LOCUS       Exported                6967 bp ds-DNA     circular SYN 20-APR-2022
DEFINITION  pOTTC2185 was constructed by Chris Richie at NIDA GEVVC 
            (CR2998_A).backbone was pOTTC2147 cosntructed by Carly Buker at 
            GEVVC/NIDA (CYB152_C, CR2869).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   NIDAGEVVC - Carly Buker
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     LTR             1..141
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     LTR             1..117
                     /label=scITR (AAV, OTTC trs deletion)
     variation       17..57
                     /label=AAV ITR site of internal deletion (AAAGCCCGGGC) fo
     misc_feature    158..701
                     /locus_tag="EF1"
                     /label=EF1
     primer_bind     complement(158..176)
                     /label=SysBio EF1a R21
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(173..195)
                     /label="CR1539 EFS probe (FAM, Zen)"
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(184..200)
                     /label=EF1a R29
                     /note="primer_bind	steel blue	orange	0	0"
     promoter        188..680
                     /locus_tag="nEF promoter"
                     /label=nEF promoter
                     /note="Abbreviated (shorter) version of human EF1a 
                     promoter"
     primer_bind     329..356
                     /label=EFS F185
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     336..360
                     /label=EFS_F192
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     376..396
                     /label=EFS F232
                     /note="primer_bind	steel blue	orange	0	0"
     LTR             412..680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_recomb     708..741
                     /locus_tag="F3"
                     /label=F3
     misc_feature    742..788
                     /label=direct repeat intra recombination region
     misc_recomb     792..825
                     /locus_tag="F5"
                     /label=F5
     misc_feature    814..833
                     /label=AscI linker
     primer_bind     815..833
                     /label=CR2869_AscI_ex3_rev
     regulatory      834..843
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             840..1256
                     /codon_start=1
                     /label=hChR2(H134R)-NT
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLT"
     primer_bind     complement(930..955)
                     /label=ChR2 R116
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     1205..1225
                     /label=hChR2 F366
                     /note="primer_bind	steel blue	orange	0	0"
     intron          1258..1278
                     /label=INTRSECT splice donor for intron 1
                     /note="previously labeled CE donor (INTRSECT)"
     protein_bind    1279..1312
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     misc_recomb     1279..1312
                     /label=loxN
     primer_bind     1287..1312
                     /label=CR2869 ex2 For
     protein_bind    1363..1396
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     misc_recomb     1363..1396
                     /locus_tag="loxP* (modified loxP to retain TATA box)"
                     /label=loxP* (modified loxP to retain TATA box)
     primer_bind     1370..1416
                     /label=CR2869 ex2 Rev
     intron          1403..1416
                     /label=CE donor (INTRSECT)
     intron          complement(1403..1416)
                     /label=INTRSECT splice donor for intron 2
                     /note="(previously labeled CE donor (INTRSECT)"
     CDS             complement(1417..1476)
                     /codon_start=1
                     /product="mCherry NT for INTRSECT"
                     /label=mCherry (NT)
                     /translation="PPXPPXXXPPXXPPXXPXLP"
     primer_bind     complement(1456..1476)
                     /label=EGFP F1
                     /note="primer_bind	steel blue	orange	0	0"
     regulatory      1473..1482
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(1492..2001)
                     /codon_start=1
                     /label=hCHR2(H134R)-CT
                     /translation="GLSNDYSRRTMGLLVSDIGTIVWGATSAMATGYVKVIFFCLGLCY
                     GANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVSWGMFPILFILGPEGFGVLSVY
                     GSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIRKTTKLNIGGTEIEVETLVED
                     EAEAGAV"
     primer_bind     1789..1808
                     /label=ChR2 R630
                     /note="primer_bind	steel blue	orange	0	0"
     intron          2001..2096
                     /label=INTRSECT splice acceptor for intron 1
     primer_bind     complement(2092..2128)
                     /label=CR2869 ex2 For
     protein_bind    complement(2103..2136)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     misc_recomb     2103..2136
                     /label=loxN
     misc_feature    2140..2186
                     /label=direct repeat intra recombination region
     primer_bind     complement(2186..2213)
                     /label=CR2869 ex2 Rev
     protein_bind    complement(2187..2220)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     misc_recomb     complement(2187..2220)
                     /locus_tag="loxP* (modified loxP to retain TATA box)"
                     /label=loxP* (modified loxP to retain TATA box)
     primer_bind     2210..2246
                     /label=CR2869_SalI_ex3_for
     intron          2227..2294
                     /label=INTRSECT splice acceptor for intron 2
                     /note="previously labeled CE donor/acceptor (INTRSECT)"
     primer_bind     complement(2294..2311)
                     /label=mCherry R77
                     /note="primer_bind	steel blue	orange	0	0"
     CDS             2295..2945
                     /codon_start=1
                     /product="mCherry CT for INTRSECT"
                     /label=mCherry (CT)
                     /translation="PPPPPGGGPPGGGPPPPPGGGGGGGGGGGGGG*XPRPPGGGGGGG
                     PGGPPPPPPGGGGGGGGGGGGPPPGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
                     GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
                     GGGGGGGGGREGGGGGGRGGGGGGGGEPPPPPPPXPGGGGGGEGGGGGGGGGGG"
     primer_bind     complement(2447..2466)
                     /label=mCherry R232
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2514..2533
                     /label=DsRed2 Seq For
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2514..2533
                     /label=mCherry F280
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2514..2533
                     /label=mCherry F280 (1)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2514..2533
                     /label=mCherry F280 (2)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2514..2533
                     /label=mCherry F280 (3)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2774..2793
                     /label=YZ1005 DiomCherry PR
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(2844..2867)
                     /label=YZ1005 DiomCherry Probe
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(2868..2888)
                     /label=YZ1005 DiomCherry PF
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(2922..2962)
                     /label=CR2869_AscI_ex3_rev
     misc_feature    2946..2969
                     /label=XbaI linker
     misc_recomb     complement(2952..2985)
                     /locus_tag="F3(1)"
                     /label=F3
                     /label=F3(1)
     misc_recomb     complement(3036..3069)
                     /locus_tag="F5(1)"
                     /label=F5
                     /label=F5(1)
     misc_RNA        3084..3692
                     /locus_tag="WPRE"
                     /label=WPRE
     misc_feature    3094..3682
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3205..3224)
                     /label=WPRE R1
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(3205..3224)
                     /label=WPRE R1 (1)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(3205..3224)
                     /label=WPRE R1 (2)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     3449..3470
                     /label=WPRE F366
                     /note="primer_bind	steel blue	orange	0	0"
     CDS             complement(3565..3576)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3713..4193
                     /locus_tag="hGH polyA"
                     /label=hGH polyA
     primer_bind     complement(3818..3850)
                     /label=hGHpA R139
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     4090..4109
                     /label=hGHpA F379
                     /note="primer_bind	steel blue	orange	0	0"
     LTR             complement(4230..4370)
                     /locus_tag="ITR (AAV)(1)"
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     repeat_region   4230..4370
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     LTR             4254..4370
                     /label=scITR (AAV, OTTC trs deletion)
     variation       4314..4354
                     /label=AAV ITR site of internal deletion (AAAGCCCGGGC) fo
     rep_origin      4445..4900
                     /locus_tag="M13 origin"
                     /label=M13 origin
     rep_origin      4462..4768
                     /locus_tag="f1 origin"
                     /label=f1 origin
     variation       4493..4522
                     /label=SNP Found to end with T in CYB090_B
     primer_bind     complement(4573..4594)
                     /label=F1 origin seq REV
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     4734..4766
                     /label=f1 origin seq F
                     /note="primer_bind	steel blue	orange	0	0"
     variation       4748..4777
                     /label=SNP Found to end with T in CYB090_B
     variation       4797..4826
                     /label=SNP Found to end with T in CYB090_B
     primer_bind     complement(4917..4936)
                     /locus_tag="pRS-marker"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     promoter        5182..5286
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5188..5286
                     /locus_tag="Bla promoter (prokaryotic)"
                     /label=Bla promoter (prokaryotic)
     CDS             5287..6144
                     /locus_tag="AmpR"
                     /label=AmpR
     primer_bind     complement(5345..5368)
                     /label=pPolyII Left-For
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     5774..5794
                     /label=AmpR qF488
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     5774..5794
                     /label=CR1322 AmpR F488
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     5840..5869
                     /label=CR1322 AmpR probe
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(5875..5894)
                     /label=AmprR qR608
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(5875..5894)
                     /label=CR1322 AmpR R608
                     /note="primer_bind	steel blue	orange	0	0"
     rep_origin      6242..6924
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      6292..6965
                     /label=pUC origin
     rep_origin      6295..6962
                     /label=pUC ori
     rep_origin      6302..6921
                     /label=pBR322 origin
     primer_bind     6435..6461
                     /label=colE1 F194
                     /note="primer_bind	steel blue	orange	0	0"
     variation       6436..6465
                     /label=SNP Found to end with T in CYB090_B
     primer_bind     6440..6461
                     /label=ColE1 qF199
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(6501..6519)
                     /label=ColE1 qR278
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     6601..6625
                     /label=pCR319 REV
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     6843..6862
                     /label=pCR319 att3 REV
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(6849..6873)
                     /label=colE1 R632
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     6890..6910
                     /label=pPolyII Right-Rev
                     /note="primer_bind	steel blue	orange	0	0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 ttcaaatagt ataggaactt cgcagaatgg tagctggatt gtagctgcta ttagcaatat
      781 gaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccgccacca
      841 tggactatgg cggcgctttg tctgccgtcg gacgcgaact tttgttcgtt actaatcctg
      901 tggtggtgaa cgggtccgtc ctggtccctg aggatcaatg ttactgtgcc ggatggattg
      961 aatctcgcgg cacgaacggc gctcagaccg cgtcaaatgt cctgcagtgg cttgcagcag
     1021 gattcagcat tttgctgctg atgttctatg cctaccaaac ctggaaatct acatgcggct
     1081 gggaggagat ctatgtgtgc gccattgaaa tggttaaggt gattctcgag ttcttttttg
     1141 agtttaagaa tccctctatg ctctaccttg ccacaggaca ccgggtgcag tggctgcgct
     1201 atgcagagtg gctgctcact tgtcctgtca tccttatccg cctgagcaac ctcaccggta
     1261 agtgtcgggg ttgctagcat aacttcgtat agtatacctt atacgaagtt attctttgca
     1321 ccattctaaa gaataacagt gataatttct gggttaaggc aaataacttc gtataggata
     1381 ctttatacga agttatggta cccctcctgt actcaccttg aagcgcatga actccttgat
     1441 gatggccatg ttatcctcct cgcccttgct caccatggtg gcggccgctg gcacggctcc
     1501 ggcctcggct tcgtcttcga cgagagtctc gacctcgatc tccgttccgc cgatgttcag
     1561 tttggtggtt ttgcggatat ctccgtgaat caatatgtgc tcgtggatca ggacgcgcag
     1621 gtagtgtccc aacaaccccc aacaattttt actcatcaga tcaataatcg tgtgacctac
     1681 ggtggagcca tagacgctca ggacgccaaa accttcgggc cccaaaatga agagaattgg
     1741 gaacataccc cagctcacga aaaacagcca tgccatgccg gtcacgacct ggcggcaccg
     1801 accctttggc acagtatgat aaccctcgat atatgctttg gcggcgtgaa aaaatgtgtt
     1861 cgcgccatag cacaatccaa gacaaaagaa gatgacttta acatagccgg ttgccatggc
     1921 gctggtagcc ccccacacga tagtcccgat gtctgagaca aggagtccca tggttctcct
     1981 gctgtagtcg ttgctcaggc ctaaacacat gagaaataga gtcataagca catgggtcac
     2041 atacaggaga tatgtatata acattaatac aatttataaa aaaaaggggg ggcacaacta
     2101 gtataacttc gtataaggta tactatacga agttattaag aggtttcata ttgctaatag
     2161 cagctacaat ccagctacca ttctgcataa cttcgtataa agtatcctat acgaagttat
     2221 gtcgactgga gagtggccag cccttctcat gttcagagaa catggttaac tggttaagtc
     2281 atgtcgtccc acaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     2341 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     2401 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     2461 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     2521 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2581 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2641 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     2701 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     2761 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2821 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2881 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2941 agtaatctag agaagttcct atactatttg aagaatagga acttcttgcc ttaacccaga
     3001 aattatcact gttattcttt agaatggtgc aaagagaagt tcctatacct tttgaagaat
     3061 aggaacttcg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     3121 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3181 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3241 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3301 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3361 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3421 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3481 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     3541 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3601 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3661 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
     3721 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     3781 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3841 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3901 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3961 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     4021 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     4081 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     4141 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     4201 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4261 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4321 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4381 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4441 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4501 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4741 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     4801 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4861 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4921 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4981 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5041 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5101 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6961 cacatgt
//
