LOCUS       Exported               16316 bp ds-DNA     circular SYN 16-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   JZ
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 10, 2023 from SnapGene 5.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(624..1484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1485..1589)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1615..2070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2161..2181
                     /label=PR21-1
     primer_bind     2212..2228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2235..2253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2288..2307
                     /label=BT522
     protein_bind    2304..2373
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    2339..2567
                     /label=AttRB from sequencing
     primer_bind     complement(2394..2414)
                     /label=BT436
     enhancer        2605..2984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2986..3263
                     /label=chicken beta-actin promoter
     intron          3265..4282
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4333..4358
                     /label=oJZ013
     primer_bind     4342..4358
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4372..4405)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     4376..4401
                     /label=oJZ009
     promoter        4435..4931
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4860..4882)
                     /label=oJZ010
     CDS             4945..5748
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     5080..5094
                     /label=Primer 2
     polyA_signal    6079..6213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6332..6466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6585..6719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6718..6740
                     /label=oJZ012
     protein_bind    complement(6748..6781)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     6752..6777
                     /label=oJZ009
     promoter        complement(6875..6893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6904..6920)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            6931..10074
                     /label=AAVR
     primer_bind     6942..6961
                     /label=AU040320-5 MUT
     primer_bind     complement(6963..6978)
                     /label=Primer 1
     primer_bind     complement(6978..7006)
                     /label=oJZ011
     primer_bind     complement(7589..7614)
                     /label=oJZ015
     CDS             10075..10782
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     10075..10093
                     /label=oCD625
     primer_bind     complement(10373..10392)
                     /label=rfp_1r
     CDS             10792..10857
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     regulatory      10873..10882
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             10882..10947
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10954..10974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10999..15099
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             15100..15147
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    15272..15393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    15440..15490
                     /label=attLB from sequencing
     primer_bind     15440..15459
                     /label=BT522
     protein_bind    15456..15525
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(15546..15566)
                     /label=BT436
     promoter        complement(15740..15758)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15779..15795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    15803..15819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(15827..15857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(15830..15851)
                     /label=PR22-1
     protein_bind    15872..15893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(join(16181..16316,1..453))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
       61 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      181 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      301 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     1621 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1681 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1741 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1801 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1861 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1921 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     1981 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2041 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     2101 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     2161 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     2221 cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
     2281 aggtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg
     2341 ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg
     2401 tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg
     2461 ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg
     2521 cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacggt atcgataagc
     2581 ttcccttaat taacctcgag attcgacatt gattattgac tagttattaa tagtaatcaa
     2641 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2701 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2761 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2821 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2881 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2941 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     3001 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     3061 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     3121 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     3181 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     3241 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     3301 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     3361 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     3421 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     3481 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     3541 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     3601 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     3661 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     3721 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     3781 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     3841 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     3901 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     3961 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     4021 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     4081 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     4141 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     4201 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     4261 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     4321 ttttggcaaa gaattggccg ctctagaact agtggatccg gaacccttaa tataacttcg
     4381 tataatgtat gctatacgaa gttattaggt ccctcgacct gcaggaattc taccgggtag
     4441 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctggcacttg
     4501 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta gcgccaaccg
     4561 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     4621 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     4681 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     4741 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     4801 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cgaaggtcct cccgaggccc
     4861 ggcattctcg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     4921 cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg attgcacgca
     4981 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5041 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5101 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5161 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5221 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5281 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5341 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5401 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5461 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc
     5521 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5581 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5641 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5701 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatccgctg
     5761 taagtctgca gaaattgatg atctattaaa caataaagat gtccactaaa atggaagttt
     5821 ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg aatggaagga
     5881 ttggagctac gggggtgggg gtggggtggg attagataaa tgcctgctct ttactgaagg
     5941 ctctttacta ttgctttatg ataatgtttc atagttggat atccttatac cacatttgta
     6001 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     6061 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6181 aaactcatca atgtatctta tcatgtctgg atctgcgact ctgaggatca taatcagcca
     6241 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6301 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6421 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgcg actctgagga
     6481 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6541 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     6601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     6721 ccatcaagct gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     6781 ttagggctag catctgcgac tctgaggatc atatcatgaa tctcgactga attgttcggc
     6841 gcgcctaggc cttgacggcc ttccgccaat tcgccctata gtgagtcgta ttacgtcgcg
     6901 ctcactggcc gtcgttttac aagtttcacc atggagaaga gactgggagt caagccaagt
     6961 cccgcttcct gggttttgcc aggatattgt tggcagacat cagtgaagct gccgagaagc
     7021 ctgtacctgc tttacagttt cttctgcttc agcgttctgt ggttgtcaac agatgctgat
     7081 gagagcagat gccaacaggg gaagacactt tatggagctg gcttgagaac tgagggagaa
     7141 aatcacctcc ggcttcttgc aggaagcctg cctttccacg cctgtcgggc tgcctgctgc
     7201 cgggactctg cctgccacgc tctatggtgg ctggaaggga tgtgctttca ggctgactgc
     7261 agtaagcccc agagctgcca gccttttagg acagactctt ccaattccat gctgatcatt
     7321 tttcaaaaat cccaaactac agatgatttg ggccttctgc ctgaagatga tgaaccacat
     7381 cttctgaggc taggctgggg caggacatcg tggaggaggc agagccttct tggggctccc
     7441 ctcacccttt ctgtaccctc tagtcaccac cagagcttac tcagggatcg gcagaagaga
     7501 gatctcagtg tggtacctac acatggagcg atgcagcatt ctaaagtgaa tcactcggag
     7561 gaagcaggtg ctctgagtcc cacctctgca gaggtccgca aaaccattac agttgccggt
     7621 tccttcacca gtaaccacac tacacagact cctgagtggc ccaagaatgt gtccatccat
     7681 cctgaaccat ccgagcactc cagtcctgta tctggtactc cgcaagtaaa aagcactgag
     7741 cacagtccaa ctgatgcccc tctgccagtg gccccctcct acagctatgc cacccccacg
     7801 ccccaggcct cttctcagag cacctcagca ccacacccag ttgtaaagga gctggtggtg
     7861 tctgctggga agagcgtcca gatcaccctg cctaagaatg aagttcagtt aaatgccttc
     7921 gtccttccag aagcagagcc aggagaaacc tacacctacg actggcagct gatcactcat
     7981 cctacagact acagtggaga ggtggagagg aaacattccc agagcctcca actgtccaag
     8041 ctgactccag gcctgtacga attcaaggtg actgtggatg gccagaatgc ccatggggaa
     8101 ggctacgtga atgtgacagt gaaaccagag ccccgtaaga accggcctcc cgttgctgtg
     8161 gtgtcacctc agttccagga gatctcgctg ccaaccactt ctaccatcat tgatggcagc
     8221 cagagcacgg atgacgataa aattgtccag taccactggg aagagcttaa ggggcccctg
     8281 agagaagaga agatctctga agacacagcc atactaaaac ttagtaagct cgtcccgggg
     8341 aactacacct tcagcttaac tgttgtcgac tctgacgggg ctaccaactc caccactgca
     8401 agcctgactg tgaacaaagc tgtggactac cctcccgtgg ccaatgcagg ccccaaccaa
     8461 gtgatcaccc tgcctcagaa ctccatcacc ctctttggaa accagagcac ggatgaccac
     8521 ggcatcacca gctatgagtg gtcgctcagc ccgagcagca aagggaaggt ggtggagatg
     8581 cagggagtta gaacgccagc cctgcagctg tccgcaatgc aagaaggaga ctatacctac
     8641 cagctcacag tgactgacac cgcaggacaa caggccaccg cccaagtgac tgtgattgtg
     8701 cagcctgaga acaacaagcc tcctcaggca gatgcaggcc cagacaaaga gctgaccctg
     8761 cccgtggaca gcacaaccct ggacggcagc aagagcacag atgaccagag agtcgtctct
     8821 tacctttggg agcagagtcg gggacctgac ggggtgcagc tggagaatgc caacagcagt
     8881 gtcgccactg tgactgggct gcaagtcggg acttatgtat tcaccttgac tgtcaaagat
     8941 gagaggaacc tacagagcca gagctccgtt aatgtcattg tcaaagaaga aataaacaaa
     9001 ccgccagtag ccaagatcgc tgggaacgtg gtggtgacct tgcccacgag cacagctgag
     9061 ctggatggct cgaggtcctc agatgacaag gggatagtca gctacctgtg gactcgagat
     9121 gagacgagcc cagccgcagg ggaggtgctg aatcactctg accaccaccc tgtcctcttc
     9181 ctctccaacc tggtggaggg gacctacacg tttcacctga aagtgacaga tgcaaagggc
     9241 gagagcgaca cagaccggac gacagtggaa gtgaagcctg accccaggaa aagcaaccta
     9301 gtggagatca tcttggatgt gaacgtcagt cagctgactg agaggctgaa ggggatgctc
     9361 atccgccaga ttggggtcct cctgggggtg ctggattccg acatcattgt gcaaaagatt
     9421 cagccgtaca cggagcagag caccaagatg ttgttttttg ttcagaacga ccctccccac
     9481 cagctcttca aaggccatga ggtggcagcc atgctcaaga gcgagctgca gaagcagaag
     9541 gctgacttcc tcatcttcag agccctggaa atcagcacag tcacatgtca gctgaactgt
     9601 tctgaccatg gccactgtga ctcattcacc aagcgctgtg tctgtgaccc gttttggatg
     9661 gagaatttca tcaaggtgca gctgagggat ggagacagca actgtgaatg gagcgtgctc
     9721 tacgtcatca ttgcctcctt tgtcattgtt gttgccttgg ggatcctgtc atggactaca
     9781 atctgctgct gcaagaggca aaaaggaaaa cccaagagga aaagcagata caagatcctg
     9841 gatgccacag atcaggagag cctggagctg aaaccaacct cccgagcagg cagcaaacag
     9901 aaaggcccca cgctgagcag cagcctgatg cattctgaat cggagctgga cagcgacgat
     9961 gccatcttca catggccaga ccgggagaag ggcaaactac tgtatggtca gaatggctct
    10021 gtgccaaacg ggcaaacacc tttgaagtcc aggagcgcac gggaggagat cttgatggtg
    10081 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
    10141 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
    10201 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
    10261 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
    10321 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
    10381 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
    10441 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
    10501 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
    10561 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
    10621 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
    10681 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
    10741 gagggccgcc actccaccgg cggcatggac gagctgtaca agggaagcgg agtgaaacag
    10801 actttgaatt ttgaccttct caagttggcg ggagacgtgg agtccaaccc tggacctccc
    10861 caaacgaccg gtgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat
    10921 attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc
    10981 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct
    11041 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
    11101 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
    11161 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
    11221 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
    11281 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
    11341 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
    11401 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
    11461 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
    11521 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
    11581 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
    11641 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
    11701 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
    11761 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
    11821 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
    11881 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
    11941 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
    12001 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
    12061 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
    12121 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
    12181 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
    12241 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
    12301 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
    12361 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
    12421 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
    12481 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
    12541 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
    12601 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
    12661 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
    12721 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
    12781 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
    12841 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
    12901 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
    12961 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
    13021 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
    13081 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
    13141 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
    13201 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
    13261 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
    13321 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
    13381 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
    13441 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
    13501 tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
    13561 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
    13621 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
    13681 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
    13741 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
    13801 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
    13861 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
    13921 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
    13981 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
    14041 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
    14101 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
    14161 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
    14221 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
    14281 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
    14341 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
    14401 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
    14461 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
    14521 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
    14581 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
    14641 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
    14701 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
    14761 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
    14821 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
    14881 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
    14941 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
    15001 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
    15061 tacgagacac ggatcgacct gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa
    15121 aaggccggcc aggcaaaaaa gaaaaagtaa gaattctaga taactgatca taatcagcca
    15181 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    15241 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    15301 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    15361 ttgtggtttg tccaaactca tcaatgtatc ttatttaaat tgggcgcgcc caactagttc
    15421 tagagcggcc gccactcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag
    15481 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
    15541 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
    15601 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg
    15661 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgagt
    15721 ggagctccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt
    15781 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    15841 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    15901 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    15961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    16021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    16081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    16141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    16201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    16261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgacc
//
