtgttcatt
     2701 ttatgctggg tgcagcagca tttagcatga gcggtattaa agcactgcgt gcaattgcag
     2761 aaaccgattt tggtattaac accagcattg aacaggttat gcgtctgatg gttccgtttc
     2821 tggcagcagg tatgcgtgca gatagcggtg ttaccgatga agcaatggca gcagcacagc
     2881 tgcgtccgcg tagcaaaacc agcaccagcg caaccaccgc aaaagcataa cttattccat
     2941 aacaaagccg ggtaattccc ggctttgttg tatctgaaca ataaatggat gccctgcgta
     3001 agcggggcat ttttcttcct tgccgctcat gtatgtgtct acgcgagatt c
//
