LOCUS       pAJT255-ameRS           2995 bp    DNA     circular UNA 06-MAY-2023
DEFINITION  ameRS repressor plasmid for LPattB2 integration.
ACCESSION   urn.local...1g8c-g8h4kge
VERSION     urn.local...1g8c-g8h4kge
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     protein_bind    complement(1..27)
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (5') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (5') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (5')"
     terminator      complement(32..121)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="DT60"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11t3-g8gn3v3"">DT60</a>"
                     /label="DT60"
     CDS             complement(122..781)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ameRs"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11rd-g8gn3v0"">ameRs</a>"
                     /label="ameRs"
     RBS             complement(782..817)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11o9-g8gn3ut"">F2</a>"
                     /label="F2"
     regulatory      complement(818..892)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RiboJ54"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ps-g8gn3ux"">RiboJ54</a>"
                     /label="RiboJ54"
     promoter        complement(893..963)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PTac"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11mh-g8gn3uq"">PTac</a>"
                     /label="PTac"
     misc_feature    complement(949..963)
                     /locus_tag="pre-pTac random space"
                     /X-Transferred_From="pAJT419"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xd-g850hew"">pAJT418</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pre-pTac random space (<a
                     href=""urn:local:.:2xf-g850hew"">pAJT420</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pre-pTac random spacer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1f2f-g8h0q8q"">pre-pTac random
                     spacer</a>"
                     /label="pre-pTac random spacer"
     misc_feature    990..1035
                     /locus_tag="MCS"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; MCS
                     (<a href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xv-g850hew"">plYJP070-PameR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y1-g850hew"">plYJP070-PlitR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xw-g850hew"">plYJP070-PamtR2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y2-g850hew"">plYJP070-PphlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MCS (<a
                     href=""urn:local:.:2y3-g850hew"">plYJP070-PpsrA</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1equ-g8gxmes"">MCS</a>"
                     /label="MCS"
     protein_bind    complement(1036..1069)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11uw-g8gn3v6"">FRT</a>"
                     /label="FRT"
     promoter        1135..1213
                     /gene="<i>bla</i>"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter_truncated"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1db7-g8gukjp"">AmpR
                     promoter_truncated</a>"
                     /label="AmpR promoter_truncated"
     RBS             1214..1223
                     /locus_tag="RBS (KanR)"
                     /X-Transferred_From="pAJT258-phlF"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS
                     (KanR) (<a
                     href=""urn:local:.:2xc-g850hew"">pAJT258-phlF</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xb-g850hew"">pAJT257-betI</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xe-g850hew"">pAJT419</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x9-g850hew"">pAJT255-ameRS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x7-g850hew"">pAJT252-amtR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2x8-g850hew"">pAJT254-litR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     RBS (KanR) (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RBS (KanR)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1csk-g8gt3y3"">RBS (KanR)</a>"
                     /label="RBS (KanR)"
     CDS             1227..2042
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="kanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11sa-g8gn3v1"">kanR</a>"
                     /label="kanR"
     terminator      2053..2147
                     /gene="<br>"
                     /note="transcription terminator from phage lambda"
                     /X-Transferred_From="pAJT447"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lambda
                     t0 terminator (<a
                     href=""urn:local:.:2xi-g850hew"">pAJT447</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2wz-g850hew"">pAJT196</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     lambda t0 terminator (<a
                     href=""urn:local:.:2xj-g850hew"">pAJT448</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lambda t0 terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1bds-g8gpr46"">lambda t0
                     terminator</a>"
                     /label="lambda t0 terminator"
     OriT            2154..2413
                     /locus_tag="oriT"
                     /note="oriT"
                     /modified_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:18nn-g8gobqi"">oriT</a>"
                     /label="oriT"
     rep_origin      complement(2419..2868)
                     /created_by="alextriassi"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="R6K"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11ux-g8gn3v6"">R6K</a>"
                     /label="R6K"
     protein_bind    complement(2960..27)
                     /created_by="alextriassi"
                     /X-Transferred_From="attP2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:11u4-g8gn3v5"">attP2</a>"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1esu-g8gyn88"">attP2</a>"
                     /label="attP2"
     protein_bind    complement(2960..2995)
                     /locus_tag="attP2"
                     /X-Transferred_From="plYJP067-PvanCC_v2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a
                     href=""urn:local:.:2xu-g850hew"">plYJP067-PvanCC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xt-g850hew"">plYJP067-Pttg</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xr-g850hew"">plYJP067-PcymRC_v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP2 (3') (<a
                     href=""urn:local:.:2xs-g850hew"">plYJP067-Ptet</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP2
                     (3') (<a href=""urn:local:.:1esu-g8gyn88"">attP2</a>)"
                     /label="attP2 (3')"
ORIGIN      
        1 gaatctcgcg tagacacata catgagcagc gcataaaaaa atggcgccga tgggcgccat
       61 ttttcactgt gtcatacgta aaaaagggcg atcttgcgac cgcccttttt ttattaaatg
      121 tttattttgc cagcggatta cgcagacctg catcaatcag atcaaccagc tgatcacaac
      181 gggttgccag attaccaata tcttcttgtg caatcagaac cggatgaata acgctggtca
      241 gtgcatgcag aacggtttct gccagaaccg gaatatcggt tgcaatatac agacctgctt
      301 caacaccatc acgaataatt tcggccagca gttttgcaat cacgcttttg taataatcgc
      361 cacatttcat atcctgtttg gcggtcatca ggatctgaat aaaaacccgt atgtgtttga
      421 aatgatcctg atgatgctgt tccatcagat tacgtgccag atgacgcaga cgatccagcg
      481 gtgcatggct tttatccagc ggacaaatct gacgttcaaa aacaccaatc tgaccaaaac
      541 caattgcatc aaccagtgcg tttttgctgc taaaatgttt aaacacattt gccggactca
      601 tattcagtgc gcttgcaata tctgcaatgg caactgcatt aaaaccacgt tcactaaaca
      661 gttcttcggc ttttgccaga atatcacgac gggtttcttc ggcactatga cgcggacgac
      721 gacgaaccgg cagatcgctt ttacgatcac ctttacgcac ctgatcaatg gttttgttca
      781 tctattagct cctgtttgaa acatagtcca tagcgtatta aacaaaatta tttgtagagg
      841 gggtttcgtc atcactgact catcagagtt gaaagcacat caactgaccc ctaattgtga
      901 gcgctcacaa ttccacacat tatacgagcc gatgattaat tgtcaacaca gccaacgatc
      961 gttctcccag tacaatctgc tctgatgccc tcgagtgcgg ccgcactagt gtcgacggag
     1021 ctcgaattcg gatccgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
     1081 ttctattttt ataggttaat gtcatgataa taatggtttc ttagaccatg cctatttgtt
     1141 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1201 ttcaataata ttgaaaaagg aagagtatga gccatattca acgggaaacg tcttgctcta
     1261 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1321 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     1381 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1441 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1501 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     1561 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1621 attcgattcc tgtttgtaat tgtcctttta acagcgaccg cgtatttcgt ctcgctcagg
     1681 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     1741 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     1801 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     1861 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1921 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1981 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2041 aaagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     2101 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcttaaggt
     2161 atactttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     2221 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     2281 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     2341 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     2401 tggccgataa gctacaagaa gatccggcca cgatgcgtcc ggcgtagagg atctgaagat
     2461 cagcagttca acctgttgat agtacgtact aagctctcat gtttcacgta ctaagctctc
     2521 atgtttaacg tactaagctc tcatgtttaa cgaactaaac cctcatggct aacgtactaa
     2581 gctctcatgg ctaacgtact aagctctcat gtttcacgta ctaagctctc atgtttgaac
     2641 aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt tttataagaa
     2701 aaaaaagaat atataaggct tttaaagctt ttaaggttta acggttgtgg acaacaagcc
     2761 agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttga gtgacacagg
     2821 aacacttaac ggctgacatg ggaattagcc atgggcccgt gcgaatcatt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgccta gccaagagca gtgccttgca gaagttctcg ggcga
//
