LOCUS       Exported                6967 bp ds-DNA     circular SYN 25-FEB-2022
DEFINITION  pOTTC2186 was constructed by Chris Richie at NIDA GEVVC 
            (CR2999_C).backbone was pOTTC2147 cosntructed by Carly Buker at 
            GEVVC/NIDA (CYB152_C, CR2869).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   NIDAGEVVC - Carly Buker
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     LTR             1..141
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     LTR             1..117
                     /label=scITR (AAV, OTTC trs deletion)
     variation       17..57
                     /label=AAV ITR site of internal deletion (AAAGCCCGGGC) fo
     misc_feature    158..701
                     /locus_tag="EF1"
                     /label=EF1
     primer_bind     complement(158..176)
                     /label=SysBio EF1a R21
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(173..195)
                     /label="CR1539 EFS probe (FAM, Zen)"
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(184..200)
                     /label=EF1a R29
                     /note="primer_bind	steel blue	orange	0	0"
     promoter        188..680
                     /locus_tag="nEF promoter"
                     /label=nEF promoter
                     /note="Abbreviated (shorter) version of human EF1a 
                     promoter"
     primer_bind     329..356
                     /label=EFS F185
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     336..360
                     /label=EFS_F192
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     376..396
                     /label=EFS F232
                     /note="primer_bind	steel blue	orange	0	0"
     LTR             412..680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_recomb     708..741
                     /locus_tag="F3"
                     /label=F3
     misc_feature    742..788
                     /label=direct repeat intra recombination region
     misc_recomb     792..825
                     /locus_tag="F5"
                     /label=F5
     primer_bind     815..857
                     /label=CR2869_AscI_ex3_rev
     CDS             complement(834..1484)
                     /codon_start=1
                     /product="mCherry CT for INTRSECT"
                     /label=mCherry (CT)
                     /translation="VHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDI
                     LSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFI
                     YKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVK
                     TTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     primer_bind     891..911
                     /label=YZ1005 DiomCherry PF
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     912..935
                     /label=YZ1005 DiomCherry Probe
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(986..1005)
                     /label=YZ1005 DiomCherry PR
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(1246..1265)
                     /label=DsRed2 Seq For
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(1246..1265)
                     /label=mCherry F280
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(1246..1265)
                     /label=mCherry F280 (1)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(1246..1265)
                     /label=mCherry F280 (2)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(1246..1265)
                     /label=mCherry F280 (3)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     1313..1332
                     /label=mCherry R232
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     1468..1485
                     /label=mCherry R77
                     /note="primer_bind	steel blue	orange	0	0"
     intron          1485..1552
                     /label=INTRSECT splice acceptor for intron 2
                     /note="previously labeled CE donor/acceptor (INTRSECT)"
     primer_bind     complement(1533..1569)
                     /label=CR2869_SalI_ex3_for
     protein_bind    1559..1592
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     misc_recomb     1559..1592
                     /locus_tag="loxP* (modified loxP to retain TATA box)"
                     /label=loxP* (modified loxP to retain TATA box)
     primer_bind     1566..1593
                     /label=CR2869 ex2 Rev
     misc_feature    1593..1639
                     /label=direct repeat intra recombination region
     protein_bind    1643..1676
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     misc_recomb     1643..1676
                     /label=loxN
     primer_bind     1650..1696
                     /label=CR2869 ex2 Rev
     misc_feature    1660..1682
                     /label=SpeI linker
     intron          1683..1696
                     /label=CE donor (INTRSECT)
     intron          complement(1683..1696)
                     /label=INTRSECT splice donor for intron 2
                     /note="(previously labeled CE donor (INTRSECT)"
     CDS             complement(1697..1756)
                     /codon_start=1
                     /product="mCherry NT for INTRSECT"
                     /label=mCherry (NT)
                     /translation="MVSKGEEDNMAIIKEFMRFK"
     primer_bind     complement(1736..1756)
                     /label=EGFP F1
                     /note="primer_bind	steel blue	orange	0	0"
     regulatory      1753..1762
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(1772..2281)
                     /codon_start=1
                     /label=hCHR2(H134R)-CT
                     /translation="GLSNDYSRRTMGLLVSDIGTIVWGATSAMATGYVKVIFFCLGLCY
                     GANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVSWGMFPILFILGPEGFGVLSVY
                     GSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIRKTTKLNIGGTEIEVETLVED
                     EAEAGAV"
     primer_bind     2069..2088
                     /label=ChR2 R630
                     /note="primer_bind	steel blue	orange	0	0"
     intron          2281..2376
                     /label=INTRSECT splice acceptor for intron 1
     primer_bind     complement(2377..2409)
                     /label=CR2869 ex2 Rev
     misc_feature    2377..2398
                     /label=KpnI linker
     protein_bind    complement(2383..2416)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     misc_recomb     complement(2383..2416)
                     /locus_tag="loxP* (modified loxP to retain TATA box)"
                     /label=loxP* (modified loxP to retain TATA box)
     protein_bind    complement(2467..2500)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     misc_recomb     2467..2500
                     /label=loxN
     intron          2501..2521
                     /label=INTRSECT splice donor for intron 1
                     /note="previously labeled CE donor (INTRSECT)"
     CDS             complement(2523..2939)
                     /codon_start=1
                     /label=hChR2(H134R)-NT
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLT"
     primer_bind     complement(2554..2574)
                     /label=hChR2 F366
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     2824..2849
                     /label=ChR2 R116
                     /note="primer_bind	steel blue	orange	0	0"
     regulatory      2936..2945
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_recomb     complement(2952..2985)
                     /locus_tag="F3(1)"
                     /label=F3
                     /label=F3(1)
     misc_recomb     complement(3036..3069)
                     /locus_tag="F5(1)"
                     /label=F5
                     /label=F5(1)
     misc_RNA        3084..3692
                     /locus_tag="WPRE"
                     /label=WPRE
     misc_feature    3094..3682
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3205..3224)
                     /label=WPRE R1
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(3205..3224)
                     /label=WPRE R1 (1)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(3205..3224)
                     /label=WPRE R1 (2)
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     3449..3470
                     /label=WPRE F366
                     /note="primer_bind	steel blue	orange	0	0"
     CDS             complement(3565..3576)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3713..4193
                     /locus_tag="hGH polyA"
                     /label=hGH polyA
     primer_bind     complement(3818..3850)
                     /label=hGHpA R139
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     4090..4109
                     /label=hGHpA F379
                     /note="primer_bind	steel blue	orange	0	0"
     LTR             complement(4230..4370)
                     /locus_tag="ITR (AAV)(1)"
                     /label=ITR (AAV)
                     /label=ITR (AAV)(1)
     repeat_region   4230..4370
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     LTR             4254..4370
                     /label=scITR (AAV, OTTC trs deletion)
     variation       4314..4354
                     /label=AAV ITR site of internal deletion (AAAGCCCGGGC) fo
     rep_origin      4445..4900
                     /locus_tag="M13 origin"
                     /label=M13 origin
     rep_origin      4462..4768
                     /locus_tag="f1 origin"
                     /label=f1 origin
     variation       4493..4522
                     /label=SNP Found to end with T in CYB090_B
     primer_bind     complement(4573..4594)
                     /label=F1 origin seq REV
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     4734..4766
                     /label=f1 origin seq F
                     /note="primer_bind	steel blue	orange	0	0"
     variation       4748..4777
                     /label=SNP Found to end with T in CYB090_B
     variation       4797..4826
                     /label=SNP Found to end with T in CYB090_B
     primer_bind     complement(4917..4936)
                     /locus_tag="pRS-marker"
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     promoter        5182..5286
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5188..5286
                     /locus_tag="Bla promoter (prokaryotic)"
                     /label=Bla promoter (prokaryotic)
     CDS             5287..6144
                     /locus_tag="AmpR"
                     /label=AmpR
     primer_bind     complement(5345..5368)
                     /label=pPolyII Left-For
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     5774..5794
                     /label=AmpR qF488
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     5774..5794
                     /label=CR1322 AmpR F488
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     5840..5869
                     /label=CR1322 AmpR probe
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(5875..5894)
                     /label=AmprR qR608
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(5875..5894)
                     /label=CR1322 AmpR R608
                     /note="primer_bind	steel blue	orange	0	0"
     rep_origin      6242..6924
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      6292..6965
                     /label=pUC origin
     rep_origin      6295..6962
                     /label=pUC ori
     rep_origin      6302..6921
                     /label=pBR322 origin
     primer_bind     6435..6461
                     /label=colE1 F194
                     /note="primer_bind	steel blue	orange	0	0"
     variation       6436..6465
                     /label=SNP Found to end with T in CYB090_B
     primer_bind     6440..6461
                     /label=ColE1 qF199
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(6501..6519)
                     /label=ColE1 qR278
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     6601..6625
                     /label=pCR319 REV
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     6843..6862
                     /label=pCR319 att3 REV
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     complement(6849..6873)
                     /label=colE1 R632
                     /note="primer_bind	steel blue	orange	0	0"
     primer_bind     6890..6910
                     /label=pPolyII Right-Rev
                     /note="primer_bind	steel blue	orange	0	0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 ttcaaatagt ataggaactt cgcagaatgg tagctggatt gtagctgcta ttagcaatat
      781 gaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccttacttg
      841 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
      901 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
      961 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
     1021 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
     1081 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
     1141 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
     1201 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
     1261 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
     1321 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
     1381 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
     1441 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcacctgtgg gacgacatga
     1501 cttaaccagt taaccatgtt ctctgaacat gagaagggct ggccactctc cagtcgacat
     1561 aacttcgtat aggatacttt atacgaagtt atgcagaatg gtagctggat tgtagctgct
     1621 attagcaata tgaaacctct taataacttc gtatagtata ccttatacga agttatacta
     1681 gtcctcctgt actcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     1741 cgcccttgct caccatggtg gcggccgctg gcacggctcc ggcctcggct tcgtcttcga
     1801 cgagagtctc gacctcgatc tccgttccgc cgatgttcag tttggtggtt ttgcggatat
     1861 ctccgtgaat caatatgtgc tcgtggatca ggacgcgcag gtagtgtccc aacaaccccc
     1921 aacaattttt actcatcaga tcaataatcg tgtgacctac ggtggagcca tagacgctca
     1981 ggacgccaaa accttcgggc cccaaaatga agagaattgg gaacataccc cagctcacga
     2041 aaaacagcca tgccatgccg gtcacgacct ggcggcaccg accctttggc acagtatgat
     2101 aaccctcgat atatgctttg gcggcgtgaa aaaatgtgtt cgcgccatag cacaatccaa
     2161 gacaaaagaa gatgacttta acatagccgg ttgccatggc gctggtagcc ccccacacga
     2221 tagtcccgat gtctgagaca aggagtccca tggttctcct gctgtagtcg ttgctcaggc
     2281 ctaaacacat gagaaataga gtcataagca catgggtcac atacaggaga tatgtatata
     2341 acattaatac aatttataaa aaaaaggggg ggcacaggta ccataacttc gtataaagta
     2401 tcctatacga agttatttgc cttaacccag aaattatcac tgttattctt tagaatggtg
     2461 caaagaataa cttcgtataa ggtatactat acgaagttat gctagcaacc ccgacactta
     2521 ccggtgaggt tgctcaggcg gataaggatg acaggacaag tgagcagcca ctctgcatag
     2581 cgcagccact gcacccggtg tcctgtggca aggtagagca tagagggatt cttaaactca
     2641 aaaaagaact cgagaatcac cttaaccatt tcaatggcgc acacatagat ctcctcccag
     2701 ccgcatgtag atttccaggt ttggtaggca tagaacatca gcagcaaaat gctgaatcct
     2761 gctgcaagcc actgcaggac atttgacgcg gtctgagcgc cgttcgtgcc gcgagattca
     2821 atccatccgg cacagtaaca ttgatcctca gggaccagga cggacccgtt caccaccaca
     2881 ggattagtaa cgaacaaaag ttcgcgtccg acggcagaca aagcgccgcc atagtccatg
     2941 gtggctctag agaagttcct atactatttg aagaatagga acttcttgcc ttaacccaga
     3001 aattatcact gttattcttt agaatggtgc aaagagaagt tcctatacct tttgaagaat
     3061 aggaacttcg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     3121 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3181 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3241 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3301 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3361 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3421 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3481 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     3541 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3601 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3661 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
     3721 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     3781 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3841 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3901 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3961 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     4021 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     4081 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     4141 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     4201 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4261 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4321 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4381 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4441 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4501 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4741 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     4801 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4861 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4921 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4981 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5041 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5101 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6961 cacatgt
//
