LOCUS       Exported                1705 bp ds-DNA     circular SYN 12-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(181..816)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(984..1008)
                     /label=JNH148
     primer_bind     1009..1026
                     /label=JNH34
     terminator      1025..1052
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1058..1148
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    1163..1696
                     /label=GmR
ORIGIN
        1 gaagtgccag acgatggaag gacgcaatga agctggagga ttggcgtggg aatcgtgctt
       61 ctgtctaagc aagaatgcct agcgtacagg gtgcactttg taacgatttg ggagtccaga
      121 gactcgctgt tttcgaaatt ctcggtcgct acgctccggg cgtgagacta tgcagcgcag
      181 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
      241 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      421 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      481 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      541 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      601 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttggtatctg
      661 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      721 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag
      781 gatctcaaga agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
      841 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
      901 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
      961 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgtgg atccgagtca
     1021 cactggctca ccttcgggtg ggcctttctg cgtttattga tcggcacgta agaggttcca
     1081 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     1141 caggagctgg aaggaagtaa aaatgttgcg tagctctaac gatgtgacgc aacaaggttc
     1201 gcgtccaaag acaaaattgg gaggcagtag catggggatc attcgcactt gtcgcctggg
     1261 gccagaccag gtgaagtcaa tgcgtgcggc tctggactta ttcgggcgcg aatttggaga
     1321 tgtagccact tactcacagc accaaccgga cagtgattac ttggggaatt tacttcgcag
     1381 taaaactttt atcgctttgg ccgctttcga ccaggaggct gtagtaggtg cgttggcagc
     1441 ctatgttctt cctaaattcg agcaaccgcg tagcgaaatt tacatctatg atcttgcagt
     1501 ctccggcgaa catcgccgtc aggggatcgc cacagcttta atcaaccttt tgaagcatga
     1561 ggctaatgca cttggagcgt acgtgattta tgtgcaggct gactacggtg atgatcctgc
     1621 agtcgctctg tacaccaaac tgggtatccg cgaggaggtc atgcactttg atattgaccc
     1681 gtctacggct acctaataga agctt
//
