LOCUS       Exported                3077 bp ds-DNA     circular SYN 08-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..140
                     /label=incom term
     misc_feature    141..171
                     /label=H2 homology
     gene            340..1152
                     /label=KanR
     rep_origin      1215..1802
                     /label=ori
                     /note="ori"
     misc_feature    1890..1925
                     /label=H1 homology
     promoter        1926..2354
                     /label=yibDp
     gene            2355..3074
                     /label=roGFP2
ORIGIN
        1 gctcttctaa tacgactcac tatagggccg gcttatcggt cagtttcacc tgatttacgt
       61 aaaaacccgc ttcggcgggt ttttgctttt ggaggggcag aaagatgaat gactgtccac
      121 gacgctatac ccaaaagaaa gacgaattct ctagatatcg ctcaatactg accatttaaa
      181 tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat
      241 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      301 tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa
      361 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      421 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
      481 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      541 gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      601 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
      661 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
      721 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt
      781 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat
      841 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
      901 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
      961 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1021 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1081 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc
     1141 gatgagtttt tctgagggcc caaatgtaat cacctggctc accttcgggt gggcctttct
     1201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc
     1261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1381 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1561 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1621 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1681 ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1801 aagaagatcc tttgattttc taccgaagaa aggcccaccc gtgaaggtga gccagtgagt
     1861 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
     1921 tcgtttgccc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2041 gcctttctgc gtttatacac agctaacacc acgtcgtccc tatctgctgc cctaggtcta
     2101 tgagtggttg ctggataacg tgcgtaattg tgctgatctc ttatatagct gctctcatta
     2161 tctctctacc ctgaagtgac tctctcacct gtaaaaataa tatctcacag gcttaatagt
     2221 ttcttaatac aaagcctgta aaacgtcagg ataacttcta tattcaggga gaccacaacg
     2281 gtttccctct acaaataatt ttgtttaact ttcgtagaag agcacttcac acttctggaa
     2341 aaaggagata taccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2401 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2461 gcgatgccac ctacggcaag ctgaccctga agttcatctc caccaccggc aagctgcccg
     2521 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     2581 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2641 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2701 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2761 acatcctggg gcacaagctg gagtacaact acaactgcca caacgtctat atcatggccg
     2821 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2881 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2941 tgcccgacaa ccactacctg agcacctgct ccgccctgag caaagacccc aacgagaagc
     3001 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3061 agctgtacaa gtaatga
//
