LOCUS       Exported                9187 bp ds-DNA     circular SYN 12-MAR-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   David Bryant
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated MMLV gag gene lacking the start codon"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2259..4652
                     /codon_start=1
                     /label=EGFP-hPODXL H484/R486/S488A
                     /translation="MRCALALSALLLLLSTPPLLPSMVSKGEELFTGVVPILVELDGDV
                     NGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQH
                     DFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHK
                     LEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSPSPSPSPSQNATQTTTDSS
                     NKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSG
                     PVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGG
                     KSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLMKISSSSSTV
                     AIPGYTFTSPGMTTTLLETVFHHVSQAGLELLTSGDLPTLASQSAGITASSVISQRTQQ
                     TSSQMPASSTAPSSQETVQPTSPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAH
                     ESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCG
                     IRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAE
                     DRFSMPLIITIVCMASFLLLVAALYGCCAQALAQRKDQQRLTEELQTVENGYHDNPTLE
                     VMETSSEMQEKKVVSLNGELGDSWIVPLDNLTKDDLDEEEDTHL"
     misc_feature    4668..5240
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
     CDS             5299..5673
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5893..6318
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        6584..6913
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6764..6899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(7206..7794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7965..8825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8826..8930)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(9116..9187,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgctgcagga attgatccgc ggccgcaccg gtgccaccat gcgctgcgcg ctggcgctct
     2281 cggcgctgct gctactgttg tcaacgccgc cgctgctgcc gtcgatggtg agcaagggcg
     2341 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2401 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2461 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2521 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2581 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     2641 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2701 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2761 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2821 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2881 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2941 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3001 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtcgccgtcg ccgtcgccgt
     3061 cgccctccca gaatgcaacc cagactacta cggactcatc taacaaaaca gcaccgactc
     3121 cagcatccag tgtcaccatc atggctacag atacagccca gcagagcaca gtccccactt
     3181 ccaaggccaa cgaaatcttg gcctcggtca aggcgaccac ccttggtgta tccagtgact
     3241 caccggggac tacaaccctg gctcagcaag tctcaggccc agtcaacact accgtggcta
     3301 gaggaggcgg ctcaggcaac cctactacca ccatcgagag ccccaagagc acaaaaagtg
     3361 cagacaccac tacagttgca acctccacag ccacagctaa acctaacacc acaagcagcc
     3421 agaatggagc agaagataca acaaactctg gggggaaaag cagccacagt gtgaccacag
     3481 acctcacatc cactaaggca gaacatctga cgacccctca ccctacaagt ccacttagcc
     3541 cccgacaacc cacttcgacg catcctgtgg ccaccccaac aagctcggga catgaccatc
     3601 ttatgaaaat ttcaagcagt tcaagcactg tggctatccc tggctacacc ttcacaagcc
     3661 cggggatgac caccacccta ctagagacag tgtttcacca tgtcagccag gctggtcttg
     3721 aactcctgac ctcgggtgat ctgcccacct tggcctccca aagtgctggg attacagcgt
     3781 catcggttat ctcgcaaaga actcaacaga cctccagtca gatgccagcc agctctacgg
     3841 ccccttcctc ccaggagaca gtgcagccca cgagcccggc aacggcattg agaacaccta
     3901 ccctgccaga gaccatgagc tccagcccca cagcagcatc aactacccac cgatacccca
     3961 aaacaccttc tcccactgtg gctcatgaga gtaactgggc aaagtgtgag gatcttgaga
     4021 cacagacaca gagtgagaag cagctcgtcc tgaacctcac aggaaacacc ctctgtgcag
     4081 ggggcgcttc ggatgagaaa ttgatctcac tgatatgccg agcagtcaaa gccaccttca
     4141 acccggccca agataagtgc ggcatacggc tggcatctgt tccaggaagt cagaccgtgg
     4201 tcgtcaaaga aatcactatt cacactaagc tccctgccaa ggatgtgtac gagcggctga
     4261 aggacaaatg ggatgaacta aaggaggcag gggtcagtga catgaagcta ggggaccagg
     4321 ggccaccgga ggaggccgag gaccgcttca gcatgcccct catcatcacc atcgtctgca
     4381 tggcatcatt cctgctcctc gtggcggccc tctatggctg ctgcgcccag gccctcgccc
     4441 agaggaagga ccagcagcgg ctaacagagg agctgcagac agtggagaat ggttaccatg
     4501 acaacccaac actggaagtg atggagacct cttctgagat gcaggagaag aaggtggtca
     4561 gcctcaacgg ggagctgggg gacagctgga tcgtccctct ggacaacctg accaaggacg
     4621 acctggatga ggaggaagac acacacctct agggatccgg aattccgccc ctctccctcc
     4681 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     4741 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     4801 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     4861 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     4921 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4981 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     5041 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5101 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     5161 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5221 tttgaaaaac acgatgataa gcttgccacg gggacgtggt tttcctttga aaaacacgat
     5281 gataagcttg ccacaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     5341 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     5401 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     5461 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     5521 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     5581 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     5641 tgcgtgcact tcgtggccga ggagcaggac tgacacgacc cggacgggac gctcctgcgc
     5701 ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt tttccgcctg
     5761 aggtcactgc gtggatggag catcagtggt ccaggctcta gttttgactc aacaatatca
     5821 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca
     5881 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     5941 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     6001 aacagatgga acagggtcga ccctagagaa ccatcagatg tttccagggt gccccaagga
     6061 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     6121 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     6181 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     6241 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     6301 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
     6361 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6421 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6481 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     6541 ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg
     6601 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6661 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6721 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6841 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6901 aggcttttgc aaaaagctta ctggcttaac tatgcggcat cagagcagat tgtactgaga
     6961 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7621 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     7681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8101 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8281 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8581 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8941 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     9001 aggcgtatca cgaggccctt tcgtcttcaa gaattagctt ggccattgca tacgttgtat
     9061 ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat
     9121 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     9181 atggagt
//
