LOCUS       Exported                6061 bp ds-DNA     circular SYN 11-MAY-2023
DEFINITION  pOTTC1664 was constructed by Laura Owlett (UR) and Chris Richie at 
            GEVVC/NIDA  (CR2028_A).backbone was pOTTC1479 was constructed by 
            Chris Richie at GE&VVC/NIDA (CR1712_C).CR1712 - pAAV SYN1 iRFP-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     promoter        156..640
                     /label=SYN1 promoter
     primer_bind     572..591
                     /label=hSYN1p F417
     CDS             671..2110
                     /label=mGas6(F50-E275 delta)-Myc-DDK
     modified_base   815..820
                     /label=Gas6 deletion breakpoint
     protein_bind    2033..2062
                     /label=Myc epitope [EQKLISEEDL]
     protein_bind    2081..2104
                     /label=FLAG epitope / EK cleavage site [DYKDDDDK]
     misc_RNA        2178..2786
                     /label=WPRE
     primer_bind     complement(2299..2318)
                     /label=WPRE R1
     polyA_signal    2807..3287
                     /label=hGH polyA
     LTR             complement(3324..3464)
                     /label=ITR (AAV)
     rep_origin      3539..3994
                     /label=M13 origin
     rep_origin      3556..3862
                     /label=f1 origin
     promoter        4282..4380
                     /label=Bla promoter (prokaryotic)
     CDS             4381..5238
                     /label=AmpR
     rep_origin      5336..6018
                     /label=ColE1 origin
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgaagttat
      661 cgctagcacc atgccgccac cgcccgggcc cgccgccgcc ctgggcactg cgcttctgct
      721 gctcctgctg gcttccgagt cttctcacac tgtgctgttg cgggcgcgtg aggcggcgca
      781 gtttctgcgg cccaggcagc gccgcgccta ccaagtcgac atcttaccat gtgtgccctt
      841 cagcatggcc aagagcgtga agtccttgta cctgggccgc atgttcagcg ggacccccgt
      901 gattagacta cgcttcaaga ggcttcagcc taccaggctg ctggctgaat ttgacttccg
      961 cacttttgac cctgaaggag tcctcttctt cgctggaggc cgttcagaca gcacctggat
     1021 tgtcctgggc ctaagagctg ggcggcttga gctgcagctt cggtacaatg gcgttgggcg
     1081 catcaccagc agcgggccaa ccatcaacca cggcatgtgg caaactatct ccgtggaaga
     1141 gctggaacgt aaccttgtca tcaaggtcaa caaagatgct gtaatgaaga tcgcggtagc
     1201 tggggagctg tttcagctgg agaggggcct ctatcacctg aatctcaccg tgggcggcat
     1261 tcccttcaag gagagtgagc tcgtccagcc gattaaccct cgcctggatg ggtgcatgag
     1321 gagttggaac tggctgaacg gggaagacag cgccatccag gagacagtca aggcaaacac
     1381 aaaaatgcag tgcttctctg tgacagaaag gggctccttc ttcccgggga atggatttgc
     1441 tacctacagg ctcaactaca cccgaacatc gctggatgtc ggcacggaaa ccacctggga
     1501 agttaaagtt gtggctcgga tccgccctgc cacggacacg ggggtgctgc tggcgctggt
     1561 gggggacgac gatgtcgtcc ccatctctgt ggccctagtc gactaccact ctacaaagaa
     1621 gctcaagaag cagttggtgg tcctggcagt tgaggatgtt gccctggcac tgatggaaat
     1681 caaggtgtgc gacagccagg aacacacggt cactgtctcc ctgcgggagg gtgaggccac
     1741 cctagaagtg gatggcacaa agggccagag tgaagtgagc actgcccagc tgcaggagcg
     1801 actggacaca cttaagacac atctgcaagg ctctgtgcac acctatgttg gaggcctgcc
     1861 agaagtatcg gtgatttctg cacccgtcac tgcgttctac cgcggatgca tgactctgga
     1921 ggtaaacggg aaaatcctgg acctggatac ggcctcgtac aagcacagtg acatcacctc
     1981 ccactcctgc ccgcctgtgg agcatgccac ccccacgcgt acgcggccgc tcgagcagaa
     2041 actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg atgacgacga
     2101 taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatggc gcgccataac
     2161 ttcgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2221 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2281 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2341 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2401 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2461 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2521 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2581 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     2641 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2701 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2761 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     2821 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2881 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2941 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3001 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3061 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     3121 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     3181 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     3241 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     3301 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3361 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3421 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3481 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3721 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3841 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     3901 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3961 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4021 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4081 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4141 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4501 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4561 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4621 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4681 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4741 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4801 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4921 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5101 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5161 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5221 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5521 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6061 t
//
