LOCUS       Exported                9091 bp ds-DNA     circular SYN 12-MAR-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   David Bryant
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated MMLV gag gene lacking the start codon"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2259..4556
                     /codon_start=1
                     /label=EGFP-hPODXL
                     /translation="MRCALALSALLLLLSTPPLLPSMVSKGEELFTGVVPILVELDGDV
                     NGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQH
                     DFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHK
                     LEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSPSPSPSPSQNATQTTTDSS
                     NKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSG
                     PVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGG
                     KSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLMKISSSSSTV
                     AIPGYTFTSPGMTTTLPSSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTL
                     PETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAG
                     GASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERL
                     KDKWDELKEAGVSDMKLGDQGPPEEAEDRFSMPLIITIVCMASFLLLVAALYGCCHQRL
                     SQRKDQQRLTEELQTVENGYHDNPTLEVMETSSEMQEKKVVSLNGELGDSWIVPLDNLT
                     KDDLDEEEDTHL"
     misc_feature    4572..5144
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
     CDS             5203..5577
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5797..6222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        6488..6817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6668..6803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(7110..7698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7869..8729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8730..8834)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(9020..9091,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgctgcagga attgatccgc ggccgcaccg gtgccaccat gcgctgcgcg ctggcgctct
     2281 cggcgctgct gctactgttg tcaacgccgc cgctgctgcc gtcgatggtg agcaagggcg
     2341 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2401 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2461 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2521 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2581 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     2641 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2701 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2761 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2821 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2881 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2941 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3001 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtcgccgtcg ccgtcgccgt
     3061 cgccctccca gaatgcaacc cagactacta cggactcatc taacaaaaca gcaccgactc
     3121 cagcatccag tgtcaccatc atggctacag atacagccca gcagagcaca gtccccactt
     3181 ccaaggccaa cgaaatcttg gcctcggtca aggcgaccac ccttggtgta tccagtgact
     3241 caccggggac tacaaccctg gctcagcaag tctcaggccc agtcaacact accgtggcta
     3301 gaggaggcgg ctcaggcaac cctactacca ccatcgagag ccccaagagc acaaaaagtg
     3361 cagacaccac tacagttgca acctccacag ccacagctaa acctaacacc acaagcagcc
     3421 agaatggagc agaagataca acaaactctg gggggaaaag cagccacagt gtgaccacag
     3481 acctcacatc cactaaggca gaacatctga cgacccctca ccctacaagt ccacttagcc
     3541 cccgacaacc cacttcgacg catcctgtgg ccaccccaac aagctcggga catgaccatc
     3601 ttatgaaaat ttcaagcagt tcaagcactg tggctatccc tggctacacc ttcacaagcc
     3661 cggggatgac caccacccta ccgtcatcgg ttatctcgca aagaactcaa cagacctcca
     3721 gtcagatgcc agccagctct acggcccctt cctcccagga gacagtgcag cccacgagcc
     3781 cggcaacggc attgagaaca cctaccctgc cagagaccat gagctccagc cccacagcag
     3841 catcaactac ccaccgatac cccaaaacac cttctcccac tgtggctcat gagagtaact
     3901 gggcaaagtg tgaggatctt gagacacaga cacagagtga gaagcagctc gtcctgaacc
     3961 tcacaggaaa caccctctgt gcagggggcg cttcggatga gaaattgatc tcactgatat
     4021 gccgagcagt caaagccacc ttcaacccgg cccaagataa gtgcggcata cggctggcat
     4081 ctgttccagg aagtcagacc gtggtcgtca aagaaatcac tattcacact aagctccctg
     4141 ccaaggatgt gtacgagcgg ctgaaggaca aatgggatga actaaaggag gcaggggtca
     4201 gtgacatgaa gctaggggac caggggccac cggaggaggc cgaggaccgc ttcagcatgc
     4261 ccctcatcat caccatcgtc tgcatggcat cattcctgct cctcgtggcg gccctctatg
     4321 gctgctgcca ccagcgcctc tcccagagga aggaccagca gcggctaaca gaggagctgc
     4381 agacagtgga gaatggttac catgacaacc caacactgga agtgatggag acctcttctg
     4441 agatgcagga gaagaaggtg gtcagcctca acggggagct gggggacagc tggatcgtcc
     4501 ctctggacaa cctgaccaag gacgacctgg atgaggagga agacacacac ctctagggat
     4561 ccggaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     4621 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     4681 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     4741 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     4801 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     4861 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     4921 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     4981 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5041 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc
     5101 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacggggacg
     5161 tggttttcct ttgaaaaaca cgatgataag cttgccacaa ccatggccaa gttgaccagt
     5221 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     5281 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     5341 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     5401 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     5461 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     5521 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     5581 gacccggacg ggacgctcct gcgcctgata cagaacgaat tgcttgcagg catctcatga
     5641 gtgtgtcttc ccgttttccg cctgaggtca ctgcgtggat ggagcatcag tggtccaggc
     5701 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
     5761 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     5821 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     5881 agagaagttc agatcaaggt caggaacaga tggaacaggg tcgaccctag agaaccatca
     5941 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     6001 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     6061 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     6121 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     6181 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     6241 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc
     6301 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6361 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6421 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt agatccggct
     6481 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6541 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6601 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6661 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6721 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6781 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttactggct taactatgcg
     6841 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6901 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7981 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8161 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     8221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8461 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     8521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8881 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta
     8941 gcttggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt
     9001 ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     9061 gggtcattag ttcatagccc atatatggag t
//
